TiO(2) nanotube immobilised 5-lipoxygenase-mediated screening and isolation of anti-inflammatory active compounds from the leaves of lonicera japonica thunb

In this work, a highly effective separation approach mediated by 5-Lipoxygenase (5-LOX) was established for screening and isolation of anti-inflammatory ingredients from leaves of Lonicera japonica Thunb. (LLJT). Using 5-LOX immobilised on TiO(2) HIGHLIGHTS: A 5-LOX mediated separation method was established for isolation of anti-inflammatory compounds. An anti-inflammatory ingredient was separated by MCI GEL CHP20P column chromatography. One new compound was first isolated from leaves of Lonicera japonica Thunb. 5-LOX was immobilised on TiO(2) nanotubes and exploited by combining with HPLC-MS system. The anti-inflammatory activity of screened components was evaluated. . [Figure: see text]