Cargando…

Comparative Evaluation of the Antibacterial Efficacy of Chlorhexidine and 810 nm Diode Laser in the Disinfection of Root Canals Contaminated With Enterococcus faecalis: An In Vitro Study

Introduction: This study compared the antibacterial efficacy of three different root canal irrigants, sodium hypochlorite, chlorhexidine and 810 nm diode laser and diode laser in combination with chlorhexidine in root canals contaminated with Enterococcus faecalis. Methods: Fifty extracted mandibula...

Descripción completa

Detalles Bibliográficos
Autores principales: Mathew, Teris, BM, Shanthala, GV, Prashanth, Jose, Jubin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cureus 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9521298/
https://www.ncbi.nlm.nih.gov/pubmed/36185833
http://dx.doi.org/10.7759/cureus.28596
_version_ 1784799806591336448
author Mathew, Teris
BM, Shanthala
GV, Prashanth
Jose, Jubin
author_facet Mathew, Teris
BM, Shanthala
GV, Prashanth
Jose, Jubin
author_sort Mathew, Teris
collection PubMed
description Introduction: This study compared the antibacterial efficacy of three different root canal irrigants, sodium hypochlorite, chlorhexidine and 810 nm diode laser and diode laser in combination with chlorhexidine in root canals contaminated with Enterococcus faecalis. Methods: Fifty extracted mandibular first premolars were decoronated at the cementoenamel junction and cut at the apical end; biomechanical preparation was done, autoclaved and contaminated with Enterococcus faecalis. The specimens were divided into five groups containing 10 teeth each: group I: saline (negative control), group II: 2.5% sodium hypochlorite, group III: 2% chlorhexidine gluconate solution, group IV: diode laser, and group V: diode laser in combination with 2% chlorhexidine. Disinfection was carried out, after which dentinal shavings were collected from the specimens of each group. Antimicrobial efficacy was tested by counting the colony-forming units of viable Enterococcus faecalis on the agar plates. One-way ANOVA and Scheffe’s post hoc test were done to analyse the results. Results: Diode laser with chlorhexidine group showed minimum colony-forming units followed by the diode laser group. The post hoc test showed a statistically significant difference between saline, 2.5% sodium hypochlorite and 2% chlorhexidine groups (P = 0.001) and a non-significant difference between diode laser and diode laser with chlorhexidine group (P = 0.997). Conclusions: Diode laser can be used as a root canal disinfectant alone or in combination with chlorhexidine.
format Online
Article
Text
id pubmed-9521298
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Cureus
record_format MEDLINE/PubMed
spelling pubmed-95212982022-09-30 Comparative Evaluation of the Antibacterial Efficacy of Chlorhexidine and 810 nm Diode Laser in the Disinfection of Root Canals Contaminated With Enterococcus faecalis: An In Vitro Study Mathew, Teris BM, Shanthala GV, Prashanth Jose, Jubin Cureus Dentistry Introduction: This study compared the antibacterial efficacy of three different root canal irrigants, sodium hypochlorite, chlorhexidine and 810 nm diode laser and diode laser in combination with chlorhexidine in root canals contaminated with Enterococcus faecalis. Methods: Fifty extracted mandibular first premolars were decoronated at the cementoenamel junction and cut at the apical end; biomechanical preparation was done, autoclaved and contaminated with Enterococcus faecalis. The specimens were divided into five groups containing 10 teeth each: group I: saline (negative control), group II: 2.5% sodium hypochlorite, group III: 2% chlorhexidine gluconate solution, group IV: diode laser, and group V: diode laser in combination with 2% chlorhexidine. Disinfection was carried out, after which dentinal shavings were collected from the specimens of each group. Antimicrobial efficacy was tested by counting the colony-forming units of viable Enterococcus faecalis on the agar plates. One-way ANOVA and Scheffe’s post hoc test were done to analyse the results. Results: Diode laser with chlorhexidine group showed minimum colony-forming units followed by the diode laser group. The post hoc test showed a statistically significant difference between saline, 2.5% sodium hypochlorite and 2% chlorhexidine groups (P = 0.001) and a non-significant difference between diode laser and diode laser with chlorhexidine group (P = 0.997). Conclusions: Diode laser can be used as a root canal disinfectant alone or in combination with chlorhexidine. Cureus 2022-08-30 /pmc/articles/PMC9521298/ /pubmed/36185833 http://dx.doi.org/10.7759/cureus.28596 Text en Copyright © 2022, Mathew et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Dentistry
Mathew, Teris
BM, Shanthala
GV, Prashanth
Jose, Jubin
Comparative Evaluation of the Antibacterial Efficacy of Chlorhexidine and 810 nm Diode Laser in the Disinfection of Root Canals Contaminated With Enterococcus faecalis: An In Vitro Study
title Comparative Evaluation of the Antibacterial Efficacy of Chlorhexidine and 810 nm Diode Laser in the Disinfection of Root Canals Contaminated With Enterococcus faecalis: An In Vitro Study
title_full Comparative Evaluation of the Antibacterial Efficacy of Chlorhexidine and 810 nm Diode Laser in the Disinfection of Root Canals Contaminated With Enterococcus faecalis: An In Vitro Study
title_fullStr Comparative Evaluation of the Antibacterial Efficacy of Chlorhexidine and 810 nm Diode Laser in the Disinfection of Root Canals Contaminated With Enterococcus faecalis: An In Vitro Study
title_full_unstemmed Comparative Evaluation of the Antibacterial Efficacy of Chlorhexidine and 810 nm Diode Laser in the Disinfection of Root Canals Contaminated With Enterococcus faecalis: An In Vitro Study
title_short Comparative Evaluation of the Antibacterial Efficacy of Chlorhexidine and 810 nm Diode Laser in the Disinfection of Root Canals Contaminated With Enterococcus faecalis: An In Vitro Study
title_sort comparative evaluation of the antibacterial efficacy of chlorhexidine and 810 nm diode laser in the disinfection of root canals contaminated with enterococcus faecalis: an in vitro study
topic Dentistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9521298/
https://www.ncbi.nlm.nih.gov/pubmed/36185833
http://dx.doi.org/10.7759/cureus.28596
work_keys_str_mv AT mathewteris comparativeevaluationoftheantibacterialefficacyofchlorhexidineand810nmdiodelaserinthedisinfectionofrootcanalscontaminatedwithenterococcusfaecalisaninvitrostudy
AT bmshanthala comparativeevaluationoftheantibacterialefficacyofchlorhexidineand810nmdiodelaserinthedisinfectionofrootcanalscontaminatedwithenterococcusfaecalisaninvitrostudy
AT gvprashanth comparativeevaluationoftheantibacterialefficacyofchlorhexidineand810nmdiodelaserinthedisinfectionofrootcanalscontaminatedwithenterococcusfaecalisaninvitrostudy
AT josejubin comparativeevaluationoftheantibacterialefficacyofchlorhexidineand810nmdiodelaserinthedisinfectionofrootcanalscontaminatedwithenterococcusfaecalisaninvitrostudy