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Excessive fructose intake inhibits skeletal development in adolescent rats via gut microbiota and energy metabolism

Excessive fructose intake from desserts and beverages may influence bone development among adolescents. The gut microbiota (GM) and energy metabolism play important roles in bone development. In this study, 40 female adolescent rats were randomly assigned to the control group, the fructose group wit...

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Autores principales: Gao, Tianlin, Tian, Chunyan, Tian, Ge, Ma, Li, Xu, Lili, Liu, Wendong, Cai, Jing, Zhong, Feng, Zhang, Huaqi, Ma, Aiguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519145/
https://www.ncbi.nlm.nih.gov/pubmed/36187951
http://dx.doi.org/10.3389/fmicb.2022.952892
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author Gao, Tianlin
Tian, Chunyan
Tian, Ge
Ma, Li
Xu, Lili
Liu, Wendong
Cai, Jing
Zhong, Feng
Zhang, Huaqi
Ma, Aiguo
author_facet Gao, Tianlin
Tian, Chunyan
Tian, Ge
Ma, Li
Xu, Lili
Liu, Wendong
Cai, Jing
Zhong, Feng
Zhang, Huaqi
Ma, Aiguo
author_sort Gao, Tianlin
collection PubMed
description Excessive fructose intake from desserts and beverages may influence bone development among adolescents. The gut microbiota (GM) and energy metabolism play important roles in bone development. In this study, 40 female adolescent rats were randomly assigned to the control group, the fructose group with two concentrations, and the glucose group as the positive control group. After 10 weeks, serum glucose and lipids were detected by means of an automatic analyzer, and the bone microstructure was analyzed by Micro-CT. Then, the GM was determined via 16S rRNA sequencing analysis, and energy metabolism was detected by measuring serum carbohydrate metabolites. At last, bone metabolism markers were measured via ELISA kits. The results showed that excessive fructose intake could increase body weight and influence the glucolipid metabolism of female adolescent rats. Meanwhile, the bone microstructures were impaired with excessive fructose intake. Mechanistically, excessive fructose intake shifted the GM of rats with the decrease of Lachnospiraceae, Ruminococcaceae, and increase of Allobaculum, Lachnospiraceae. Energy metabolism analysis suggested that most metabolites of fructose did not enter the tricarboxylic acid cycle to provide energy for the body’s development. Furthermore, serum bone metabolism markers showed that excessive fructose intake could decrease both bone formation and resorption. Our results suggested that excessive fructose intake could inhibit skeletal development in adolescents. One potential mechanism might be that it affected the intestinal microbiota homeostasis in the juvenile body, thus changing the energy metabolism level, and ultimately affecting the bone metabolic balance.
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spelling pubmed-95191452022-09-29 Excessive fructose intake inhibits skeletal development in adolescent rats via gut microbiota and energy metabolism Gao, Tianlin Tian, Chunyan Tian, Ge Ma, Li Xu, Lili Liu, Wendong Cai, Jing Zhong, Feng Zhang, Huaqi Ma, Aiguo Front Microbiol Microbiology Excessive fructose intake from desserts and beverages may influence bone development among adolescents. The gut microbiota (GM) and energy metabolism play important roles in bone development. In this study, 40 female adolescent rats were randomly assigned to the control group, the fructose group with two concentrations, and the glucose group as the positive control group. After 10 weeks, serum glucose and lipids were detected by means of an automatic analyzer, and the bone microstructure was analyzed by Micro-CT. Then, the GM was determined via 16S rRNA sequencing analysis, and energy metabolism was detected by measuring serum carbohydrate metabolites. At last, bone metabolism markers were measured via ELISA kits. The results showed that excessive fructose intake could increase body weight and influence the glucolipid metabolism of female adolescent rats. Meanwhile, the bone microstructures were impaired with excessive fructose intake. Mechanistically, excessive fructose intake shifted the GM of rats with the decrease of Lachnospiraceae, Ruminococcaceae, and increase of Allobaculum, Lachnospiraceae. Energy metabolism analysis suggested that most metabolites of fructose did not enter the tricarboxylic acid cycle to provide energy for the body’s development. Furthermore, serum bone metabolism markers showed that excessive fructose intake could decrease both bone formation and resorption. Our results suggested that excessive fructose intake could inhibit skeletal development in adolescents. One potential mechanism might be that it affected the intestinal microbiota homeostasis in the juvenile body, thus changing the energy metabolism level, and ultimately affecting the bone metabolic balance. Frontiers Media S.A. 2022-09-14 /pmc/articles/PMC9519145/ /pubmed/36187951 http://dx.doi.org/10.3389/fmicb.2022.952892 Text en Copyright © 2022 Gao, Tian, Tian, Ma, Xu, Liu, Cai, Zhong, Zhang and Ma. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Gao, Tianlin
Tian, Chunyan
Tian, Ge
Ma, Li
Xu, Lili
Liu, Wendong
Cai, Jing
Zhong, Feng
Zhang, Huaqi
Ma, Aiguo
Excessive fructose intake inhibits skeletal development in adolescent rats via gut microbiota and energy metabolism
title Excessive fructose intake inhibits skeletal development in adolescent rats via gut microbiota and energy metabolism
title_full Excessive fructose intake inhibits skeletal development in adolescent rats via gut microbiota and energy metabolism
title_fullStr Excessive fructose intake inhibits skeletal development in adolescent rats via gut microbiota and energy metabolism
title_full_unstemmed Excessive fructose intake inhibits skeletal development in adolescent rats via gut microbiota and energy metabolism
title_short Excessive fructose intake inhibits skeletal development in adolescent rats via gut microbiota and energy metabolism
title_sort excessive fructose intake inhibits skeletal development in adolescent rats via gut microbiota and energy metabolism
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519145/
https://www.ncbi.nlm.nih.gov/pubmed/36187951
http://dx.doi.org/10.3389/fmicb.2022.952892
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