Multiplex immunofluorescence-guided laser capture microdissection for spatial transcriptomics of metastatic melanoma tissues

We describe a pipeline for optimized and streamlined multiplexed immunofluorescence-guided laser capture microdissection allowing the harvest of individual cells based on their phenotype and tissue localization for transcriptomic analysis with next-generation RNA sequencing. Here, we analyze transcr...

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Detalles Bibliográficos
Autores principales: Martinek, Jan, Wu, Te-Chia, Sun, Lili, Lin, Jianan, Kim, Kyung In, Marches, Florentina, Robson, Paul, George, Joshy, Palucka, Karolina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519630/
https://www.ncbi.nlm.nih.gov/pubmed/36149794
http://dx.doi.org/10.1016/j.xpro.2022.101698

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