Assembly of plant virus agroinfectious clones using biological material or DNA synthesis

Infectious clone technology is universally applied for biological characterization and engineering of viruses. This protocol describes procedures that implement synthetic biology advances for streamlined assembly of virus infectious clones. Here, I detail homology-based cloning using biological mate...

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Autor principal: Pasin, Fabio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519601/
https://www.ncbi.nlm.nih.gov/pubmed/36149792
http://dx.doi.org/10.1016/j.xpro.2022.101716
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author Pasin, Fabio
author_facet Pasin, Fabio
author_sort Pasin, Fabio
collection PubMed
description Infectious clone technology is universally applied for biological characterization and engineering of viruses. This protocol describes procedures that implement synthetic biology advances for streamlined assembly of virus infectious clones. Here, I detail homology-based cloning using biological material, as well as SynViP assembly using type IIS restriction enzymes and chemically synthesized DNA fragments. The assembled virus clones are based on compact T-DNA binary vectors of the pLX series and are delivered to host plants by Agrobacterium-mediated inoculation. For complete details on the use and execution of this protocol, please refer to Pasin et al. (2017, 2018) and Pasin (2021).
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spelling pubmed-95196012022-09-30 Assembly of plant virus agroinfectious clones using biological material or DNA synthesis Pasin, Fabio STAR Protoc Protocol Infectious clone technology is universally applied for biological characterization and engineering of viruses. This protocol describes procedures that implement synthetic biology advances for streamlined assembly of virus infectious clones. Here, I detail homology-based cloning using biological material, as well as SynViP assembly using type IIS restriction enzymes and chemically synthesized DNA fragments. The assembled virus clones are based on compact T-DNA binary vectors of the pLX series and are delivered to host plants by Agrobacterium-mediated inoculation. For complete details on the use and execution of this protocol, please refer to Pasin et al. (2017, 2018) and Pasin (2021). Elsevier 2022-09-22 /pmc/articles/PMC9519601/ /pubmed/36149792 http://dx.doi.org/10.1016/j.xpro.2022.101716 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Pasin, Fabio
Assembly of plant virus agroinfectious clones using biological material or DNA synthesis
title Assembly of plant virus agroinfectious clones using biological material or DNA synthesis
title_full Assembly of plant virus agroinfectious clones using biological material or DNA synthesis
title_fullStr Assembly of plant virus agroinfectious clones using biological material or DNA synthesis
title_full_unstemmed Assembly of plant virus agroinfectious clones using biological material or DNA synthesis
title_short Assembly of plant virus agroinfectious clones using biological material or DNA synthesis
title_sort assembly of plant virus agroinfectious clones using biological material or dna synthesis
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519601/
https://www.ncbi.nlm.nih.gov/pubmed/36149792
http://dx.doi.org/10.1016/j.xpro.2022.101716
work_keys_str_mv AT pasinfabio assemblyofplantvirusagroinfectiousclonesusingbiologicalmaterialordnasynthesis

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