Isotopic tracing of glucose metabolites in human monocytes to assess changes in inflammatory conditions

Differences in metabolic profiles can link to functional changes of immune cells in disease conditions. Here, we detail a protocol for the detection and quantitation of 19 metabolites in one analytical run. We provide the parameters for chromatographic separation and mass spectrometric analysis of i...

Descripción completa

Detalles Bibliográficos
Autores principales: Giacomello, Ginevra, Böttcher, Chotima, Parr, Maria Kristina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519621/
https://www.ncbi.nlm.nih.gov/pubmed/36152304
http://dx.doi.org/10.1016/j.xpro.2022.101715
_version_ 1784799440960225280
author Giacomello, Ginevra
Böttcher, Chotima
Parr, Maria Kristina
author_facet Giacomello, Ginevra
Böttcher, Chotima
Parr, Maria Kristina
author_sort Giacomello, Ginevra
collection PubMed
description Differences in metabolic profiles can link to functional changes of immune cells in disease conditions. Here, we detail a protocol for the detection and quantitation of 19 metabolites in one analytical run. We provide the parameters for chromatographic separation and mass spectrometric analysis of isotopically labeled and unlabeled metabolites. We include steps for incubation and sample preparation of PBMCs and monocytes. This protocol overcomes the chromatographic challenges caused by the chelating properties of some metabolites.
format Online
Article
Text
id pubmed-9519621
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-95196212022-09-30 Isotopic tracing of glucose metabolites in human monocytes to assess changes in inflammatory conditions Giacomello, Ginevra Böttcher, Chotima Parr, Maria Kristina STAR Protoc Protocol Differences in metabolic profiles can link to functional changes of immune cells in disease conditions. Here, we detail a protocol for the detection and quantitation of 19 metabolites in one analytical run. We provide the parameters for chromatographic separation and mass spectrometric analysis of isotopically labeled and unlabeled metabolites. We include steps for incubation and sample preparation of PBMCs and monocytes. This protocol overcomes the chromatographic challenges caused by the chelating properties of some metabolites. Elsevier 2022-09-23 /pmc/articles/PMC9519621/ /pubmed/36152304 http://dx.doi.org/10.1016/j.xpro.2022.101715 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Giacomello, Ginevra
Böttcher, Chotima
Parr, Maria Kristina
Isotopic tracing of glucose metabolites in human monocytes to assess changes in inflammatory conditions
title Isotopic tracing of glucose metabolites in human monocytes to assess changes in inflammatory conditions
title_full Isotopic tracing of glucose metabolites in human monocytes to assess changes in inflammatory conditions
title_fullStr Isotopic tracing of glucose metabolites in human monocytes to assess changes in inflammatory conditions
title_full_unstemmed Isotopic tracing of glucose metabolites in human monocytes to assess changes in inflammatory conditions
title_short Isotopic tracing of glucose metabolites in human monocytes to assess changes in inflammatory conditions
title_sort isotopic tracing of glucose metabolites in human monocytes to assess changes in inflammatory conditions
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519621/
https://www.ncbi.nlm.nih.gov/pubmed/36152304
http://dx.doi.org/10.1016/j.xpro.2022.101715
work_keys_str_mv AT giacomelloginevra isotopictracingofglucosemetabolitesinhumanmonocytestoassesschangesininflammatoryconditions
AT bottcherchotima isotopictracingofglucosemetabolitesinhumanmonocytestoassesschangesininflammatoryconditions
AT parrmariakristina isotopictracingofglucosemetabolitesinhumanmonocytestoassesschangesininflammatoryconditions

Ejemplares similares