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A comparative study of interfacial environments in lipid nanodiscs and vesicles

Membrane protein conformations and dynamics are driven by the protein-lipid interactions occurring within the local environment of the membrane. These environments remain challenging to accurately capture in structural and biophysical experiments using bilayers. Consequently, there is an increasing...

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Autores principales: You, Xiao, Thakur, Naveen, Ray, Arka Prabha, Eddy, Matthew T., Baiz, Carlos R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9518727/
https://www.ncbi.nlm.nih.gov/pubmed/36176716
http://dx.doi.org/10.1016/j.bpr.2022.100066
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author You, Xiao
Thakur, Naveen
Ray, Arka Prabha
Eddy, Matthew T.
Baiz, Carlos R.
author_facet You, Xiao
Thakur, Naveen
Ray, Arka Prabha
Eddy, Matthew T.
Baiz, Carlos R.
author_sort You, Xiao
collection PubMed
description Membrane protein conformations and dynamics are driven by the protein-lipid interactions occurring within the local environment of the membrane. These environments remain challenging to accurately capture in structural and biophysical experiments using bilayers. Consequently, there is an increasing need for realistic cell-membrane mimetics for in vitro studies. Lipid nanodiscs provide certain advantages over vesicles for membrane protein studies. Nanodiscs are increasingly used for structural and spectroscopic characterization of membrane proteins. Despite the common use of nanodiscs, the interfacial environments of lipids confined to a ∼10-nm diameter area have remained relatively underexplored. Here, we use ultrafast two-dimensional infrared spectroscopy and temperature-dependent infrared absorption measurements of the ester carbonyls to compare the interfacial hydrogen bond structure and dynamics in lipid nanodiscs of varying lipid compositions and sizes with ∼100-nm vesicles. We examine the effects of lipid composition and nanodisc size. We found that nanodiscs and vesicles share largely similar lipid-water H-bond environments and interfacial dynamics. Differences in measured enthalpies of H-bonding suggest that H-bond dynamics in nanodiscs are modulated by the interaction between the annular lipids and the scaffold protein.
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spelling pubmed-95187272022-09-28 A comparative study of interfacial environments in lipid nanodiscs and vesicles You, Xiao Thakur, Naveen Ray, Arka Prabha Eddy, Matthew T. Baiz, Carlos R. Biophys Rep (N Y) Report Membrane protein conformations and dynamics are driven by the protein-lipid interactions occurring within the local environment of the membrane. These environments remain challenging to accurately capture in structural and biophysical experiments using bilayers. Consequently, there is an increasing need for realistic cell-membrane mimetics for in vitro studies. Lipid nanodiscs provide certain advantages over vesicles for membrane protein studies. Nanodiscs are increasingly used for structural and spectroscopic characterization of membrane proteins. Despite the common use of nanodiscs, the interfacial environments of lipids confined to a ∼10-nm diameter area have remained relatively underexplored. Here, we use ultrafast two-dimensional infrared spectroscopy and temperature-dependent infrared absorption measurements of the ester carbonyls to compare the interfacial hydrogen bond structure and dynamics in lipid nanodiscs of varying lipid compositions and sizes with ∼100-nm vesicles. We examine the effects of lipid composition and nanodisc size. We found that nanodiscs and vesicles share largely similar lipid-water H-bond environments and interfacial dynamics. Differences in measured enthalpies of H-bonding suggest that H-bond dynamics in nanodiscs are modulated by the interaction between the annular lipids and the scaffold protein. Elsevier 2022-07-22 /pmc/articles/PMC9518727/ /pubmed/36176716 http://dx.doi.org/10.1016/j.bpr.2022.100066 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Report
You, Xiao
Thakur, Naveen
Ray, Arka Prabha
Eddy, Matthew T.
Baiz, Carlos R.
A comparative study of interfacial environments in lipid nanodiscs and vesicles
title A comparative study of interfacial environments in lipid nanodiscs and vesicles
title_full A comparative study of interfacial environments in lipid nanodiscs and vesicles
title_fullStr A comparative study of interfacial environments in lipid nanodiscs and vesicles
title_full_unstemmed A comparative study of interfacial environments in lipid nanodiscs and vesicles
title_short A comparative study of interfacial environments in lipid nanodiscs and vesicles
title_sort comparative study of interfacial environments in lipid nanodiscs and vesicles
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9518727/
https://www.ncbi.nlm.nih.gov/pubmed/36176716
http://dx.doi.org/10.1016/j.bpr.2022.100066
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