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The phospholipid flippase ALA3 regulates pollen tube growth and guidance in Arabidopsis
Pollen tube guidance regulates the growth direction and ovule targeting of pollen tubes in pistils, which is crucial for the completion of sexual reproduction in flowering plants. The Arabidopsis (Arabidopsis thaliana) pollen-specific receptor kinase (PRK) family members PRK3 and PRK6 are specifical...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9516151/ https://www.ncbi.nlm.nih.gov/pubmed/35861414 http://dx.doi.org/10.1093/plcell/koac208 |
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author | Yang, Yang Niu, Yue Chen, Tao Zhang, Hongkai Zhang, Jingxia Qian, Dong Bi, Mengmeng Fan, Yuemin An, Lizhe Xiang, Yun |
author_facet | Yang, Yang Niu, Yue Chen, Tao Zhang, Hongkai Zhang, Jingxia Qian, Dong Bi, Mengmeng Fan, Yuemin An, Lizhe Xiang, Yun |
author_sort | Yang, Yang |
collection | PubMed |
description | Pollen tube guidance regulates the growth direction and ovule targeting of pollen tubes in pistils, which is crucial for the completion of sexual reproduction in flowering plants. The Arabidopsis (Arabidopsis thaliana) pollen-specific receptor kinase (PRK) family members PRK3 and PRK6 are specifically tip-localized and essential for pollen tube growth and guidance. However, the mechanisms controlling the polar localization of PRKs at the pollen tube tip are unclear. The Arabidopsis P4-ATPase ALA3 helps establish the polar localization of apical phosphatidylserine (PS) in pollen tubes. Here, we discovered that loss of ALA3 function caused pollen tube defects in growth and ovule targeting and significantly affected the polar localization pattern of PRK3 and PRK6. Both PRK3 and PRK6 contain two polybasic clusters in the intracellular juxtamembrane domain, and they bound to PS in vitro. PRK3 and PRK6 with polybasic cluster mutations showed reduced or abolished binding to PS and altered polar localization patterns, and they failed to effectively complement the pollen tube-related phenotypes of prk mutants. These results suggest that ALA3 influences the precise localization of PRK3, PRK6, and other PRKs by regulating the distribution of PS, which plays a key role in regulating pollen tube growth and guidance. |
format | Online Article Text |
id | pubmed-9516151 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-95161512022-09-29 The phospholipid flippase ALA3 regulates pollen tube growth and guidance in Arabidopsis Yang, Yang Niu, Yue Chen, Tao Zhang, Hongkai Zhang, Jingxia Qian, Dong Bi, Mengmeng Fan, Yuemin An, Lizhe Xiang, Yun Plant Cell Research Articles Pollen tube guidance regulates the growth direction and ovule targeting of pollen tubes in pistils, which is crucial for the completion of sexual reproduction in flowering plants. The Arabidopsis (Arabidopsis thaliana) pollen-specific receptor kinase (PRK) family members PRK3 and PRK6 are specifically tip-localized and essential for pollen tube growth and guidance. However, the mechanisms controlling the polar localization of PRKs at the pollen tube tip are unclear. The Arabidopsis P4-ATPase ALA3 helps establish the polar localization of apical phosphatidylserine (PS) in pollen tubes. Here, we discovered that loss of ALA3 function caused pollen tube defects in growth and ovule targeting and significantly affected the polar localization pattern of PRK3 and PRK6. Both PRK3 and PRK6 contain two polybasic clusters in the intracellular juxtamembrane domain, and they bound to PS in vitro. PRK3 and PRK6 with polybasic cluster mutations showed reduced or abolished binding to PS and altered polar localization patterns, and they failed to effectively complement the pollen tube-related phenotypes of prk mutants. These results suggest that ALA3 influences the precise localization of PRK3, PRK6, and other PRKs by regulating the distribution of PS, which plays a key role in regulating pollen tube growth and guidance. Oxford University Press 2022-07-21 /pmc/articles/PMC9516151/ /pubmed/35861414 http://dx.doi.org/10.1093/plcell/koac208 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of American Society of Plant Biologists. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Research Articles Yang, Yang Niu, Yue Chen, Tao Zhang, Hongkai Zhang, Jingxia Qian, Dong Bi, Mengmeng Fan, Yuemin An, Lizhe Xiang, Yun The phospholipid flippase ALA3 regulates pollen tube growth and guidance in Arabidopsis |
title | The phospholipid flippase ALA3 regulates pollen tube growth and guidance in Arabidopsis |
title_full | The phospholipid flippase ALA3 regulates pollen tube growth and guidance in Arabidopsis |
title_fullStr | The phospholipid flippase ALA3 regulates pollen tube growth and guidance in Arabidopsis |
title_full_unstemmed | The phospholipid flippase ALA3 regulates pollen tube growth and guidance in Arabidopsis |
title_short | The phospholipid flippase ALA3 regulates pollen tube growth and guidance in Arabidopsis |
title_sort | phospholipid flippase ala3 regulates pollen tube growth and guidance in arabidopsis |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9516151/ https://www.ncbi.nlm.nih.gov/pubmed/35861414 http://dx.doi.org/10.1093/plcell/koac208 |
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