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Limosilactobacillus reuteri DS0384 promotes intestinal epithelial maturation via the postbiotic effect in human intestinal organoids and infant mice
Little is known about the modulatory capacity of the microbiota in early intestinal development. We examined various intestinal models that respond to gut microbial metabolites based on human pluripotent stem cell-derived human intestinal organoids (hIOs): physiologically relevant in vitro fetal-lik...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519030/ https://www.ncbi.nlm.nih.gov/pubmed/36130031 http://dx.doi.org/10.1080/19490976.2022.2121580 |
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author | Lee, Hana Jung, Kwang Bo Kwon, Ohman Son, Ye Seul Choi, Eunho Yu, Won Dong Son, Naeun Jeon, Jun Hyoung Jo, Hana Yang, Haneol Son, Yeong Rak Yun, Chan-Seok Cho, Hyun-Soo Kim, Sang Kyu Kim, Dae-Soo Park, Doo-Sang Son, Mi-Young |
author_facet | Lee, Hana Jung, Kwang Bo Kwon, Ohman Son, Ye Seul Choi, Eunho Yu, Won Dong Son, Naeun Jeon, Jun Hyoung Jo, Hana Yang, Haneol Son, Yeong Rak Yun, Chan-Seok Cho, Hyun-Soo Kim, Sang Kyu Kim, Dae-Soo Park, Doo-Sang Son, Mi-Young |
author_sort | Lee, Hana |
collection | PubMed |
description | Little is known about the modulatory capacity of the microbiota in early intestinal development. We examined various intestinal models that respond to gut microbial metabolites based on human pluripotent stem cell-derived human intestinal organoids (hIOs): physiologically relevant in vitro fetal-like intestine, intestinal stem cell, and intestinal disease models. We found that a newly isolated Limosilactobacillus reuteri strain DS0384 accelerated maturation of the fetal intestine using 3D hIO with immature fetal characteristics. Comparative metabolomic profiling analysis revealed that the secreted metabolite N-carbamyl glutamic acid (NCG) is involved in the beneficial effect of DS0384 cell-free supernatants on the intestinal maturation of hIOs. Experiments in an intestinal stem cell spheroid model and hIO-based intestinal inflamed model revealed that the cell-free supernatant from DS0384 comprising NCG promoted intestinal stem cell proliferation and was important for intestinal protection against cytokine-induced intestinal epithelial injury. The probiotic properties of DS0384 were also evaluated, including acid and bile tolerance and ability to adhere to human intestinal cells. Seven-day oral administration of DS0384 and cell-free supernatant promoted the intestinal development of newborn mice. Moreover, NCG exerted a protective effect on experimental colitis in mice. These results suggest that DS0384 is a useful agent for probiotic applications and therapeutic treatment for disorders of early gut development and for preventing intestinal barrier dysfunction. |
format | Online Article Text |
id | pubmed-9519030 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-95190302022-09-29 Limosilactobacillus reuteri DS0384 promotes intestinal epithelial maturation via the postbiotic effect in human intestinal organoids and infant mice Lee, Hana Jung, Kwang Bo Kwon, Ohman Son, Ye Seul Choi, Eunho Yu, Won Dong Son, Naeun Jeon, Jun Hyoung Jo, Hana Yang, Haneol Son, Yeong Rak Yun, Chan-Seok Cho, Hyun-Soo Kim, Sang Kyu Kim, Dae-Soo Park, Doo-Sang Son, Mi-Young Gut Microbes Research Paper Little is known about the modulatory capacity of the microbiota in early intestinal development. We examined various intestinal models that respond to gut microbial metabolites based on human pluripotent stem cell-derived human intestinal organoids (hIOs): physiologically relevant in vitro fetal-like intestine, intestinal stem cell, and intestinal disease models. We found that a newly isolated Limosilactobacillus reuteri strain DS0384 accelerated maturation of the fetal intestine using 3D hIO with immature fetal characteristics. Comparative metabolomic profiling analysis revealed that the secreted metabolite N-carbamyl glutamic acid (NCG) is involved in the beneficial effect of DS0384 cell-free supernatants on the intestinal maturation of hIOs. Experiments in an intestinal stem cell spheroid model and hIO-based intestinal inflamed model revealed that the cell-free supernatant from DS0384 comprising NCG promoted intestinal stem cell proliferation and was important for intestinal protection against cytokine-induced intestinal epithelial injury. The probiotic properties of DS0384 were also evaluated, including acid and bile tolerance and ability to adhere to human intestinal cells. Seven-day oral administration of DS0384 and cell-free supernatant promoted the intestinal development of newborn mice. Moreover, NCG exerted a protective effect on experimental colitis in mice. These results suggest that DS0384 is a useful agent for probiotic applications and therapeutic treatment for disorders of early gut development and for preventing intestinal barrier dysfunction. Taylor & Francis 2022-09-21 /pmc/articles/PMC9519030/ /pubmed/36130031 http://dx.doi.org/10.1080/19490976.2022.2121580 Text en © 2022 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Lee, Hana Jung, Kwang Bo Kwon, Ohman Son, Ye Seul Choi, Eunho Yu, Won Dong Son, Naeun Jeon, Jun Hyoung Jo, Hana Yang, Haneol Son, Yeong Rak Yun, Chan-Seok Cho, Hyun-Soo Kim, Sang Kyu Kim, Dae-Soo Park, Doo-Sang Son, Mi-Young Limosilactobacillus reuteri DS0384 promotes intestinal epithelial maturation via the postbiotic effect in human intestinal organoids and infant mice |
title | Limosilactobacillus reuteri DS0384 promotes intestinal epithelial maturation via the postbiotic effect in human intestinal organoids and infant mice |
title_full | Limosilactobacillus reuteri DS0384 promotes intestinal epithelial maturation via the postbiotic effect in human intestinal organoids and infant mice |
title_fullStr | Limosilactobacillus reuteri DS0384 promotes intestinal epithelial maturation via the postbiotic effect in human intestinal organoids and infant mice |
title_full_unstemmed | Limosilactobacillus reuteri DS0384 promotes intestinal epithelial maturation via the postbiotic effect in human intestinal organoids and infant mice |
title_short | Limosilactobacillus reuteri DS0384 promotes intestinal epithelial maturation via the postbiotic effect in human intestinal organoids and infant mice |
title_sort | limosilactobacillus reuteri ds0384 promotes intestinal epithelial maturation via the postbiotic effect in human intestinal organoids and infant mice |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519030/ https://www.ncbi.nlm.nih.gov/pubmed/36130031 http://dx.doi.org/10.1080/19490976.2022.2121580 |
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