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Phos-tag-based non-radioactive protocols for monitoring Arabidopsis kinase activities in vitro
Kinases are indispensable signaling components. Radioactive-based phosphorylation assays are widely used but require specific protective equipment and safety trainings. Here, we present a Phos-tag-based non-radioactive kinase assay to study Arabidopsis kinase activities. We expressed and purified bo...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519594/ https://www.ncbi.nlm.nih.gov/pubmed/36149791 http://dx.doi.org/10.1016/j.xpro.2022.101717 |