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Genome mining to unravel potential metabolic pathways linked to gallium bioleaching ability of bacterial mine isolates

Gallium (Ga) is considered a high-tech Critical Metal, used in the manufacture of several microelectronic components containing either gallium arsenide (GaAs) or gallium nitride (GaN). The current high demand for this critical metal urges the development of effective recovery processes from secondar...

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Autores principales: Chung, Ana Paula, Francisco, Romeu, Morais, Paula V., Branco, Rita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9518604/
https://www.ncbi.nlm.nih.gov/pubmed/36188007
http://dx.doi.org/10.3389/fmicb.2022.970147
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author Chung, Ana Paula
Francisco, Romeu
Morais, Paula V.
Branco, Rita
author_facet Chung, Ana Paula
Francisco, Romeu
Morais, Paula V.
Branco, Rita
author_sort Chung, Ana Paula
collection PubMed
description Gallium (Ga) is considered a high-tech Critical Metal, used in the manufacture of several microelectronic components containing either gallium arsenide (GaAs) or gallium nitride (GaN). The current high demand for this critical metal urges the development of effective recovery processes from secondary resources such as mine tailings or electronic recycling material. The importance of bioleaching as a biotechnological process to recover metals prompted this study, where an integrative approach combining experimental and genomic analysis was undertaken to identify potential mechanisms involved in bioleaching ability and strategies to cope with high metal(loid)s concentrations in five mine isolates. The Clusters of Orthologous Group (COG) annotation showed that the “amino acid transport and metabolism” [E] was the most predominant functional category in all genomes. In addition, the KEEG pathways analysis also showed predicted genes for the biosynthetic pathways of most amino acids, indicating that amino acids could have an important role in the Ga leaching mechanism. The presence of effective resistance mechanisms to Ga and arsenic (As) was particularly important in GaAs bioleaching batch assays, and might explain the divergence in bioleaching efficiency among the bacterial strains. Rhodanobacter sp. B2A1Ga4 and Sphingomonas sp. A2-49 with higher resistance, mainly to As, were the most efficient bioleaching strains under these conditions. In bioleaching assays using cell-free spent medium Arthrobacter silviterrae A2-55 with lower As resistance outperformed all the other stains. Overall, higher efficiency in Ga leaching was obtained in bioleaching assays using GaAs when compared to GaN.
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spelling pubmed-95186042022-09-29 Genome mining to unravel potential metabolic pathways linked to gallium bioleaching ability of bacterial mine isolates Chung, Ana Paula Francisco, Romeu Morais, Paula V. Branco, Rita Front Microbiol Microbiology Gallium (Ga) is considered a high-tech Critical Metal, used in the manufacture of several microelectronic components containing either gallium arsenide (GaAs) or gallium nitride (GaN). The current high demand for this critical metal urges the development of effective recovery processes from secondary resources such as mine tailings or electronic recycling material. The importance of bioleaching as a biotechnological process to recover metals prompted this study, where an integrative approach combining experimental and genomic analysis was undertaken to identify potential mechanisms involved in bioleaching ability and strategies to cope with high metal(loid)s concentrations in five mine isolates. The Clusters of Orthologous Group (COG) annotation showed that the “amino acid transport and metabolism” [E] was the most predominant functional category in all genomes. In addition, the KEEG pathways analysis also showed predicted genes for the biosynthetic pathways of most amino acids, indicating that amino acids could have an important role in the Ga leaching mechanism. The presence of effective resistance mechanisms to Ga and arsenic (As) was particularly important in GaAs bioleaching batch assays, and might explain the divergence in bioleaching efficiency among the bacterial strains. Rhodanobacter sp. B2A1Ga4 and Sphingomonas sp. A2-49 with higher resistance, mainly to As, were the most efficient bioleaching strains under these conditions. In bioleaching assays using cell-free spent medium Arthrobacter silviterrae A2-55 with lower As resistance outperformed all the other stains. Overall, higher efficiency in Ga leaching was obtained in bioleaching assays using GaAs when compared to GaN. Frontiers Media S.A. 2022-09-13 /pmc/articles/PMC9518604/ /pubmed/36188007 http://dx.doi.org/10.3389/fmicb.2022.970147 Text en Copyright © 2022 Chung, Francisco, Morais and Branco. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Chung, Ana Paula
Francisco, Romeu
Morais, Paula V.
Branco, Rita
Genome mining to unravel potential metabolic pathways linked to gallium bioleaching ability of bacterial mine isolates
title Genome mining to unravel potential metabolic pathways linked to gallium bioleaching ability of bacterial mine isolates
title_full Genome mining to unravel potential metabolic pathways linked to gallium bioleaching ability of bacterial mine isolates
title_fullStr Genome mining to unravel potential metabolic pathways linked to gallium bioleaching ability of bacterial mine isolates
title_full_unstemmed Genome mining to unravel potential metabolic pathways linked to gallium bioleaching ability of bacterial mine isolates
title_short Genome mining to unravel potential metabolic pathways linked to gallium bioleaching ability of bacterial mine isolates
title_sort genome mining to unravel potential metabolic pathways linked to gallium bioleaching ability of bacterial mine isolates
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9518604/
https://www.ncbi.nlm.nih.gov/pubmed/36188007
http://dx.doi.org/10.3389/fmicb.2022.970147
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