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Mesenchymal Stem Cell: Does it Work in an Experimental Model with Acute Respiratory Distress Syndrome?

We hypothesized that bone marrow-derived mesenchymal stem cells (BM-MSCs) would have a possible role in the treatment of acute respiratory distress syndrome (ARDS). ARDS disease model was developed in Wistar albino male rats by intratracheal instillation of physiological saline solution. Anesthezied...

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Autores principales: Yilmaz, Sema, Inandiklioglu, Nihal, Yildizdas, Dincer, Subasi, Cansu, Acikalin, Arbil, Kuyucu, Yurdun, Bayram, Ibrahim, Topak, Ali, Tanyeli, Atila, Duruksu, Gokhan, Karaoz, Erdal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer New York 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9516527/
https://www.ncbi.nlm.nih.gov/pubmed/22810359
http://dx.doi.org/10.1007/s12015-012-9395-2
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author Yilmaz, Sema
Inandiklioglu, Nihal
Yildizdas, Dincer
Subasi, Cansu
Acikalin, Arbil
Kuyucu, Yurdun
Bayram, Ibrahim
Topak, Ali
Tanyeli, Atila
Duruksu, Gokhan
Karaoz, Erdal
author_facet Yilmaz, Sema
Inandiklioglu, Nihal
Yildizdas, Dincer
Subasi, Cansu
Acikalin, Arbil
Kuyucu, Yurdun
Bayram, Ibrahim
Topak, Ali
Tanyeli, Atila
Duruksu, Gokhan
Karaoz, Erdal
author_sort Yilmaz, Sema
collection PubMed
description We hypothesized that bone marrow-derived mesenchymal stem cells (BM-MSCs) would have a possible role in the treatment of acute respiratory distress syndrome (ARDS). ARDS disease model was developed in Wistar albino male rats by intratracheal instillation of physiological saline solution. Anesthezied and tracheotomized rats (n = 8) with ARDS were pressure-controlled ventilated. Isolated and characterized rat (r-) BM-MSCs were labeled with GFP gene, and introduced in the lungs of the ARDS rat-model. After applying of MSCs, the life span of each rat was recorded. When rats died, their lung tissues were removed for histopathological examination. Also the tissue sections were analyzed for GFP labeled rBM-MSCs and stained for vimentin, CK19, proinflammatory (MPO, IL-1β, IL-6 and MIP-2) and anti-inflammatory [IL-1ra and prostaglandin E2 receptor (EP3)] cytokines. The histopathological signs of rat-model ARDS were similar to the acute phase of ARDS in humans. rBM-MSCs were observed to home in lung paranchyma. Although the infiltration of neutrophils slightly decreased in the interalveolar, peribronchial and perivascular area, a notable improvement was determined in the degree of hemorrhage, edema and hyaline membrane formation in rats treated with rBM-MSCs. Also decreased proinflammatory cytokines levels and increased the intensity of anti-inflammatory cytokines were established. Therefore MSCs could promote alveoar epithelial repair by mediating of cytokines from a proinflammatory to an anti-inflammatory response. As a novel therapeutic approach, mesenchymal stem cell treatment with intratracheal injection could be helpful in the management of critically ill patients with ARDS.
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spelling pubmed-95165272022-09-28 Mesenchymal Stem Cell: Does it Work in an Experimental Model with Acute Respiratory Distress Syndrome? Yilmaz, Sema Inandiklioglu, Nihal Yildizdas, Dincer Subasi, Cansu Acikalin, Arbil Kuyucu, Yurdun Bayram, Ibrahim Topak, Ali Tanyeli, Atila Duruksu, Gokhan Karaoz, Erdal Stem Cell Rev Rep Article We hypothesized that bone marrow-derived mesenchymal stem cells (BM-MSCs) would have a possible role in the treatment of acute respiratory distress syndrome (ARDS). ARDS disease model was developed in Wistar albino male rats by intratracheal instillation of physiological saline solution. Anesthezied and tracheotomized rats (n = 8) with ARDS were pressure-controlled ventilated. Isolated and characterized rat (r-) BM-MSCs were labeled with GFP gene, and introduced in the lungs of the ARDS rat-model. After applying of MSCs, the life span of each rat was recorded. When rats died, their lung tissues were removed for histopathological examination. Also the tissue sections were analyzed for GFP labeled rBM-MSCs and stained for vimentin, CK19, proinflammatory (MPO, IL-1β, IL-6 and MIP-2) and anti-inflammatory [IL-1ra and prostaglandin E2 receptor (EP3)] cytokines. The histopathological signs of rat-model ARDS were similar to the acute phase of ARDS in humans. rBM-MSCs were observed to home in lung paranchyma. Although the infiltration of neutrophils slightly decreased in the interalveolar, peribronchial and perivascular area, a notable improvement was determined in the degree of hemorrhage, edema and hyaline membrane formation in rats treated with rBM-MSCs. Also decreased proinflammatory cytokines levels and increased the intensity of anti-inflammatory cytokines were established. Therefore MSCs could promote alveoar epithelial repair by mediating of cytokines from a proinflammatory to an anti-inflammatory response. As a novel therapeutic approach, mesenchymal stem cell treatment with intratracheal injection could be helpful in the management of critically ill patients with ARDS. Springer New York 2012-07-19 2013 /pmc/articles/PMC9516527/ /pubmed/22810359 http://dx.doi.org/10.1007/s12015-012-9395-2 Text en © Springer Science+Business Media, LLC 2012 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Yilmaz, Sema
Inandiklioglu, Nihal
Yildizdas, Dincer
Subasi, Cansu
Acikalin, Arbil
Kuyucu, Yurdun
Bayram, Ibrahim
Topak, Ali
Tanyeli, Atila
Duruksu, Gokhan
Karaoz, Erdal
Mesenchymal Stem Cell: Does it Work in an Experimental Model with Acute Respiratory Distress Syndrome?
title Mesenchymal Stem Cell: Does it Work in an Experimental Model with Acute Respiratory Distress Syndrome?
title_full Mesenchymal Stem Cell: Does it Work in an Experimental Model with Acute Respiratory Distress Syndrome?
title_fullStr Mesenchymal Stem Cell: Does it Work in an Experimental Model with Acute Respiratory Distress Syndrome?
title_full_unstemmed Mesenchymal Stem Cell: Does it Work in an Experimental Model with Acute Respiratory Distress Syndrome?
title_short Mesenchymal Stem Cell: Does it Work in an Experimental Model with Acute Respiratory Distress Syndrome?
title_sort mesenchymal stem cell: does it work in an experimental model with acute respiratory distress syndrome?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9516527/
https://www.ncbi.nlm.nih.gov/pubmed/22810359
http://dx.doi.org/10.1007/s12015-012-9395-2
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