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Isotopic tracing of glucose metabolites in human monocytes to assess changes in inflammatory conditions
Differences in metabolic profiles can link to functional changes of immune cells in disease conditions. Here, we detail a protocol for the detection and quantitation of 19 metabolites in one analytical run. We provide the parameters for chromatographic separation and mass spectrometric analysis of i...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519621/ https://www.ncbi.nlm.nih.gov/pubmed/36152304 http://dx.doi.org/10.1016/j.xpro.2022.101715 |