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Detection of SARS-CoV-2 in Cancellous Bone of Patients with COVID-19 Disease Undergoing Orthopedic Surgery: Laboratory Findings and Clinical Applications

An emerging issue for orthopedic surgeons is how to manage patients with active or previous COVID-19 disease, avoiding any major risks for the surgeons and the O.R. personnel. This monocentric prospective observational study aims to assess the prevalence of SARS-CoV-2 viral RT-PCR RNA in cancellous...

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Autores principales: Guazzoni, Edoardo, Castelli, Alberto, Polizzi, Alberto, Galanzino, Giacomo, Piralla, Antonio, Giardina, Federica, Baldanti, Fausto, Jannelli, Eugenio, Caliogna, Laura, Pasta, Gianluigi, Mosconi, Mario, Grassi, Federico Alberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9517848/
https://www.ncbi.nlm.nih.gov/pubmed/36078337
http://dx.doi.org/10.3390/ijerph191710621
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author Guazzoni, Edoardo
Castelli, Alberto
Polizzi, Alberto
Galanzino, Giacomo
Piralla, Antonio
Giardina, Federica
Baldanti, Fausto
Jannelli, Eugenio
Caliogna, Laura
Pasta, Gianluigi
Mosconi, Mario
Grassi, Federico Alberto
author_facet Guazzoni, Edoardo
Castelli, Alberto
Polizzi, Alberto
Galanzino, Giacomo
Piralla, Antonio
Giardina, Federica
Baldanti, Fausto
Jannelli, Eugenio
Caliogna, Laura
Pasta, Gianluigi
Mosconi, Mario
Grassi, Federico Alberto
author_sort Guazzoni, Edoardo
collection PubMed
description An emerging issue for orthopedic surgeons is how to manage patients with active or previous COVID-19 disease, avoiding any major risks for the surgeons and the O.R. personnel. This monocentric prospective observational study aims to assess the prevalence of SARS-CoV-2 viral RT-PCR RNA in cancellous bone samples in patients with active or previous COVID-19 disease. We collected data about 30 consecutive patients from our institution from January 2021 to March 2021 with active or previous COVID-19 disease. The presence of SARS-CoV-2 in the samples was determined using two different PCR-based assays. Eighteen of the thirty patients included in the study had a positive nasopharyngeal swab at the time of surgery. Twelve patients had a negative nasopharyngeal swab with a mean days since negativization of 138 ± 104 days, ranging from 23 to 331 days. Mean days of positivity to the nasal swab were 17 ± 17. Twenty-nine out of thirty (96.7%) samples were negative for the presence of SARS-CoV-2 RNA. In one sample, low SARS-CoV-2 load (Cycle threshold (Ct) 36.6.) was detected but not confirmed using an additional confirmatory assay. The conducted study demonstrates the absence of the viral genome within the analyzed cancellous bone. We think that the use of personal protection equipment (PPE) to only protect from aerosol produced during surgery, both in active and recovered patients, is not strictly necessary. We think that the use of PPE should not be employed by surgeons and the O.R. personnel to protect themselves from aerosols produced from the respiratory tract. Moreover, we think that our results could represent a valid basis for further studies related to the possibility of bone donation in patients that suffered and recovered from COVID-19.
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spelling pubmed-95178482022-09-29 Detection of SARS-CoV-2 in Cancellous Bone of Patients with COVID-19 Disease Undergoing Orthopedic Surgery: Laboratory Findings and Clinical Applications Guazzoni, Edoardo Castelli, Alberto Polizzi, Alberto Galanzino, Giacomo Piralla, Antonio Giardina, Federica Baldanti, Fausto Jannelli, Eugenio Caliogna, Laura Pasta, Gianluigi Mosconi, Mario Grassi, Federico Alberto Int J Environ Res Public Health Article An emerging issue for orthopedic surgeons is how to manage patients with active or previous COVID-19 disease, avoiding any major risks for the surgeons and the O.R. personnel. This monocentric prospective observational study aims to assess the prevalence of SARS-CoV-2 viral RT-PCR RNA in cancellous bone samples in patients with active or previous COVID-19 disease. We collected data about 30 consecutive patients from our institution from January 2021 to March 2021 with active or previous COVID-19 disease. The presence of SARS-CoV-2 in the samples was determined using two different PCR-based assays. Eighteen of the thirty patients included in the study had a positive nasopharyngeal swab at the time of surgery. Twelve patients had a negative nasopharyngeal swab with a mean days since negativization of 138 ± 104 days, ranging from 23 to 331 days. Mean days of positivity to the nasal swab were 17 ± 17. Twenty-nine out of thirty (96.7%) samples were negative for the presence of SARS-CoV-2 RNA. In one sample, low SARS-CoV-2 load (Cycle threshold (Ct) 36.6.) was detected but not confirmed using an additional confirmatory assay. The conducted study demonstrates the absence of the viral genome within the analyzed cancellous bone. We think that the use of personal protection equipment (PPE) to only protect from aerosol produced during surgery, both in active and recovered patients, is not strictly necessary. We think that the use of PPE should not be employed by surgeons and the O.R. personnel to protect themselves from aerosols produced from the respiratory tract. Moreover, we think that our results could represent a valid basis for further studies related to the possibility of bone donation in patients that suffered and recovered from COVID-19. MDPI 2022-08-25 /pmc/articles/PMC9517848/ /pubmed/36078337 http://dx.doi.org/10.3390/ijerph191710621 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guazzoni, Edoardo
Castelli, Alberto
Polizzi, Alberto
Galanzino, Giacomo
Piralla, Antonio
Giardina, Federica
Baldanti, Fausto
Jannelli, Eugenio
Caliogna, Laura
Pasta, Gianluigi
Mosconi, Mario
Grassi, Federico Alberto
Detection of SARS-CoV-2 in Cancellous Bone of Patients with COVID-19 Disease Undergoing Orthopedic Surgery: Laboratory Findings and Clinical Applications
title Detection of SARS-CoV-2 in Cancellous Bone of Patients with COVID-19 Disease Undergoing Orthopedic Surgery: Laboratory Findings and Clinical Applications
title_full Detection of SARS-CoV-2 in Cancellous Bone of Patients with COVID-19 Disease Undergoing Orthopedic Surgery: Laboratory Findings and Clinical Applications
title_fullStr Detection of SARS-CoV-2 in Cancellous Bone of Patients with COVID-19 Disease Undergoing Orthopedic Surgery: Laboratory Findings and Clinical Applications
title_full_unstemmed Detection of SARS-CoV-2 in Cancellous Bone of Patients with COVID-19 Disease Undergoing Orthopedic Surgery: Laboratory Findings and Clinical Applications
title_short Detection of SARS-CoV-2 in Cancellous Bone of Patients with COVID-19 Disease Undergoing Orthopedic Surgery: Laboratory Findings and Clinical Applications
title_sort detection of sars-cov-2 in cancellous bone of patients with covid-19 disease undergoing orthopedic surgery: laboratory findings and clinical applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9517848/
https://www.ncbi.nlm.nih.gov/pubmed/36078337
http://dx.doi.org/10.3390/ijerph191710621
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