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New microsatellite markers for the shallow coral Madracis auretenra from the Caribbean
Coral reefs species represent one-third of all marine species described in the ocean. They are also responsible for providing habitat and support to different species. Recently, Caribbean coral reefs ecosystems have suffered an alarming decrease in their populations as a result of overexploitation....
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9518922/ https://www.ncbi.nlm.nih.gov/pubmed/36170271 http://dx.doi.org/10.1371/journal.pone.0274895 |
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author | Ballesteros-Contreras, Diana Carolina Barrios, Lina Maria Preziosi, Richard |
author_facet | Ballesteros-Contreras, Diana Carolina Barrios, Lina Maria Preziosi, Richard |
author_sort | Ballesteros-Contreras, Diana Carolina |
collection | PubMed |
description | Coral reefs species represent one-third of all marine species described in the ocean. They are also responsible for providing habitat and support to different species. Recently, Caribbean coral reefs ecosystems have suffered an alarming decrease in their populations as a result of overexploitation. Madracis auretenra in particular, is a widespread shallow coral in marine protected areas (MPAs) of the Caribbean. Due to the important role of MPAs as a biodiversity conservation tool, this species can be used as a model to estimate its dispersion/migration among Caribbean reefs through the use of informative genetic markers (microsatellites) specifically designed for it. Seventeen new polymorphic microsatellites markers for M. auretenra were developed and tested in 330 samples from Colombia, Guatemala, Curacao and Barbados. The gene diversity (1-D) ranged from 0.482 to 0.903, while the evenness ranged from 0.456 to 0.884 after clone removal. The expected heterozygosity (He) ranged from 0.347 to 0.742, while the observed heterozygosity (Ho) ranged from 0.078 to 0.578. The specificity of our microsatellites shows the potential use of these markers in a-posteriori analysis to detect population structure at different spatial scales, where M. auretenra is reported. |
format | Online Article Text |
id | pubmed-9518922 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-95189222022-09-29 New microsatellite markers for the shallow coral Madracis auretenra from the Caribbean Ballesteros-Contreras, Diana Carolina Barrios, Lina Maria Preziosi, Richard PLoS One Research Article Coral reefs species represent one-third of all marine species described in the ocean. They are also responsible for providing habitat and support to different species. Recently, Caribbean coral reefs ecosystems have suffered an alarming decrease in their populations as a result of overexploitation. Madracis auretenra in particular, is a widespread shallow coral in marine protected areas (MPAs) of the Caribbean. Due to the important role of MPAs as a biodiversity conservation tool, this species can be used as a model to estimate its dispersion/migration among Caribbean reefs through the use of informative genetic markers (microsatellites) specifically designed for it. Seventeen new polymorphic microsatellites markers for M. auretenra were developed and tested in 330 samples from Colombia, Guatemala, Curacao and Barbados. The gene diversity (1-D) ranged from 0.482 to 0.903, while the evenness ranged from 0.456 to 0.884 after clone removal. The expected heterozygosity (He) ranged from 0.347 to 0.742, while the observed heterozygosity (Ho) ranged from 0.078 to 0.578. The specificity of our microsatellites shows the potential use of these markers in a-posteriori analysis to detect population structure at different spatial scales, where M. auretenra is reported. Public Library of Science 2022-09-28 /pmc/articles/PMC9518922/ /pubmed/36170271 http://dx.doi.org/10.1371/journal.pone.0274895 Text en © 2022 Ballesteros-Contreras et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Ballesteros-Contreras, Diana Carolina Barrios, Lina Maria Preziosi, Richard New microsatellite markers for the shallow coral Madracis auretenra from the Caribbean |
title | New microsatellite markers for the shallow coral Madracis auretenra from the Caribbean |
title_full | New microsatellite markers for the shallow coral Madracis auretenra from the Caribbean |
title_fullStr | New microsatellite markers for the shallow coral Madracis auretenra from the Caribbean |
title_full_unstemmed | New microsatellite markers for the shallow coral Madracis auretenra from the Caribbean |
title_short | New microsatellite markers for the shallow coral Madracis auretenra from the Caribbean |
title_sort | new microsatellite markers for the shallow coral madracis auretenra from the caribbean |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9518922/ https://www.ncbi.nlm.nih.gov/pubmed/36170271 http://dx.doi.org/10.1371/journal.pone.0274895 |
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