Cargando…

In vitro grafting of hepatic spheroids and organoids on a microfluidic vascular bed

With recent progress in modeling liver organogenesis and regeneration, the lack of vasculature is becoming the bottleneck in progressing our ability to model human hepatic tissues in vitro. Here, we introduce a platform for routine grafting of liver and other tissues on an in vitro grown microvascul...

Descripción completa

Detalles Bibliográficos
Autores principales: Bonanini, Flavio, Kurek, Dorota, Previdi, Sara, Nicolas, Arnaud, Hendriks, Delilah, de Ruiter, Sander, Meyer, Marine, Clapés Cabrer, Maria, Dinkelberg, Roelof, García, Silvia Bonilla, Kramer, Bart, Olivier, Thomas, Hu, Huili, López-Iglesias, Carmen, Schavemaker, Frederik, Walinga, Erik, Dutta, Devanjali, Queiroz, Karla, Domansky, Karel, Ronden, Bob, Joore, Jos, Lanz, Henriette L., Peters, Peter J., Trietsch, Sebastiaan J., Clevers, Hans, Vulto, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519670/
https://www.ncbi.nlm.nih.gov/pubmed/35704148
http://dx.doi.org/10.1007/s10456-022-09842-9
_version_ 1784799451836055552
author Bonanini, Flavio
Kurek, Dorota
Previdi, Sara
Nicolas, Arnaud
Hendriks, Delilah
de Ruiter, Sander
Meyer, Marine
Clapés Cabrer, Maria
Dinkelberg, Roelof
García, Silvia Bonilla
Kramer, Bart
Olivier, Thomas
Hu, Huili
López-Iglesias, Carmen
Schavemaker, Frederik
Walinga, Erik
Dutta, Devanjali
Queiroz, Karla
Domansky, Karel
Ronden, Bob
Joore, Jos
Lanz, Henriette L.
Peters, Peter J.
Trietsch, Sebastiaan J.
Clevers, Hans
Vulto, Paul
author_facet Bonanini, Flavio
Kurek, Dorota
Previdi, Sara
Nicolas, Arnaud
Hendriks, Delilah
de Ruiter, Sander
Meyer, Marine
Clapés Cabrer, Maria
Dinkelberg, Roelof
García, Silvia Bonilla
Kramer, Bart
Olivier, Thomas
Hu, Huili
López-Iglesias, Carmen
Schavemaker, Frederik
Walinga, Erik
Dutta, Devanjali
Queiroz, Karla
Domansky, Karel
Ronden, Bob
Joore, Jos
Lanz, Henriette L.
Peters, Peter J.
Trietsch, Sebastiaan J.
Clevers, Hans
Vulto, Paul
author_sort Bonanini, Flavio
collection PubMed
description With recent progress in modeling liver organogenesis and regeneration, the lack of vasculature is becoming the bottleneck in progressing our ability to model human hepatic tissues in vitro. Here, we introduce a platform for routine grafting of liver and other tissues on an in vitro grown microvascular bed. The platform consists of 64 microfluidic chips patterned underneath a 384-well microtiter plate. Each chip allows the formation of a microvascular bed between two main lateral vessels by inducing angiogenesis. Chips consist of an open-top microfluidic chamber, which enables addition of a target tissue by manual or robotic pipetting. Upon grafting a liver microtissue, the microvascular bed undergoes anastomosis, resulting in a stable, perfusable vascular network. Interactions with vasculature were found in spheroids and organoids upon 7 days of co-culture with space of Disse-like architecture in between hepatocytes and endothelium. Veno-occlusive disease was induced by azathioprine exposure, leading to impeded perfusion of the vascularized spheroid. The platform holds the potential to replace animals with an in vitro alternative for routine grafting of spheroids, organoids, or (patient-derived) explants. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10456-022-09842-9.
format Online
Article
Text
id pubmed-9519670
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Springer Netherlands
record_format MEDLINE/PubMed
spelling pubmed-95196702022-09-30 In vitro grafting of hepatic spheroids and organoids on a microfluidic vascular bed Bonanini, Flavio Kurek, Dorota Previdi, Sara Nicolas, Arnaud Hendriks, Delilah de Ruiter, Sander Meyer, Marine Clapés Cabrer, Maria Dinkelberg, Roelof García, Silvia Bonilla Kramer, Bart Olivier, Thomas Hu, Huili López-Iglesias, Carmen Schavemaker, Frederik Walinga, Erik Dutta, Devanjali Queiroz, Karla Domansky, Karel Ronden, Bob Joore, Jos Lanz, Henriette L. Peters, Peter J. Trietsch, Sebastiaan J. Clevers, Hans Vulto, Paul Angiogenesis Original Paper With recent progress in modeling liver organogenesis and regeneration, the lack of vasculature is becoming the bottleneck in progressing our ability to model human hepatic tissues in vitro. Here, we introduce a platform for routine grafting of liver and other tissues on an in vitro grown microvascular bed. The platform consists of 64 microfluidic chips patterned underneath a 384-well microtiter plate. Each chip allows the formation of a microvascular bed between two main lateral vessels by inducing angiogenesis. Chips consist of an open-top microfluidic chamber, which enables addition of a target tissue by manual or robotic pipetting. Upon grafting a liver microtissue, the microvascular bed undergoes anastomosis, resulting in a stable, perfusable vascular network. Interactions with vasculature were found in spheroids and organoids upon 7 days of co-culture with space of Disse-like architecture in between hepatocytes and endothelium. Veno-occlusive disease was induced by azathioprine exposure, leading to impeded perfusion of the vascularized spheroid. The platform holds the potential to replace animals with an in vitro alternative for routine grafting of spheroids, organoids, or (patient-derived) explants. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10456-022-09842-9. Springer Netherlands 2022-06-15 2022 /pmc/articles/PMC9519670/ /pubmed/35704148 http://dx.doi.org/10.1007/s10456-022-09842-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Paper
Bonanini, Flavio
Kurek, Dorota
Previdi, Sara
Nicolas, Arnaud
Hendriks, Delilah
de Ruiter, Sander
Meyer, Marine
Clapés Cabrer, Maria
Dinkelberg, Roelof
García, Silvia Bonilla
Kramer, Bart
Olivier, Thomas
Hu, Huili
López-Iglesias, Carmen
Schavemaker, Frederik
Walinga, Erik
Dutta, Devanjali
Queiroz, Karla
Domansky, Karel
Ronden, Bob
Joore, Jos
Lanz, Henriette L.
Peters, Peter J.
Trietsch, Sebastiaan J.
Clevers, Hans
Vulto, Paul
In vitro grafting of hepatic spheroids and organoids on a microfluidic vascular bed
title In vitro grafting of hepatic spheroids and organoids on a microfluidic vascular bed
title_full In vitro grafting of hepatic spheroids and organoids on a microfluidic vascular bed
title_fullStr In vitro grafting of hepatic spheroids and organoids on a microfluidic vascular bed
title_full_unstemmed In vitro grafting of hepatic spheroids and organoids on a microfluidic vascular bed
title_short In vitro grafting of hepatic spheroids and organoids on a microfluidic vascular bed
title_sort in vitro grafting of hepatic spheroids and organoids on a microfluidic vascular bed
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519670/
https://www.ncbi.nlm.nih.gov/pubmed/35704148
http://dx.doi.org/10.1007/s10456-022-09842-9
work_keys_str_mv AT bonaniniflavio invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT kurekdorota invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT previdisara invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT nicolasarnaud invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT hendriksdelilah invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT deruitersander invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT meyermarine invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT clapescabrermaria invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT dinkelbergroelof invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT garciasilviabonilla invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT kramerbart invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT olivierthomas invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT huhuili invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT lopeziglesiascarmen invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT schavemakerfrederik invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT walingaerik invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT duttadevanjali invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT queirozkarla invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT domanskykarel invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT rondenbob invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT joorejos invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT lanzhenriettel invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT peterspeterj invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT trietschsebastiaanj invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT clevershans invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed
AT vultopaul invitrograftingofhepaticspheroidsandorganoidsonamicrofluidicvascularbed