Hybrid cell line development system utilizing site-specific integration and methotrexate-mediated gene amplification in Chinese hamster ovary cells

Site-specific integration has emerged as a promising strategy for streamlined and predictable Chinese hamster ovary (CHO) cell line development (CLD). However, the low specific productivity of the targeted integrants limits their practical application. In this study, we developed a hybrid CLD platfo...

Descripción completa

Detalles Bibliográficos
Autores principales: Min, Honggi, Kim, Seul Mi, Kim, Dongwoo, Lee, Solhwi, Lee, Sumin, Lee, Jae Seong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9521551/
https://www.ncbi.nlm.nih.gov/pubmed/36185448
http://dx.doi.org/10.3389/fbioe.2022.977193

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9521551/
https://www.ncbi.nlm.nih.gov/pubmed/36185448
http://dx.doi.org/10.3389/fbioe.2022.977193

Ejemplares similares