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Laponite intercalated biomimetic multilayer coating prevents glucocorticoids induced orthopedic implant failure
Implant failure, which is commonly associated with failure of osseointegration and peri-implant infection, is a severe complication of orthopedic surgery. In particular, the survival rate of implants is significantly decreased in patients using long-term glucocorticoids (GCs). However, the exact mol...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519439/ https://www.ncbi.nlm.nih.gov/pubmed/36203962 http://dx.doi.org/10.1016/j.bioactmat.2022.09.013 |
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author | Liu, Zhe Tang, Qian Liu, Ruo-Tao Yu, Ming-Zhao Peng, Hao Zhang, Chang-Qing Zhu, Zhen-Zhong Wei, Xiao-Juan |
author_facet | Liu, Zhe Tang, Qian Liu, Ruo-Tao Yu, Ming-Zhao Peng, Hao Zhang, Chang-Qing Zhu, Zhen-Zhong Wei, Xiao-Juan |
author_sort | Liu, Zhe |
collection | PubMed |
description | Implant failure, which is commonly associated with failure of osseointegration and peri-implant infection, is a severe complication of orthopedic surgery. In particular, the survival rate of implants is significantly decreased in patients using long-term glucocorticoids (GCs). However, the exact molecular mechanism underlying GCs-induced implant loosening, as well as preventive strategies for these patients, is unclear. To address this problem, we performed RNA-sequencing and found that WNT16 was correlated with GCs-induced osteopenia (LogFC = −5.17, p < 0.01). Inspired by the concept of “organic-inorganic” hybrid, we theorized to introduce a bioactive two-dimensional nanosheet into a layer-by-layer (LbL) self-assembly coating to construct a customized implant that targets WNT16. After screening commercially available nanosheets, laponite (LAP) was identified as a cost-effective rescuer for GCs-induced WNT16 inhibition, which was then intercalated into LbL deposition system consisting of quaternized chitosan (QCS) and hyaluronic acid (HA). The hybrid coating (QCS/HA/LAP) showed micrometer thickness and improved hydrophilicity and interface roughness. Furthermore, QCS/HA/LAP coated polyetheretherketone (PEEK) implant enhanced cell viability, adhesion, and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), and promoted osteointegration of PEEK in GCs-treated rats by targeting the WNT16/β-catenin axis. The assembled QCS has proven antibacterial properties, and the hybrid coating exerted potent detrimental effects against methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli), both in vitro and in vivo. Taken together, these results suggest that QCS/HA/LAP coating has great potential for use in implants customization, and has synergistic pro-osteogenic and antibacterial effects that help prevent implant failure in GCs-treated patients. |
format | Online Article Text |
id | pubmed-9519439 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | KeAi Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-95194392022-10-05 Laponite intercalated biomimetic multilayer coating prevents glucocorticoids induced orthopedic implant failure Liu, Zhe Tang, Qian Liu, Ruo-Tao Yu, Ming-Zhao Peng, Hao Zhang, Chang-Qing Zhu, Zhen-Zhong Wei, Xiao-Juan Bioact Mater Article Implant failure, which is commonly associated with failure of osseointegration and peri-implant infection, is a severe complication of orthopedic surgery. In particular, the survival rate of implants is significantly decreased in patients using long-term glucocorticoids (GCs). However, the exact molecular mechanism underlying GCs-induced implant loosening, as well as preventive strategies for these patients, is unclear. To address this problem, we performed RNA-sequencing and found that WNT16 was correlated with GCs-induced osteopenia (LogFC = −5.17, p < 0.01). Inspired by the concept of “organic-inorganic” hybrid, we theorized to introduce a bioactive two-dimensional nanosheet into a layer-by-layer (LbL) self-assembly coating to construct a customized implant that targets WNT16. After screening commercially available nanosheets, laponite (LAP) was identified as a cost-effective rescuer for GCs-induced WNT16 inhibition, which was then intercalated into LbL deposition system consisting of quaternized chitosan (QCS) and hyaluronic acid (HA). The hybrid coating (QCS/HA/LAP) showed micrometer thickness and improved hydrophilicity and interface roughness. Furthermore, QCS/HA/LAP coated polyetheretherketone (PEEK) implant enhanced cell viability, adhesion, and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), and promoted osteointegration of PEEK in GCs-treated rats by targeting the WNT16/β-catenin axis. The assembled QCS has proven antibacterial properties, and the hybrid coating exerted potent detrimental effects against methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli), both in vitro and in vivo. Taken together, these results suggest that QCS/HA/LAP coating has great potential for use in implants customization, and has synergistic pro-osteogenic and antibacterial effects that help prevent implant failure in GCs-treated patients. KeAi Publishing 2022-09-26 /pmc/articles/PMC9519439/ /pubmed/36203962 http://dx.doi.org/10.1016/j.bioactmat.2022.09.013 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Liu, Zhe Tang, Qian Liu, Ruo-Tao Yu, Ming-Zhao Peng, Hao Zhang, Chang-Qing Zhu, Zhen-Zhong Wei, Xiao-Juan Laponite intercalated biomimetic multilayer coating prevents glucocorticoids induced orthopedic implant failure |
title | Laponite intercalated biomimetic multilayer coating prevents glucocorticoids induced orthopedic implant failure |
title_full | Laponite intercalated biomimetic multilayer coating prevents glucocorticoids induced orthopedic implant failure |
title_fullStr | Laponite intercalated biomimetic multilayer coating prevents glucocorticoids induced orthopedic implant failure |
title_full_unstemmed | Laponite intercalated biomimetic multilayer coating prevents glucocorticoids induced orthopedic implant failure |
title_short | Laponite intercalated biomimetic multilayer coating prevents glucocorticoids induced orthopedic implant failure |
title_sort | laponite intercalated biomimetic multilayer coating prevents glucocorticoids induced orthopedic implant failure |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519439/ https://www.ncbi.nlm.nih.gov/pubmed/36203962 http://dx.doi.org/10.1016/j.bioactmat.2022.09.013 |
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