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The phospholipid flippase ALA3 regulates pollen tube growth and guidance in Arabidopsis

Pollen tube guidance regulates the growth direction and ovule targeting of pollen tubes in pistils, which is crucial for the completion of sexual reproduction in flowering plants. The Arabidopsis (Arabidopsis thaliana) pollen-specific receptor kinase (PRK) family members PRK3 and PRK6 are specifical...

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Autores principales: Yang, Yang, Niu, Yue, Chen, Tao, Zhang, Hongkai, Zhang, Jingxia, Qian, Dong, Bi, Mengmeng, Fan, Yuemin, An, Lizhe, Xiang, Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9516151/
https://www.ncbi.nlm.nih.gov/pubmed/35861414
http://dx.doi.org/10.1093/plcell/koac208
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author Yang, Yang
Niu, Yue
Chen, Tao
Zhang, Hongkai
Zhang, Jingxia
Qian, Dong
Bi, Mengmeng
Fan, Yuemin
An, Lizhe
Xiang, Yun
author_facet Yang, Yang
Niu, Yue
Chen, Tao
Zhang, Hongkai
Zhang, Jingxia
Qian, Dong
Bi, Mengmeng
Fan, Yuemin
An, Lizhe
Xiang, Yun
author_sort Yang, Yang
collection PubMed
description Pollen tube guidance regulates the growth direction and ovule targeting of pollen tubes in pistils, which is crucial for the completion of sexual reproduction in flowering plants. The Arabidopsis (Arabidopsis thaliana) pollen-specific receptor kinase (PRK) family members PRK3 and PRK6 are specifically tip-localized and essential for pollen tube growth and guidance. However, the mechanisms controlling the polar localization of PRKs at the pollen tube tip are unclear. The Arabidopsis P4-ATPase ALA3 helps establish the polar localization of apical phosphatidylserine (PS) in pollen tubes. Here, we discovered that loss of ALA3 function caused pollen tube defects in growth and ovule targeting and significantly affected the polar localization pattern of PRK3 and PRK6. Both PRK3 and PRK6 contain two polybasic clusters in the intracellular juxtamembrane domain, and they bound to PS in vitro. PRK3 and PRK6 with polybasic cluster mutations showed reduced or abolished binding to PS and altered polar localization patterns, and they failed to effectively complement the pollen tube-related phenotypes of prk mutants. These results suggest that ALA3 influences the precise localization of PRK3, PRK6, and other PRKs by regulating the distribution of PS, which plays a key role in regulating pollen tube growth and guidance.
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spelling pubmed-95161512022-09-29 The phospholipid flippase ALA3 regulates pollen tube growth and guidance in Arabidopsis Yang, Yang Niu, Yue Chen, Tao Zhang, Hongkai Zhang, Jingxia Qian, Dong Bi, Mengmeng Fan, Yuemin An, Lizhe Xiang, Yun Plant Cell Research Articles Pollen tube guidance regulates the growth direction and ovule targeting of pollen tubes in pistils, which is crucial for the completion of sexual reproduction in flowering plants. The Arabidopsis (Arabidopsis thaliana) pollen-specific receptor kinase (PRK) family members PRK3 and PRK6 are specifically tip-localized and essential for pollen tube growth and guidance. However, the mechanisms controlling the polar localization of PRKs at the pollen tube tip are unclear. The Arabidopsis P4-ATPase ALA3 helps establish the polar localization of apical phosphatidylserine (PS) in pollen tubes. Here, we discovered that loss of ALA3 function caused pollen tube defects in growth and ovule targeting and significantly affected the polar localization pattern of PRK3 and PRK6. Both PRK3 and PRK6 contain two polybasic clusters in the intracellular juxtamembrane domain, and they bound to PS in vitro. PRK3 and PRK6 with polybasic cluster mutations showed reduced or abolished binding to PS and altered polar localization patterns, and they failed to effectively complement the pollen tube-related phenotypes of prk mutants. These results suggest that ALA3 influences the precise localization of PRK3, PRK6, and other PRKs by regulating the distribution of PS, which plays a key role in regulating pollen tube growth and guidance. Oxford University Press 2022-07-21 /pmc/articles/PMC9516151/ /pubmed/35861414 http://dx.doi.org/10.1093/plcell/koac208 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of American Society of Plant Biologists. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Research Articles
Yang, Yang
Niu, Yue
Chen, Tao
Zhang, Hongkai
Zhang, Jingxia
Qian, Dong
Bi, Mengmeng
Fan, Yuemin
An, Lizhe
Xiang, Yun
The phospholipid flippase ALA3 regulates pollen tube growth and guidance in Arabidopsis
title The phospholipid flippase ALA3 regulates pollen tube growth and guidance in Arabidopsis
title_full The phospholipid flippase ALA3 regulates pollen tube growth and guidance in Arabidopsis
title_fullStr The phospholipid flippase ALA3 regulates pollen tube growth and guidance in Arabidopsis
title_full_unstemmed The phospholipid flippase ALA3 regulates pollen tube growth and guidance in Arabidopsis
title_short The phospholipid flippase ALA3 regulates pollen tube growth and guidance in Arabidopsis
title_sort phospholipid flippase ala3 regulates pollen tube growth and guidance in arabidopsis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9516151/
https://www.ncbi.nlm.nih.gov/pubmed/35861414
http://dx.doi.org/10.1093/plcell/koac208
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