Cargando…
Screening of interferon-stimulated genes against avian reovirus infection and mechanistic exploration of the antiviral activity of IFIT5
Avian reovirus (ARV) infection can lead to severe immunosuppression, complications, and secondary diseases, causing immense economic losses to the poultry industry. In-depth study of the mechanism by which the innate immune system combats ARV infection, especially the antiviral effect mediated by in...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9520478/ https://www.ncbi.nlm.nih.gov/pubmed/36187980 http://dx.doi.org/10.3389/fmicb.2022.998505 |
_version_ | 1784799633276403712 |
---|---|
author | Wang, Sheng Wan, Lijun Ren, Hongyu Xie, Zhixun Xie, Liji Huang, Jiaoling Deng, Xianwen Xie, Zhiqin Luo, Sisi Li, Meng Zeng, Tingting Zhang, Yanfang Zhang, Minxiu |
author_facet | Wang, Sheng Wan, Lijun Ren, Hongyu Xie, Zhixun Xie, Liji Huang, Jiaoling Deng, Xianwen Xie, Zhiqin Luo, Sisi Li, Meng Zeng, Tingting Zhang, Yanfang Zhang, Minxiu |
author_sort | Wang, Sheng |
collection | PubMed |
description | Avian reovirus (ARV) infection can lead to severe immunosuppression, complications, and secondary diseases, causing immense economic losses to the poultry industry. In-depth study of the mechanism by which the innate immune system combats ARV infection, especially the antiviral effect mediated by interferon, is needed to prevent and contain ARV infection. In this study, ARV strain S1133 was used to artificially infect 7-day-old specific pathogen–free chickens. The results indicated that ARV rapidly proliferated in the immune organs, including the spleen, bursa of Fabricius, and thymus. The viral load peaked early in the infection and led to varying degrees of pathological damage to tissues and organs. Real-time quantitative PCR revealed that the mRNA levels of interferon and multiple interferon-stimulated genes (ISGs) in the spleen, bursa of Fabricius, and thymus were upregulated to varying degrees in the early stage of infection. Among the ISGs, IFIT5, and Mx were the most upregulated in various tissues and organs, suggesting that they are important ISGs for host resistance to ARV infection. Further investigation of the role of IFIT5 in ARV infection showed that overexpression of the IFIT5 gene inhibited ARV replication, whereas inhibition of the endogenously expressed IFIT5 gene by siRNA promoted ARV replication. IFIT5 may be a positive feedback regulator of the innate immune signaling pathways during ARV infection and may induce IFN-α production by promoting the expression of MAD5 and MAVS to exert its antiviral effect. The results of this study help explain the innate immune regulatory mechanism of ARV infection and reveal the important role of IFIT5 in inhibiting ARV replication, which has important theoretical significance and practical application value for the prevention and control of ARV infection. |
format | Online Article Text |
id | pubmed-9520478 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95204782022-09-30 Screening of interferon-stimulated genes against avian reovirus infection and mechanistic exploration of the antiviral activity of IFIT5 Wang, Sheng Wan, Lijun Ren, Hongyu Xie, Zhixun Xie, Liji Huang, Jiaoling Deng, Xianwen Xie, Zhiqin Luo, Sisi Li, Meng Zeng, Tingting Zhang, Yanfang Zhang, Minxiu Front Microbiol Microbiology Avian reovirus (ARV) infection can lead to severe immunosuppression, complications, and secondary diseases, causing immense economic losses to the poultry industry. In-depth study of the mechanism by which the innate immune system combats ARV infection, especially the antiviral effect mediated by interferon, is needed to prevent and contain ARV infection. In this study, ARV strain S1133 was used to artificially infect 7-day-old specific pathogen–free chickens. The results indicated that ARV rapidly proliferated in the immune organs, including the spleen, bursa of Fabricius, and thymus. The viral load peaked early in the infection and led to varying degrees of pathological damage to tissues and organs. Real-time quantitative PCR revealed that the mRNA levels of interferon and multiple interferon-stimulated genes (ISGs) in the spleen, bursa of Fabricius, and thymus were upregulated to varying degrees in the early stage of infection. Among the ISGs, IFIT5, and Mx were the most upregulated in various tissues and organs, suggesting that they are important ISGs for host resistance to ARV infection. Further investigation of the role of IFIT5 in ARV infection showed that overexpression of the IFIT5 gene inhibited ARV replication, whereas inhibition of the endogenously expressed IFIT5 gene by siRNA promoted ARV replication. IFIT5 may be a positive feedback regulator of the innate immune signaling pathways during ARV infection and may induce IFN-α production by promoting the expression of MAD5 and MAVS to exert its antiviral effect. The results of this study help explain the innate immune regulatory mechanism of ARV infection and reveal the important role of IFIT5 in inhibiting ARV replication, which has important theoretical significance and practical application value for the prevention and control of ARV infection. Frontiers Media S.A. 2022-09-15 /pmc/articles/PMC9520478/ /pubmed/36187980 http://dx.doi.org/10.3389/fmicb.2022.998505 Text en Copyright © 2022 Wang, Wan, Ren, Xie, Xie, Huang, Deng, Xie, Luo, Li, Zeng, Zhang and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Wang, Sheng Wan, Lijun Ren, Hongyu Xie, Zhixun Xie, Liji Huang, Jiaoling Deng, Xianwen Xie, Zhiqin Luo, Sisi Li, Meng Zeng, Tingting Zhang, Yanfang Zhang, Minxiu Screening of interferon-stimulated genes against avian reovirus infection and mechanistic exploration of the antiviral activity of IFIT5 |
title | Screening of interferon-stimulated genes against avian reovirus infection and mechanistic exploration of the antiviral activity of IFIT5 |
title_full | Screening of interferon-stimulated genes against avian reovirus infection and mechanistic exploration of the antiviral activity of IFIT5 |
title_fullStr | Screening of interferon-stimulated genes against avian reovirus infection and mechanistic exploration of the antiviral activity of IFIT5 |
title_full_unstemmed | Screening of interferon-stimulated genes against avian reovirus infection and mechanistic exploration of the antiviral activity of IFIT5 |
title_short | Screening of interferon-stimulated genes against avian reovirus infection and mechanistic exploration of the antiviral activity of IFIT5 |
title_sort | screening of interferon-stimulated genes against avian reovirus infection and mechanistic exploration of the antiviral activity of ifit5 |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9520478/ https://www.ncbi.nlm.nih.gov/pubmed/36187980 http://dx.doi.org/10.3389/fmicb.2022.998505 |
work_keys_str_mv | AT wangsheng screeningofinterferonstimulatedgenesagainstavianreovirusinfectionandmechanisticexplorationoftheantiviralactivityofifit5 AT wanlijun screeningofinterferonstimulatedgenesagainstavianreovirusinfectionandmechanisticexplorationoftheantiviralactivityofifit5 AT renhongyu screeningofinterferonstimulatedgenesagainstavianreovirusinfectionandmechanisticexplorationoftheantiviralactivityofifit5 AT xiezhixun screeningofinterferonstimulatedgenesagainstavianreovirusinfectionandmechanisticexplorationoftheantiviralactivityofifit5 AT xieliji screeningofinterferonstimulatedgenesagainstavianreovirusinfectionandmechanisticexplorationoftheantiviralactivityofifit5 AT huangjiaoling screeningofinterferonstimulatedgenesagainstavianreovirusinfectionandmechanisticexplorationoftheantiviralactivityofifit5 AT dengxianwen screeningofinterferonstimulatedgenesagainstavianreovirusinfectionandmechanisticexplorationoftheantiviralactivityofifit5 AT xiezhiqin screeningofinterferonstimulatedgenesagainstavianreovirusinfectionandmechanisticexplorationoftheantiviralactivityofifit5 AT luosisi screeningofinterferonstimulatedgenesagainstavianreovirusinfectionandmechanisticexplorationoftheantiviralactivityofifit5 AT limeng screeningofinterferonstimulatedgenesagainstavianreovirusinfectionandmechanisticexplorationoftheantiviralactivityofifit5 AT zengtingting screeningofinterferonstimulatedgenesagainstavianreovirusinfectionandmechanisticexplorationoftheantiviralactivityofifit5 AT zhangyanfang screeningofinterferonstimulatedgenesagainstavianreovirusinfectionandmechanisticexplorationoftheantiviralactivityofifit5 AT zhangminxiu screeningofinterferonstimulatedgenesagainstavianreovirusinfectionandmechanisticexplorationoftheantiviralactivityofifit5 |