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The Effect of UV Treatment on Surface Contact Angle, Fibroblast Cytotoxicity, and Proliferation with Two Types of Zirconia-Based Ceramics
UV photofunctionalization of Zirconia-based materials for abutment fabrication is a promising approach that might influence the formation of a sound peri-implant seal, thus promoting long-term soft and hard tissue implant integration. This study aimed to evaluate the effect of UV treatment of test s...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9518570/ https://www.ncbi.nlm.nih.gov/pubmed/36078826 http://dx.doi.org/10.3390/ijerph191711113 |
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author | Rutkunas, Vygandas Borusevicius, Rokas Balciunas, Evaldas Jasinskyte, Urte Alksne, Milda Simoliunas, Egidijus Zlatev, Stefan Ivanova, Vasilena Bukelskiene, Virginija Mijiritsky, Eitan |
author_facet | Rutkunas, Vygandas Borusevicius, Rokas Balciunas, Evaldas Jasinskyte, Urte Alksne, Milda Simoliunas, Egidijus Zlatev, Stefan Ivanova, Vasilena Bukelskiene, Virginija Mijiritsky, Eitan |
author_sort | Rutkunas, Vygandas |
collection | PubMed |
description | UV photofunctionalization of Zirconia-based materials for abutment fabrication is a promising approach that might influence the formation of a sound peri-implant seal, thus promoting long-term soft and hard tissue implant integration. This study aimed to evaluate the effect of UV treatment of test specimens made by two different ZnO(2)-based ceramic materials on the hydrophilicity, cell cytotoxicity, and proliferation of human gingival fibroblasts (HGFs). Two Zirconia-based materials, high-translucent and ultra-translucent multi-layered Zirconia (Katana, Kuraray Noritake, Japan), were used to prepare a total of 40 specimens distributed in two equally sized groups based on the material (n = 20). The same surface finishing protocol was applied for all specimens, as suggested by the manufacturer. Half the specimens from each group were treated with UV-C light for 48 h. Water contact angle (WCA), fibroblast cytotoxicity, and proliferation were investigated. The WCA values for the high-translucent Zirconia ranged from 69.9° ± 6.4° to 73.7° ± 13.9° for the treated/non-treated specimens and from 79.5° ± 12.8° to 83.4° ± 11.4° for the ultra-translucent multi-layered Zirconia, respectively. However, the difference was insignificant (F(16) = 3.50, p = 0.292). No significant difference was observed for the fibroblast cytotoxicity test. The results for proliferation revealed a significant difference, which was material-dependent (F(8) = 9.58, p = 0.005). We found that UV surface photofunctionalization of ZrO(2)-based materials alters the human gingival fibroblast cell viability, which might produce favourable results for cell proliferation. |
format | Online Article Text |
id | pubmed-9518570 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95185702022-09-29 The Effect of UV Treatment on Surface Contact Angle, Fibroblast Cytotoxicity, and Proliferation with Two Types of Zirconia-Based Ceramics Rutkunas, Vygandas Borusevicius, Rokas Balciunas, Evaldas Jasinskyte, Urte Alksne, Milda Simoliunas, Egidijus Zlatev, Stefan Ivanova, Vasilena Bukelskiene, Virginija Mijiritsky, Eitan Int J Environ Res Public Health Article UV photofunctionalization of Zirconia-based materials for abutment fabrication is a promising approach that might influence the formation of a sound peri-implant seal, thus promoting long-term soft and hard tissue implant integration. This study aimed to evaluate the effect of UV treatment of test specimens made by two different ZnO(2)-based ceramic materials on the hydrophilicity, cell cytotoxicity, and proliferation of human gingival fibroblasts (HGFs). Two Zirconia-based materials, high-translucent and ultra-translucent multi-layered Zirconia (Katana, Kuraray Noritake, Japan), were used to prepare a total of 40 specimens distributed in two equally sized groups based on the material (n = 20). The same surface finishing protocol was applied for all specimens, as suggested by the manufacturer. Half the specimens from each group were treated with UV-C light for 48 h. Water contact angle (WCA), fibroblast cytotoxicity, and proliferation were investigated. The WCA values for the high-translucent Zirconia ranged from 69.9° ± 6.4° to 73.7° ± 13.9° for the treated/non-treated specimens and from 79.5° ± 12.8° to 83.4° ± 11.4° for the ultra-translucent multi-layered Zirconia, respectively. However, the difference was insignificant (F(16) = 3.50, p = 0.292). No significant difference was observed for the fibroblast cytotoxicity test. The results for proliferation revealed a significant difference, which was material-dependent (F(8) = 9.58, p = 0.005). We found that UV surface photofunctionalization of ZrO(2)-based materials alters the human gingival fibroblast cell viability, which might produce favourable results for cell proliferation. MDPI 2022-09-05 /pmc/articles/PMC9518570/ /pubmed/36078826 http://dx.doi.org/10.3390/ijerph191711113 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rutkunas, Vygandas Borusevicius, Rokas Balciunas, Evaldas Jasinskyte, Urte Alksne, Milda Simoliunas, Egidijus Zlatev, Stefan Ivanova, Vasilena Bukelskiene, Virginija Mijiritsky, Eitan The Effect of UV Treatment on Surface Contact Angle, Fibroblast Cytotoxicity, and Proliferation with Two Types of Zirconia-Based Ceramics |
title | The Effect of UV Treatment on Surface Contact Angle, Fibroblast Cytotoxicity, and Proliferation with Two Types of Zirconia-Based Ceramics |
title_full | The Effect of UV Treatment on Surface Contact Angle, Fibroblast Cytotoxicity, and Proliferation with Two Types of Zirconia-Based Ceramics |
title_fullStr | The Effect of UV Treatment on Surface Contact Angle, Fibroblast Cytotoxicity, and Proliferation with Two Types of Zirconia-Based Ceramics |
title_full_unstemmed | The Effect of UV Treatment on Surface Contact Angle, Fibroblast Cytotoxicity, and Proliferation with Two Types of Zirconia-Based Ceramics |
title_short | The Effect of UV Treatment on Surface Contact Angle, Fibroblast Cytotoxicity, and Proliferation with Two Types of Zirconia-Based Ceramics |
title_sort | effect of uv treatment on surface contact angle, fibroblast cytotoxicity, and proliferation with two types of zirconia-based ceramics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9518570/ https://www.ncbi.nlm.nih.gov/pubmed/36078826 http://dx.doi.org/10.3390/ijerph191711113 |
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