Cargando…

New microsatellite markers for the shallow coral Madracis auretenra from the Caribbean

Coral reefs species represent one-third of all marine species described in the ocean. They are also responsible for providing habitat and support to different species. Recently, Caribbean coral reefs ecosystems have suffered an alarming decrease in their populations as a result of overexploitation....

Descripción completa

Detalles Bibliográficos
Autores principales: Ballesteros-Contreras, Diana Carolina, Barrios, Lina Maria, Preziosi, Richard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9518922/
https://www.ncbi.nlm.nih.gov/pubmed/36170271
http://dx.doi.org/10.1371/journal.pone.0274895
_version_ 1784799292207136768
author Ballesteros-Contreras, Diana Carolina
Barrios, Lina Maria
Preziosi, Richard
author_facet Ballesteros-Contreras, Diana Carolina
Barrios, Lina Maria
Preziosi, Richard
author_sort Ballesteros-Contreras, Diana Carolina
collection PubMed
description Coral reefs species represent one-third of all marine species described in the ocean. They are also responsible for providing habitat and support to different species. Recently, Caribbean coral reefs ecosystems have suffered an alarming decrease in their populations as a result of overexploitation. Madracis auretenra in particular, is a widespread shallow coral in marine protected areas (MPAs) of the Caribbean. Due to the important role of MPAs as a biodiversity conservation tool, this species can be used as a model to estimate its dispersion/migration among Caribbean reefs through the use of informative genetic markers (microsatellites) specifically designed for it. Seventeen new polymorphic microsatellites markers for M. auretenra were developed and tested in 330 samples from Colombia, Guatemala, Curacao and Barbados. The gene diversity (1-D) ranged from 0.482 to 0.903, while the evenness ranged from 0.456 to 0.884 after clone removal. The expected heterozygosity (He) ranged from 0.347 to 0.742, while the observed heterozygosity (Ho) ranged from 0.078 to 0.578. The specificity of our microsatellites shows the potential use of these markers in a-posteriori analysis to detect population structure at different spatial scales, where M. auretenra is reported.
format Online
Article
Text
id pubmed-9518922
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-95189222022-09-29 New microsatellite markers for the shallow coral Madracis auretenra from the Caribbean Ballesteros-Contreras, Diana Carolina Barrios, Lina Maria Preziosi, Richard PLoS One Research Article Coral reefs species represent one-third of all marine species described in the ocean. They are also responsible for providing habitat and support to different species. Recently, Caribbean coral reefs ecosystems have suffered an alarming decrease in their populations as a result of overexploitation. Madracis auretenra in particular, is a widespread shallow coral in marine protected areas (MPAs) of the Caribbean. Due to the important role of MPAs as a biodiversity conservation tool, this species can be used as a model to estimate its dispersion/migration among Caribbean reefs through the use of informative genetic markers (microsatellites) specifically designed for it. Seventeen new polymorphic microsatellites markers for M. auretenra were developed and tested in 330 samples from Colombia, Guatemala, Curacao and Barbados. The gene diversity (1-D) ranged from 0.482 to 0.903, while the evenness ranged from 0.456 to 0.884 after clone removal. The expected heterozygosity (He) ranged from 0.347 to 0.742, while the observed heterozygosity (Ho) ranged from 0.078 to 0.578. The specificity of our microsatellites shows the potential use of these markers in a-posteriori analysis to detect population structure at different spatial scales, where M. auretenra is reported. Public Library of Science 2022-09-28 /pmc/articles/PMC9518922/ /pubmed/36170271 http://dx.doi.org/10.1371/journal.pone.0274895 Text en © 2022 Ballesteros-Contreras et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ballesteros-Contreras, Diana Carolina
Barrios, Lina Maria
Preziosi, Richard
New microsatellite markers for the shallow coral Madracis auretenra from the Caribbean
title New microsatellite markers for the shallow coral Madracis auretenra from the Caribbean
title_full New microsatellite markers for the shallow coral Madracis auretenra from the Caribbean
title_fullStr New microsatellite markers for the shallow coral Madracis auretenra from the Caribbean
title_full_unstemmed New microsatellite markers for the shallow coral Madracis auretenra from the Caribbean
title_short New microsatellite markers for the shallow coral Madracis auretenra from the Caribbean
title_sort new microsatellite markers for the shallow coral madracis auretenra from the caribbean
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9518922/
https://www.ncbi.nlm.nih.gov/pubmed/36170271
http://dx.doi.org/10.1371/journal.pone.0274895
work_keys_str_mv AT ballesteroscontrerasdianacarolina newmicrosatellitemarkersfortheshallowcoralmadracisauretenrafromthecaribbean
AT barrioslinamaria newmicrosatellitemarkersfortheshallowcoralmadracisauretenrafromthecaribbean
AT preziosirichard newmicrosatellitemarkersfortheshallowcoralmadracisauretenrafromthecaribbean