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The use of mucoadhesive oral patches containing epigallocatechin-3-gallate to treat periodontitis: an in vivo study
OBJECTIVES: The application of topical drugs such as mucoadhesive oral patches (MOPs) do not irritate the mucosa and are able to increase the permeability of drugs to oral tissue. Epigallocatechin-3-gallate (EGCG) is an active ingredient that exhibits significant antibacterial and anti-inflammatory...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taibah University
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519795/ https://www.ncbi.nlm.nih.gov/pubmed/36212598 http://dx.doi.org/10.1016/j.jtumed.2022.06.006 |
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author | Lashari, Dur Muhammad Aljunaid, Mohammed Lashari, Yasmeen Qaid, Huda Rashad Ridwan, Rini Devijanti Diyatri, Indeswati Kaid, Nejva Alkadasi, Baleegh Abdulraoof |
author_facet | Lashari, Dur Muhammad Aljunaid, Mohammed Lashari, Yasmeen Qaid, Huda Rashad Ridwan, Rini Devijanti Diyatri, Indeswati Kaid, Nejva Alkadasi, Baleegh Abdulraoof |
author_sort | Lashari, Dur Muhammad |
collection | PubMed |
description | OBJECTIVES: The application of topical drugs such as mucoadhesive oral patches (MOPs) do not irritate the mucosa and are able to increase the permeability of drugs to oral tissue. Epigallocatechin-3-gallate (EGCG) is an active ingredient that exhibits significant antibacterial and anti-inflammatory effects. The purpose of this study was to analyze the therapeutic potential of a mucoadhesive oral patch containing EGCG (MOP-EGCG) in a model of periodontitis and investigate its effects on the expression of osteoprotegerin (OPG), receptor activator of nuclear factor-kappa Β ligand (RANKL) and receptor activator of nuclear factor-κB (RANK). METHODS: A model of periodontitis was induced in Rattus novergicus used Porphyromonas gingivalis by applying 0.03 ml of bacteria locally with 1 × 10(10) colony-forming units (CFU) seven times at 2-day intervals in the central lower incisors. Periodontitis was then treated with MOP (control), a mucoadhesive oral patch containing doxycycline (MOP-doxy) or MOP-EGCG for 1 h/day for 21 days. On days 3, 5, 7, 14 and 21 after treatment, the central lower incisor was biopsied and analyzed by immunohistochemistry for RANK/RANKL and OPG expression in the gingiva tissue. RESULTS: MOP-EGCG extract significantly reduced the expression of RANKL and increased the expression of OPG and RANK (p < 0.05) when compared to the MOP-doxy and MOP groups. CONCLUSION: MOP-EGCG extract reduced the expression of RANKL and increased the expression of OPG and RANK, thus suggesting that MOP-EGCG can inhibit the loss of alveolar bone in periodontitis. |
format | Online Article Text |
id | pubmed-9519795 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Taibah University |
record_format | MEDLINE/PubMed |
spelling | pubmed-95197952022-10-06 The use of mucoadhesive oral patches containing epigallocatechin-3-gallate to treat periodontitis: an in vivo study Lashari, Dur Muhammad Aljunaid, Mohammed Lashari, Yasmeen Qaid, Huda Rashad Ridwan, Rini Devijanti Diyatri, Indeswati Kaid, Nejva Alkadasi, Baleegh Abdulraoof J Taibah Univ Med Sci Original Article OBJECTIVES: The application of topical drugs such as mucoadhesive oral patches (MOPs) do not irritate the mucosa and are able to increase the permeability of drugs to oral tissue. Epigallocatechin-3-gallate (EGCG) is an active ingredient that exhibits significant antibacterial and anti-inflammatory effects. The purpose of this study was to analyze the therapeutic potential of a mucoadhesive oral patch containing EGCG (MOP-EGCG) in a model of periodontitis and investigate its effects on the expression of osteoprotegerin (OPG), receptor activator of nuclear factor-kappa Β ligand (RANKL) and receptor activator of nuclear factor-κB (RANK). METHODS: A model of periodontitis was induced in Rattus novergicus used Porphyromonas gingivalis by applying 0.03 ml of bacteria locally with 1 × 10(10) colony-forming units (CFU) seven times at 2-day intervals in the central lower incisors. Periodontitis was then treated with MOP (control), a mucoadhesive oral patch containing doxycycline (MOP-doxy) or MOP-EGCG for 1 h/day for 21 days. On days 3, 5, 7, 14 and 21 after treatment, the central lower incisor was biopsied and analyzed by immunohistochemistry for RANK/RANKL and OPG expression in the gingiva tissue. RESULTS: MOP-EGCG extract significantly reduced the expression of RANKL and increased the expression of OPG and RANK (p < 0.05) when compared to the MOP-doxy and MOP groups. CONCLUSION: MOP-EGCG extract reduced the expression of RANKL and increased the expression of OPG and RANK, thus suggesting that MOP-EGCG can inhibit the loss of alveolar bone in periodontitis. Taibah University 2022-07-07 /pmc/articles/PMC9519795/ /pubmed/36212598 http://dx.doi.org/10.1016/j.jtumed.2022.06.006 Text en © 2022 [The Author/The Authors] https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Original Article Lashari, Dur Muhammad Aljunaid, Mohammed Lashari, Yasmeen Qaid, Huda Rashad Ridwan, Rini Devijanti Diyatri, Indeswati Kaid, Nejva Alkadasi, Baleegh Abdulraoof The use of mucoadhesive oral patches containing epigallocatechin-3-gallate to treat periodontitis: an in vivo study |
title | The use of mucoadhesive oral patches containing epigallocatechin-3-gallate to treat periodontitis: an in vivo study |
title_full | The use of mucoadhesive oral patches containing epigallocatechin-3-gallate to treat periodontitis: an in vivo study |
title_fullStr | The use of mucoadhesive oral patches containing epigallocatechin-3-gallate to treat periodontitis: an in vivo study |
title_full_unstemmed | The use of mucoadhesive oral patches containing epigallocatechin-3-gallate to treat periodontitis: an in vivo study |
title_short | The use of mucoadhesive oral patches containing epigallocatechin-3-gallate to treat periodontitis: an in vivo study |
title_sort | use of mucoadhesive oral patches containing epigallocatechin-3-gallate to treat periodontitis: an in vivo study |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519795/ https://www.ncbi.nlm.nih.gov/pubmed/36212598 http://dx.doi.org/10.1016/j.jtumed.2022.06.006 |
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