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Effect of caffeine on genes expressions of developing retinas in the chick model

It has been reported that overconsumption of caffeine during pregnancy leads to a deleterious effect within the nervous tissues during embryonic development. In this study, we further extrapolated the effect of caffeine in the developing retinas, which is known to be one of the most sensitive tissue...

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Autores principales: Lekchaoum, Thanyarat, Buddawong, Aticha, Ahi, Sunalin, Chandee, Nichapha, Weerachatyanukul, Wattana, Asuvapongpatana, Somluk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Association of Anatomists 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519763/
https://www.ncbi.nlm.nih.gov/pubmed/35918321
http://dx.doi.org/10.5115/acb.22.034
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author Lekchaoum, Thanyarat
Buddawong, Aticha
Ahi, Sunalin
Chandee, Nichapha
Weerachatyanukul, Wattana
Asuvapongpatana, Somluk
author_facet Lekchaoum, Thanyarat
Buddawong, Aticha
Ahi, Sunalin
Chandee, Nichapha
Weerachatyanukul, Wattana
Asuvapongpatana, Somluk
author_sort Lekchaoum, Thanyarat
collection PubMed
description It has been reported that overconsumption of caffeine during pregnancy leads to a deleterious effect within the nervous tissues during embryonic development. In this study, we further extrapolated the effect of caffeine in the developing retinas, which is known to be one of the most sensitive tissues in chick embryos. Morphological changes of retinal thickness and organization of neuroretinal epithelium were monitored using three gene markers, Atoh7, FoxN4, and Lim1. Upon treating with a single dose of caffeine (15 µmol at embryonic day 1 [E1]), relative thicknesses of developing retinas (particularly of E7 and E9) were significantly altered. Among the three genes studied, the expression pattern of Atoh7 was notably altered while those of FoxN4, and Lim1 mRNA showed only a slight change in these developing retinas. Quantitative polymerase chain reaction results supported the most notable changes of Atoh7 but not FoxN4, and Lim1 gene in the developing retinas, particularly at E7. The effect of caffeine towards other organs during development should be extrapolated and the awareness of its intensive consumption should be raised.
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spelling pubmed-95197632022-10-08 Effect of caffeine on genes expressions of developing retinas in the chick model Lekchaoum, Thanyarat Buddawong, Aticha Ahi, Sunalin Chandee, Nichapha Weerachatyanukul, Wattana Asuvapongpatana, Somluk Anat Cell Biol Original Article It has been reported that overconsumption of caffeine during pregnancy leads to a deleterious effect within the nervous tissues during embryonic development. In this study, we further extrapolated the effect of caffeine in the developing retinas, which is known to be one of the most sensitive tissues in chick embryos. Morphological changes of retinal thickness and organization of neuroretinal epithelium were monitored using three gene markers, Atoh7, FoxN4, and Lim1. Upon treating with a single dose of caffeine (15 µmol at embryonic day 1 [E1]), relative thicknesses of developing retinas (particularly of E7 and E9) were significantly altered. Among the three genes studied, the expression pattern of Atoh7 was notably altered while those of FoxN4, and Lim1 mRNA showed only a slight change in these developing retinas. Quantitative polymerase chain reaction results supported the most notable changes of Atoh7 but not FoxN4, and Lim1 gene in the developing retinas, particularly at E7. The effect of caffeine towards other organs during development should be extrapolated and the awareness of its intensive consumption should be raised. Korean Association of Anatomists 2022-09-30 2022-09-30 /pmc/articles/PMC9519763/ /pubmed/35918321 http://dx.doi.org/10.5115/acb.22.034 Text en Copyright © 2022. Anatomy & Cell Biology https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Lekchaoum, Thanyarat
Buddawong, Aticha
Ahi, Sunalin
Chandee, Nichapha
Weerachatyanukul, Wattana
Asuvapongpatana, Somluk
Effect of caffeine on genes expressions of developing retinas in the chick model
title Effect of caffeine on genes expressions of developing retinas in the chick model
title_full Effect of caffeine on genes expressions of developing retinas in the chick model
title_fullStr Effect of caffeine on genes expressions of developing retinas in the chick model
title_full_unstemmed Effect of caffeine on genes expressions of developing retinas in the chick model
title_short Effect of caffeine on genes expressions of developing retinas in the chick model
title_sort effect of caffeine on genes expressions of developing retinas in the chick model
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519763/
https://www.ncbi.nlm.nih.gov/pubmed/35918321
http://dx.doi.org/10.5115/acb.22.034
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