Efficient and reversible Cas13d-mediated knockdown with an all-in-one lentivirus-vector

Type VI CRISPR effector Cas13d from Ruminococcus flavefaciens XPD3002 (RfxCas13d) is an RNA-guided RNA endonuclease. RfxCas13d has been harnessed to knockdown gene expression with high specificity in various systems including mammalian cells. While inducible knockdown is advantageous over constituti...

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Detalles Bibliográficos
Autores principales: Lv, Suli, Zhao, Xuefeng, Ma, Xianyun, Zou, Qingli, Li, Neng, Yan, Yingying, Sun, Lidong, Song, Tanjing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9521038/
https://www.ncbi.nlm.nih.gov/pubmed/36185457
http://dx.doi.org/10.3389/fbioe.2022.960192

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9521038/
https://www.ncbi.nlm.nih.gov/pubmed/36185457
http://dx.doi.org/10.3389/fbioe.2022.960192

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