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Assembly of plant virus agroinfectious clones using biological material or DNA synthesis
Infectious clone technology is universally applied for biological characterization and engineering of viruses. This protocol describes procedures that implement synthetic biology advances for streamlined assembly of virus infectious clones. Here, I detail homology-based cloning using biological mate...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Elsevier
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519601/ https://www.ncbi.nlm.nih.gov/pubmed/36149792 http://dx.doi.org/10.1016/j.xpro.2022.101716 |