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Screening of interferon-stimulated genes against avian reovirus infection and mechanistic exploration of the antiviral activity of IFIT5

Avian reovirus (ARV) infection can lead to severe immunosuppression, complications, and secondary diseases, causing immense economic losses to the poultry industry. In-depth study of the mechanism by which the innate immune system combats ARV infection, especially the antiviral effect mediated by in...

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Autores principales: Wang, Sheng, Wan, Lijun, Ren, Hongyu, Xie, Zhixun, Xie, Liji, Huang, Jiaoling, Deng, Xianwen, Xie, Zhiqin, Luo, Sisi, Li, Meng, Zeng, Tingting, Zhang, Yanfang, Zhang, Minxiu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9520478/
https://www.ncbi.nlm.nih.gov/pubmed/36187980
http://dx.doi.org/10.3389/fmicb.2022.998505
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author Wang, Sheng
Wan, Lijun
Ren, Hongyu
Xie, Zhixun
Xie, Liji
Huang, Jiaoling
Deng, Xianwen
Xie, Zhiqin
Luo, Sisi
Li, Meng
Zeng, Tingting
Zhang, Yanfang
Zhang, Minxiu
author_facet Wang, Sheng
Wan, Lijun
Ren, Hongyu
Xie, Zhixun
Xie, Liji
Huang, Jiaoling
Deng, Xianwen
Xie, Zhiqin
Luo, Sisi
Li, Meng
Zeng, Tingting
Zhang, Yanfang
Zhang, Minxiu
author_sort Wang, Sheng
collection PubMed
description Avian reovirus (ARV) infection can lead to severe immunosuppression, complications, and secondary diseases, causing immense economic losses to the poultry industry. In-depth study of the mechanism by which the innate immune system combats ARV infection, especially the antiviral effect mediated by interferon, is needed to prevent and contain ARV infection. In this study, ARV strain S1133 was used to artificially infect 7-day-old specific pathogen–free chickens. The results indicated that ARV rapidly proliferated in the immune organs, including the spleen, bursa of Fabricius, and thymus. The viral load peaked early in the infection and led to varying degrees of pathological damage to tissues and organs. Real-time quantitative PCR revealed that the mRNA levels of interferon and multiple interferon-stimulated genes (ISGs) in the spleen, bursa of Fabricius, and thymus were upregulated to varying degrees in the early stage of infection. Among the ISGs, IFIT5, and Mx were the most upregulated in various tissues and organs, suggesting that they are important ISGs for host resistance to ARV infection. Further investigation of the role of IFIT5 in ARV infection showed that overexpression of the IFIT5 gene inhibited ARV replication, whereas inhibition of the endogenously expressed IFIT5 gene by siRNA promoted ARV replication. IFIT5 may be a positive feedback regulator of the innate immune signaling pathways during ARV infection and may induce IFN-α production by promoting the expression of MAD5 and MAVS to exert its antiviral effect. The results of this study help explain the innate immune regulatory mechanism of ARV infection and reveal the important role of IFIT5 in inhibiting ARV replication, which has important theoretical significance and practical application value for the prevention and control of ARV infection.
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spelling pubmed-95204782022-09-30 Screening of interferon-stimulated genes against avian reovirus infection and mechanistic exploration of the antiviral activity of IFIT5 Wang, Sheng Wan, Lijun Ren, Hongyu Xie, Zhixun Xie, Liji Huang, Jiaoling Deng, Xianwen Xie, Zhiqin Luo, Sisi Li, Meng Zeng, Tingting Zhang, Yanfang Zhang, Minxiu Front Microbiol Microbiology Avian reovirus (ARV) infection can lead to severe immunosuppression, complications, and secondary diseases, causing immense economic losses to the poultry industry. In-depth study of the mechanism by which the innate immune system combats ARV infection, especially the antiviral effect mediated by interferon, is needed to prevent and contain ARV infection. In this study, ARV strain S1133 was used to artificially infect 7-day-old specific pathogen–free chickens. The results indicated that ARV rapidly proliferated in the immune organs, including the spleen, bursa of Fabricius, and thymus. The viral load peaked early in the infection and led to varying degrees of pathological damage to tissues and organs. Real-time quantitative PCR revealed that the mRNA levels of interferon and multiple interferon-stimulated genes (ISGs) in the spleen, bursa of Fabricius, and thymus were upregulated to varying degrees in the early stage of infection. Among the ISGs, IFIT5, and Mx were the most upregulated in various tissues and organs, suggesting that they are important ISGs for host resistance to ARV infection. Further investigation of the role of IFIT5 in ARV infection showed that overexpression of the IFIT5 gene inhibited ARV replication, whereas inhibition of the endogenously expressed IFIT5 gene by siRNA promoted ARV replication. IFIT5 may be a positive feedback regulator of the innate immune signaling pathways during ARV infection and may induce IFN-α production by promoting the expression of MAD5 and MAVS to exert its antiviral effect. The results of this study help explain the innate immune regulatory mechanism of ARV infection and reveal the important role of IFIT5 in inhibiting ARV replication, which has important theoretical significance and practical application value for the prevention and control of ARV infection. Frontiers Media S.A. 2022-09-15 /pmc/articles/PMC9520478/ /pubmed/36187980 http://dx.doi.org/10.3389/fmicb.2022.998505 Text en Copyright © 2022 Wang, Wan, Ren, Xie, Xie, Huang, Deng, Xie, Luo, Li, Zeng, Zhang and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wang, Sheng
Wan, Lijun
Ren, Hongyu
Xie, Zhixun
Xie, Liji
Huang, Jiaoling
Deng, Xianwen
Xie, Zhiqin
Luo, Sisi
Li, Meng
Zeng, Tingting
Zhang, Yanfang
Zhang, Minxiu
Screening of interferon-stimulated genes against avian reovirus infection and mechanistic exploration of the antiviral activity of IFIT5
title Screening of interferon-stimulated genes against avian reovirus infection and mechanistic exploration of the antiviral activity of IFIT5
title_full Screening of interferon-stimulated genes against avian reovirus infection and mechanistic exploration of the antiviral activity of IFIT5
title_fullStr Screening of interferon-stimulated genes against avian reovirus infection and mechanistic exploration of the antiviral activity of IFIT5
title_full_unstemmed Screening of interferon-stimulated genes against avian reovirus infection and mechanistic exploration of the antiviral activity of IFIT5
title_short Screening of interferon-stimulated genes against avian reovirus infection and mechanistic exploration of the antiviral activity of IFIT5
title_sort screening of interferon-stimulated genes against avian reovirus infection and mechanistic exploration of the antiviral activity of ifit5
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9520478/
https://www.ncbi.nlm.nih.gov/pubmed/36187980
http://dx.doi.org/10.3389/fmicb.2022.998505
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