Cargando…
The replacement of bacitracin methylene disalicylate with Bacillus subtilis PB6 in the diet of male Cherry Valley Ducks reduces the feed conversion ratio by improving intestinal health and modulating gut microbiota
In this study, we compared the impacts of Bacillus subtilis PB6 (BS) and bacitracin methylene disalicylate (BMD) on the growth performance, intestinal morphology, expression of tight connection protein, and cecal microbiota community of male ducks through a 42-d trial. Three-hundred and sixty male C...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519614/ https://www.ncbi.nlm.nih.gov/pubmed/36155883 http://dx.doi.org/10.1016/j.psj.2022.102155 |
_version_ | 1784799439212249088 |
---|---|
author | Zhang, Qianqian Li, Jian Wang, Guixiang Wang, Lizhi Zhang, Zhiming Fang, Zhengfeng Lin, Yan Xu, Shengyu Feng, Bin Zhuo, Yong Hua, Lun Jiang, Xuemei Zhao, Xilun Wu, De Che, Lianqiang |
author_facet | Zhang, Qianqian Li, Jian Wang, Guixiang Wang, Lizhi Zhang, Zhiming Fang, Zhengfeng Lin, Yan Xu, Shengyu Feng, Bin Zhuo, Yong Hua, Lun Jiang, Xuemei Zhao, Xilun Wu, De Che, Lianqiang |
author_sort | Zhang, Qianqian |
collection | PubMed |
description | In this study, we compared the impacts of Bacillus subtilis PB6 (BS) and bacitracin methylene disalicylate (BMD) on the growth performance, intestinal morphology, expression of tight connection protein, and cecal microbiota community of male ducks through a 42-d trial. Three-hundred and sixty male Cherry Valley meat-type ducklings (1-day-old) were distributed into 3 groups of 6 replicates: CON group (control, basal diet), BMD group (basal diet + 45 mg/kg BMD, active ingredient dose in the feed), and BS group (basal diet + 2 × 10(7) CFU/kg BS in the feed). Results showed that supplementing the diet with BS reduced the average daily feed intake (ADFI) during d 15 to 42 and d 1 to 42 compared with the CON group (P = 0.032). It also reduced feed conversion ratio (FCR) during d 15 to 42 and d 1 to 42 (P < 0.05) relative to the other groups. The ileal villus height (VH) and villus height /crypt depth ratio (V/C) were increased (P < 0.05) in both the BS and BMD groups, and the jejunal VH and V/C ratio were increased in the BS group (P < 0.05). Relative to the CON, BS supplementation was associated with numerical augmentation of goblet cells in the jejunal mucosa and upregulation of jejunal zonula occludens (ZO-1) and ileal mucin2 (P < 0.05) mRNA levels. Analysis showed a negative correlation between FCR (d 0–42) and VH, V/C, and the number of goblet cells in the jejunum (P < 0.05). Additionally, BMD or BS supplementation altered the alpha diversity of colonic microbiota (P < 0.05). Correlation analysis revealed that Butyricimonas, Enterobacteriaceae, Clostridiaceae, and Tannerellaceae were positively associated with the acetic acid and butyrate concentrations (P < 0.05). Taken together, the supplementation of BS in the diet of male ducks was conducive to reducing FCR by meliorating intestinal morphology, upregulating ZO-1 and mucin2 mRNA levels, regulating the abundance of microbiota, and metabolites, and having a greater effect than BMD supplementation. |
format | Online Article Text |
id | pubmed-9519614 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-95196142022-09-30 The replacement of bacitracin methylene disalicylate with Bacillus subtilis PB6 in the diet of male Cherry Valley Ducks reduces the feed conversion ratio by improving intestinal health and modulating gut microbiota Zhang, Qianqian Li, Jian Wang, Guixiang Wang, Lizhi Zhang, Zhiming Fang, Zhengfeng Lin, Yan Xu, Shengyu Feng, Bin Zhuo, Yong Hua, Lun Jiang, Xuemei Zhao, Xilun Wu, De Che, Lianqiang Poult Sci METABOLISM AND NUTRITION In this study, we compared the impacts of Bacillus subtilis PB6 (BS) and bacitracin methylene disalicylate (BMD) on the growth performance, intestinal morphology, expression of tight connection protein, and cecal microbiota community of male ducks through a 42-d trial. Three-hundred and sixty male Cherry Valley meat-type ducklings (1-day-old) were distributed into 3 groups of 6 replicates: CON group (control, basal diet), BMD group (basal diet + 45 mg/kg BMD, active ingredient dose in the feed), and BS group (basal diet + 2 × 10(7) CFU/kg BS in the feed). Results showed that supplementing the diet with BS reduced the average daily feed intake (ADFI) during d 15 to 42 and d 1 to 42 compared with the CON group (P = 0.032). It also reduced feed conversion ratio (FCR) during d 15 to 42 and d 1 to 42 (P < 0.05) relative to the other groups. The ileal villus height (VH) and villus height /crypt depth ratio (V/C) were increased (P < 0.05) in both the BS and BMD groups, and the jejunal VH and V/C ratio were increased in the BS group (P < 0.05). Relative to the CON, BS supplementation was associated with numerical augmentation of goblet cells in the jejunal mucosa and upregulation of jejunal zonula occludens (ZO-1) and ileal mucin2 (P < 0.05) mRNA levels. Analysis showed a negative correlation between FCR (d 0–42) and VH, V/C, and the number of goblet cells in the jejunum (P < 0.05). Additionally, BMD or BS supplementation altered the alpha diversity of colonic microbiota (P < 0.05). Correlation analysis revealed that Butyricimonas, Enterobacteriaceae, Clostridiaceae, and Tannerellaceae were positively associated with the acetic acid and butyrate concentrations (P < 0.05). Taken together, the supplementation of BS in the diet of male ducks was conducive to reducing FCR by meliorating intestinal morphology, upregulating ZO-1 and mucin2 mRNA levels, regulating the abundance of microbiota, and metabolites, and having a greater effect than BMD supplementation. Elsevier 2022-08-27 /pmc/articles/PMC9519614/ /pubmed/36155883 http://dx.doi.org/10.1016/j.psj.2022.102155 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | METABOLISM AND NUTRITION Zhang, Qianqian Li, Jian Wang, Guixiang Wang, Lizhi Zhang, Zhiming Fang, Zhengfeng Lin, Yan Xu, Shengyu Feng, Bin Zhuo, Yong Hua, Lun Jiang, Xuemei Zhao, Xilun Wu, De Che, Lianqiang The replacement of bacitracin methylene disalicylate with Bacillus subtilis PB6 in the diet of male Cherry Valley Ducks reduces the feed conversion ratio by improving intestinal health and modulating gut microbiota |
title | The replacement of bacitracin methylene disalicylate with Bacillus subtilis PB6 in the diet of male Cherry Valley Ducks reduces the feed conversion ratio by improving intestinal health and modulating gut microbiota |
title_full | The replacement of bacitracin methylene disalicylate with Bacillus subtilis PB6 in the diet of male Cherry Valley Ducks reduces the feed conversion ratio by improving intestinal health and modulating gut microbiota |
title_fullStr | The replacement of bacitracin methylene disalicylate with Bacillus subtilis PB6 in the diet of male Cherry Valley Ducks reduces the feed conversion ratio by improving intestinal health and modulating gut microbiota |
title_full_unstemmed | The replacement of bacitracin methylene disalicylate with Bacillus subtilis PB6 in the diet of male Cherry Valley Ducks reduces the feed conversion ratio by improving intestinal health and modulating gut microbiota |
title_short | The replacement of bacitracin methylene disalicylate with Bacillus subtilis PB6 in the diet of male Cherry Valley Ducks reduces the feed conversion ratio by improving intestinal health and modulating gut microbiota |
title_sort | replacement of bacitracin methylene disalicylate with bacillus subtilis pb6 in the diet of male cherry valley ducks reduces the feed conversion ratio by improving intestinal health and modulating gut microbiota |
topic | METABOLISM AND NUTRITION |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519614/ https://www.ncbi.nlm.nih.gov/pubmed/36155883 http://dx.doi.org/10.1016/j.psj.2022.102155 |
work_keys_str_mv | AT zhangqianqian thereplacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT lijian thereplacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT wangguixiang thereplacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT wanglizhi thereplacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT zhangzhiming thereplacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT fangzhengfeng thereplacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT linyan thereplacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT xushengyu thereplacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT fengbin thereplacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT zhuoyong thereplacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT hualun thereplacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT jiangxuemei thereplacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT zhaoxilun thereplacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT wude thereplacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT chelianqiang thereplacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT zhangqianqian replacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT lijian replacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT wangguixiang replacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT wanglizhi replacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT zhangzhiming replacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT fangzhengfeng replacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT linyan replacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT xushengyu replacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT fengbin replacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT zhuoyong replacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT hualun replacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT jiangxuemei replacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT zhaoxilun replacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT wude replacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota AT chelianqiang replacementofbacitracinmethylenedisalicylatewithbacillussubtilispb6inthedietofmalecherryvalleyducksreducesthefeedconversionratiobyimprovingintestinalhealthandmodulatinggutmicrobiota |