Phos-tag-based non-radioactive protocols for monitoring Arabidopsis kinase activities in vitro

Kinases are indispensable signaling components. Radioactive-based phosphorylation assays are widely used but require specific protective equipment and safety trainings. Here, we present a Phos-tag-based non-radioactive kinase assay to study Arabidopsis kinase activities. We expressed and purified bo...

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Detalles Bibliográficos
Autores principales: Shi, Junjie, Fang, Manru, Wang, Ran, Zhu, Ziqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519594/
https://www.ncbi.nlm.nih.gov/pubmed/36149791
http://dx.doi.org/10.1016/j.xpro.2022.101717

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519594/
https://www.ncbi.nlm.nih.gov/pubmed/36149791
http://dx.doi.org/10.1016/j.xpro.2022.101717

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