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DIA label-free proteomic analysis of murine bone-marrow-derived macrophages

Here, we describe an optimized protocol to analyze murine bone-marrow-derived macrophages using label-free data-independent acquisition (DIA) proteomics. We provide a complete step-by-step protocol describing sample preparation utilizing the S-Trap approach for on-column digestion and peptide purifi...

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Detalles Bibliográficos
Autores principales:	Baker, Christa P., Phair, Iain R., Brenes, Alejandro J., Atrih, Abdelmadjid, Ryan, Dylan G., Bruderer, Roland, Dinkova-Kostova, Albena T., Lamont, Douglas J., Arthur, J. Simon C., Howden, Andrew J.M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519785/ https://www.ncbi.nlm.nih.gov/pubmed/36166358 http://dx.doi.org/10.1016/j.xpro.2022.101725

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