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The diagnostic value of next-generation sequencing technology in sepsis

OBJECTIVE: This study aims to assess the clinical utility of next-generation sequencing (NGS) in sepsis diagnosis. METHODS: A prospective study was conducted on patients with a high suspicion of sepsis by unknown pathogens from January 2017 to December 2021. Blood samples were taken from patients to...

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Autores principales: Cao, Xiao-guang, Zhou, Shu-sheng, Wang, Chun-yan, Jin, Kui, Meng, Hua-dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9518011/
https://www.ncbi.nlm.nih.gov/pubmed/36189371
http://dx.doi.org/10.3389/fcimb.2022.899508
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author Cao, Xiao-guang
Zhou, Shu-sheng
Wang, Chun-yan
Jin, Kui
Meng, Hua-dong
author_facet Cao, Xiao-guang
Zhou, Shu-sheng
Wang, Chun-yan
Jin, Kui
Meng, Hua-dong
author_sort Cao, Xiao-guang
collection PubMed
description OBJECTIVE: This study aims to assess the clinical utility of next-generation sequencing (NGS) in sepsis diagnosis. METHODS: A prospective study was conducted on patients with a high suspicion of sepsis by unknown pathogens from January 2017 to December 2021. Blood samples were taken from patients to perform NGS, blood culture (BC), leucocyte (WBC), procalcitonin (PCT), creatinine (CREA), Albumin (ALB) and C-reactive protein (CRP) tests. RESULTS: The feedback time for BC was 3~5 days for bacteria and 5~7 days for fungi, while the turnover time for NGS was only 24 h. The clinical diagnosis was considered the “gold standard”. 83 patients passed our inclusion criteria and were separated into two groups by clinical diagnosis. 62 met the clinical diagnosis criteria for sepsis and 21 were non-sepsis. The data from the two groups were retrospectively compared and analyzed. Of 62 sepsis in 83 patients, 8(9.64%) were diagnosed by both BC and NGS, 51 (61.45%) by NGS only, 1(1.20%) by BC and 2 (2.41%) by conventional testing only; PCT, CREA, CRP levels and the detection rate of NGS and BC were higher in the sepsis group than in the non-sepsis group, while ALB levels were lower (p<0.05). The logistic regression results in our study revealed that NGS and ALB were independent prediction factors for sepsis (p<0.05), the area under the receiver operating characteristic curve (AUC), sensitivity and specificity of NGS for diagnosing sepsis was 0.857, 95.16% and 76.19%, while ALB was 0.728, 58.06%, 80.95%, respectively. The combination’s sensitivity, specificity and AUC of NGS and ALB were 93.55%, 85.71% and 0.935, greater than that of Albumin or NGS only (both p<0.05). CONCLUSION: NGS can effectively and quickly identify pathogens, thereby emerges as a promising technology for sepsis diagnosis. Combination of NGS and ALB can be used for early screening and is more powerful than NGS or ALB only.
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spelling pubmed-95180112022-09-29 The diagnostic value of next-generation sequencing technology in sepsis Cao, Xiao-guang Zhou, Shu-sheng Wang, Chun-yan Jin, Kui Meng, Hua-dong Front Cell Infect Microbiol Cellular and Infection Microbiology OBJECTIVE: This study aims to assess the clinical utility of next-generation sequencing (NGS) in sepsis diagnosis. METHODS: A prospective study was conducted on patients with a high suspicion of sepsis by unknown pathogens from January 2017 to December 2021. Blood samples were taken from patients to perform NGS, blood culture (BC), leucocyte (WBC), procalcitonin (PCT), creatinine (CREA), Albumin (ALB) and C-reactive protein (CRP) tests. RESULTS: The feedback time for BC was 3~5 days for bacteria and 5~7 days for fungi, while the turnover time for NGS was only 24 h. The clinical diagnosis was considered the “gold standard”. 83 patients passed our inclusion criteria and were separated into two groups by clinical diagnosis. 62 met the clinical diagnosis criteria for sepsis and 21 were non-sepsis. The data from the two groups were retrospectively compared and analyzed. Of 62 sepsis in 83 patients, 8(9.64%) were diagnosed by both BC and NGS, 51 (61.45%) by NGS only, 1(1.20%) by BC and 2 (2.41%) by conventional testing only; PCT, CREA, CRP levels and the detection rate of NGS and BC were higher in the sepsis group than in the non-sepsis group, while ALB levels were lower (p<0.05). The logistic regression results in our study revealed that NGS and ALB were independent prediction factors for sepsis (p<0.05), the area under the receiver operating characteristic curve (AUC), sensitivity and specificity of NGS for diagnosing sepsis was 0.857, 95.16% and 76.19%, while ALB was 0.728, 58.06%, 80.95%, respectively. The combination’s sensitivity, specificity and AUC of NGS and ALB were 93.55%, 85.71% and 0.935, greater than that of Albumin or NGS only (both p<0.05). CONCLUSION: NGS can effectively and quickly identify pathogens, thereby emerges as a promising technology for sepsis diagnosis. Combination of NGS and ALB can be used for early screening and is more powerful than NGS or ALB only. Frontiers Media S.A. 2022-09-14 /pmc/articles/PMC9518011/ /pubmed/36189371 http://dx.doi.org/10.3389/fcimb.2022.899508 Text en Copyright © 2022 Cao, Zhou, Wang, Jin and Meng https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Cao, Xiao-guang
Zhou, Shu-sheng
Wang, Chun-yan
Jin, Kui
Meng, Hua-dong
The diagnostic value of next-generation sequencing technology in sepsis
title The diagnostic value of next-generation sequencing technology in sepsis
title_full The diagnostic value of next-generation sequencing technology in sepsis
title_fullStr The diagnostic value of next-generation sequencing technology in sepsis
title_full_unstemmed The diagnostic value of next-generation sequencing technology in sepsis
title_short The diagnostic value of next-generation sequencing technology in sepsis
title_sort diagnostic value of next-generation sequencing technology in sepsis
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9518011/
https://www.ncbi.nlm.nih.gov/pubmed/36189371
http://dx.doi.org/10.3389/fcimb.2022.899508
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