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In-depth insights into cervicovaginal microbial communities and hrHPV infections using high-resolution microbiome profiling
The cervicovaginal microbiome (CVM) correlates with women’s cervical health, and variations in its composition are associated with high-risk human papillomavirus (hrHPV) infection outcomes. Cervicovaginal microbes have been grouped into five community state types (CSTs) based on microbial community...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519886/ https://www.ncbi.nlm.nih.gov/pubmed/36171433 http://dx.doi.org/10.1038/s41522-022-00336-6 |
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author | Molina, Mariano A. Andralojc, Karolina M. Huynen, Martijn A. Leenders, William P. J. Melchers, Willem J. G. |
author_facet | Molina, Mariano A. Andralojc, Karolina M. Huynen, Martijn A. Leenders, William P. J. Melchers, Willem J. G. |
author_sort | Molina, Mariano A. |
collection | PubMed |
description | The cervicovaginal microbiome (CVM) correlates with women’s cervical health, and variations in its composition are associated with high-risk human papillomavirus (hrHPV) infection outcomes. Cervicovaginal microbes have been grouped into five community state types (CSTs) based on microbial community composition and abundance. However, studying the impact of CSTs in health and disease is challenging because the current sequencing technologies have limited confident discrimination between closely related and yet functionally different bacterial species. Circular probe-based RNA sequencing (ciRNAseq) achieves high-resolution microbiome profiling and therefore provides in-depth and unambiguous knowledge about the composition of the CVM. Based on ciRNAseq profiling of a large cohort of cervical smears (n = 541), we here define subgroups of CSTs I, III, and IV based on intra-CST differences with respect to abundances of Lactobacillus acidophilus (CSTs I-A vs. I-B and CSTs III-A vs. III-B), Lactobacillus iners (CSTs I-A vs. I-B and CSTs III-A vs. III-B), and Megasphaera genomosp type 1 (CSTs IV-A vs. IV-B). Our results further support the existence of subgroups of CST IV-C that are dominant for non-Lactobacillus species and have intermediate microbial diversity. We also show that CST V is associated with uninfected conditions, and CST IV-A associates with hrHPV-induced cervical disease. In conclusion, we characterized new subdivisions of cervicovaginal CSTs, which may further advance our understanding of women’s cervical health and hrHPV-related progression to disease. |
format | Online Article Text |
id | pubmed-9519886 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-95198862022-09-30 In-depth insights into cervicovaginal microbial communities and hrHPV infections using high-resolution microbiome profiling Molina, Mariano A. Andralojc, Karolina M. Huynen, Martijn A. Leenders, William P. J. Melchers, Willem J. G. NPJ Biofilms Microbiomes Article The cervicovaginal microbiome (CVM) correlates with women’s cervical health, and variations in its composition are associated with high-risk human papillomavirus (hrHPV) infection outcomes. Cervicovaginal microbes have been grouped into five community state types (CSTs) based on microbial community composition and abundance. However, studying the impact of CSTs in health and disease is challenging because the current sequencing technologies have limited confident discrimination between closely related and yet functionally different bacterial species. Circular probe-based RNA sequencing (ciRNAseq) achieves high-resolution microbiome profiling and therefore provides in-depth and unambiguous knowledge about the composition of the CVM. Based on ciRNAseq profiling of a large cohort of cervical smears (n = 541), we here define subgroups of CSTs I, III, and IV based on intra-CST differences with respect to abundances of Lactobacillus acidophilus (CSTs I-A vs. I-B and CSTs III-A vs. III-B), Lactobacillus iners (CSTs I-A vs. I-B and CSTs III-A vs. III-B), and Megasphaera genomosp type 1 (CSTs IV-A vs. IV-B). Our results further support the existence of subgroups of CST IV-C that are dominant for non-Lactobacillus species and have intermediate microbial diversity. We also show that CST V is associated with uninfected conditions, and CST IV-A associates with hrHPV-induced cervical disease. In conclusion, we characterized new subdivisions of cervicovaginal CSTs, which may further advance our understanding of women’s cervical health and hrHPV-related progression to disease. Nature Publishing Group UK 2022-09-28 /pmc/articles/PMC9519886/ /pubmed/36171433 http://dx.doi.org/10.1038/s41522-022-00336-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Molina, Mariano A. Andralojc, Karolina M. Huynen, Martijn A. Leenders, William P. J. Melchers, Willem J. G. In-depth insights into cervicovaginal microbial communities and hrHPV infections using high-resolution microbiome profiling |
title | In-depth insights into cervicovaginal microbial communities and hrHPV infections using high-resolution microbiome profiling |
title_full | In-depth insights into cervicovaginal microbial communities and hrHPV infections using high-resolution microbiome profiling |
title_fullStr | In-depth insights into cervicovaginal microbial communities and hrHPV infections using high-resolution microbiome profiling |
title_full_unstemmed | In-depth insights into cervicovaginal microbial communities and hrHPV infections using high-resolution microbiome profiling |
title_short | In-depth insights into cervicovaginal microbial communities and hrHPV infections using high-resolution microbiome profiling |
title_sort | in-depth insights into cervicovaginal microbial communities and hrhpv infections using high-resolution microbiome profiling |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519886/ https://www.ncbi.nlm.nih.gov/pubmed/36171433 http://dx.doi.org/10.1038/s41522-022-00336-6 |
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