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The Effect of UV Treatment on Surface Contact Angle, Fibroblast Cytotoxicity, and Proliferation with Two Types of Zirconia-Based Ceramics

UV photofunctionalization of Zirconia-based materials for abutment fabrication is a promising approach that might influence the formation of a sound peri-implant seal, thus promoting long-term soft and hard tissue implant integration. This study aimed to evaluate the effect of UV treatment of test s...

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Autores principales: Rutkunas, Vygandas, Borusevicius, Rokas, Balciunas, Evaldas, Jasinskyte, Urte, Alksne, Milda, Simoliunas, Egidijus, Zlatev, Stefan, Ivanova, Vasilena, Bukelskiene, Virginija, Mijiritsky, Eitan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9518570/
https://www.ncbi.nlm.nih.gov/pubmed/36078826
http://dx.doi.org/10.3390/ijerph191711113
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author Rutkunas, Vygandas
Borusevicius, Rokas
Balciunas, Evaldas
Jasinskyte, Urte
Alksne, Milda
Simoliunas, Egidijus
Zlatev, Stefan
Ivanova, Vasilena
Bukelskiene, Virginija
Mijiritsky, Eitan
author_facet Rutkunas, Vygandas
Borusevicius, Rokas
Balciunas, Evaldas
Jasinskyte, Urte
Alksne, Milda
Simoliunas, Egidijus
Zlatev, Stefan
Ivanova, Vasilena
Bukelskiene, Virginija
Mijiritsky, Eitan
author_sort Rutkunas, Vygandas
collection PubMed
description UV photofunctionalization of Zirconia-based materials for abutment fabrication is a promising approach that might influence the formation of a sound peri-implant seal, thus promoting long-term soft and hard tissue implant integration. This study aimed to evaluate the effect of UV treatment of test specimens made by two different ZnO(2)-based ceramic materials on the hydrophilicity, cell cytotoxicity, and proliferation of human gingival fibroblasts (HGFs). Two Zirconia-based materials, high-translucent and ultra-translucent multi-layered Zirconia (Katana, Kuraray Noritake, Japan), were used to prepare a total of 40 specimens distributed in two equally sized groups based on the material (n = 20). The same surface finishing protocol was applied for all specimens, as suggested by the manufacturer. Half the specimens from each group were treated with UV-C light for 48 h. Water contact angle (WCA), fibroblast cytotoxicity, and proliferation were investigated. The WCA values for the high-translucent Zirconia ranged from 69.9° ± 6.4° to 73.7° ± 13.9° for the treated/non-treated specimens and from 79.5° ± 12.8° to 83.4° ± 11.4° for the ultra-translucent multi-layered Zirconia, respectively. However, the difference was insignificant (F(16) = 3.50, p = 0.292). No significant difference was observed for the fibroblast cytotoxicity test. The results for proliferation revealed a significant difference, which was material-dependent (F(8) = 9.58, p = 0.005). We found that UV surface photofunctionalization of ZrO(2)-based materials alters the human gingival fibroblast cell viability, which might produce favourable results for cell proliferation.
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spelling pubmed-95185702022-09-29 The Effect of UV Treatment on Surface Contact Angle, Fibroblast Cytotoxicity, and Proliferation with Two Types of Zirconia-Based Ceramics Rutkunas, Vygandas Borusevicius, Rokas Balciunas, Evaldas Jasinskyte, Urte Alksne, Milda Simoliunas, Egidijus Zlatev, Stefan Ivanova, Vasilena Bukelskiene, Virginija Mijiritsky, Eitan Int J Environ Res Public Health Article UV photofunctionalization of Zirconia-based materials for abutment fabrication is a promising approach that might influence the formation of a sound peri-implant seal, thus promoting long-term soft and hard tissue implant integration. This study aimed to evaluate the effect of UV treatment of test specimens made by two different ZnO(2)-based ceramic materials on the hydrophilicity, cell cytotoxicity, and proliferation of human gingival fibroblasts (HGFs). Two Zirconia-based materials, high-translucent and ultra-translucent multi-layered Zirconia (Katana, Kuraray Noritake, Japan), were used to prepare a total of 40 specimens distributed in two equally sized groups based on the material (n = 20). The same surface finishing protocol was applied for all specimens, as suggested by the manufacturer. Half the specimens from each group were treated with UV-C light for 48 h. Water contact angle (WCA), fibroblast cytotoxicity, and proliferation were investigated. The WCA values for the high-translucent Zirconia ranged from 69.9° ± 6.4° to 73.7° ± 13.9° for the treated/non-treated specimens and from 79.5° ± 12.8° to 83.4° ± 11.4° for the ultra-translucent multi-layered Zirconia, respectively. However, the difference was insignificant (F(16) = 3.50, p = 0.292). No significant difference was observed for the fibroblast cytotoxicity test. The results for proliferation revealed a significant difference, which was material-dependent (F(8) = 9.58, p = 0.005). We found that UV surface photofunctionalization of ZrO(2)-based materials alters the human gingival fibroblast cell viability, which might produce favourable results for cell proliferation. MDPI 2022-09-05 /pmc/articles/PMC9518570/ /pubmed/36078826 http://dx.doi.org/10.3390/ijerph191711113 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rutkunas, Vygandas
Borusevicius, Rokas
Balciunas, Evaldas
Jasinskyte, Urte
Alksne, Milda
Simoliunas, Egidijus
Zlatev, Stefan
Ivanova, Vasilena
Bukelskiene, Virginija
Mijiritsky, Eitan
The Effect of UV Treatment on Surface Contact Angle, Fibroblast Cytotoxicity, and Proliferation with Two Types of Zirconia-Based Ceramics
title The Effect of UV Treatment on Surface Contact Angle, Fibroblast Cytotoxicity, and Proliferation with Two Types of Zirconia-Based Ceramics
title_full The Effect of UV Treatment on Surface Contact Angle, Fibroblast Cytotoxicity, and Proliferation with Two Types of Zirconia-Based Ceramics
title_fullStr The Effect of UV Treatment on Surface Contact Angle, Fibroblast Cytotoxicity, and Proliferation with Two Types of Zirconia-Based Ceramics
title_full_unstemmed The Effect of UV Treatment on Surface Contact Angle, Fibroblast Cytotoxicity, and Proliferation with Two Types of Zirconia-Based Ceramics
title_short The Effect of UV Treatment on Surface Contact Angle, Fibroblast Cytotoxicity, and Proliferation with Two Types of Zirconia-Based Ceramics
title_sort effect of uv treatment on surface contact angle, fibroblast cytotoxicity, and proliferation with two types of zirconia-based ceramics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9518570/
https://www.ncbi.nlm.nih.gov/pubmed/36078826
http://dx.doi.org/10.3390/ijerph191711113
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