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Second harmonic generation nanoparticles enables Near-Infrared Photodynamic Therapy from visible light reactive photosensitizer conjugates

Combination of photosensitizers (PS) with nanotechnology can improve the therapeutic efficiency of clinical Photodynamic Therapy (PDT) by converting visible light reactive PSs into Near-Infrared (NIR) light responsive molecules using Harmonic Nanoparticles (HNP). To test the PDT efficiency of HNP-PS...

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Autores principales: Barbora, Ayan, Yazbak, Fares, Lyssenko, Svetlana, Nave, Vadim, Nakonechny, Faina, Ben Ishai, Paul, Minnes, Refael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9522301/
https://www.ncbi.nlm.nih.gov/pubmed/36173987
http://dx.doi.org/10.1371/journal.pone.0274954
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author Barbora, Ayan
Yazbak, Fares
Lyssenko, Svetlana
Nave, Vadim
Nakonechny, Faina
Ben Ishai, Paul
Minnes, Refael
author_facet Barbora, Ayan
Yazbak, Fares
Lyssenko, Svetlana
Nave, Vadim
Nakonechny, Faina
Ben Ishai, Paul
Minnes, Refael
author_sort Barbora, Ayan
collection PubMed
description Combination of photosensitizers (PS) with nanotechnology can improve the therapeutic efficiency of clinical Photodynamic Therapy (PDT) by converting visible light reactive PSs into Near-Infrared (NIR) light responsive molecules using Harmonic Nanoparticles (HNP). To test the PDT efficiency of HNP-PS conjugates, pathogenic S. aureus cell cultures were treated with perovskite (Barium Titanate) Second Harmonic Generation (SHG) nanoparticles conjugated to photosensitizers (PS) (we compared both FDA approved Protoporphyrin IX and Curcumin) and subjected to a femtosecond pulsed Near-Infrared (NIR) laser (800 nm, 232–228 mW, 12–15 fs pulse width at repetition rate of 76.9 MHz) for 10 minutes each. NIR PDT using Barium Titanate (BT) conjugated with Protoporphyrin IX as HNP-PS conjugate reduced the viability of S. aureus cells by 77.3 ± 9.7% while BT conjugated with Curcumin did not elicit any significant effect. Conventional PSs reactive only to visible spectrum light coupled with SHG nanoparticles enables the use of higher tissue penetrating NIR light to generate an efficient photodynamic effect, thereby overcoming low light penetration and tissue specificity of conventional visible light PDT treatments.
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spelling pubmed-95223012022-09-30 Second harmonic generation nanoparticles enables Near-Infrared Photodynamic Therapy from visible light reactive photosensitizer conjugates Barbora, Ayan Yazbak, Fares Lyssenko, Svetlana Nave, Vadim Nakonechny, Faina Ben Ishai, Paul Minnes, Refael PLoS One Research Article Combination of photosensitizers (PS) with nanotechnology can improve the therapeutic efficiency of clinical Photodynamic Therapy (PDT) by converting visible light reactive PSs into Near-Infrared (NIR) light responsive molecules using Harmonic Nanoparticles (HNP). To test the PDT efficiency of HNP-PS conjugates, pathogenic S. aureus cell cultures were treated with perovskite (Barium Titanate) Second Harmonic Generation (SHG) nanoparticles conjugated to photosensitizers (PS) (we compared both FDA approved Protoporphyrin IX and Curcumin) and subjected to a femtosecond pulsed Near-Infrared (NIR) laser (800 nm, 232–228 mW, 12–15 fs pulse width at repetition rate of 76.9 MHz) for 10 minutes each. NIR PDT using Barium Titanate (BT) conjugated with Protoporphyrin IX as HNP-PS conjugate reduced the viability of S. aureus cells by 77.3 ± 9.7% while BT conjugated with Curcumin did not elicit any significant effect. Conventional PSs reactive only to visible spectrum light coupled with SHG nanoparticles enables the use of higher tissue penetrating NIR light to generate an efficient photodynamic effect, thereby overcoming low light penetration and tissue specificity of conventional visible light PDT treatments. Public Library of Science 2022-09-29 /pmc/articles/PMC9522301/ /pubmed/36173987 http://dx.doi.org/10.1371/journal.pone.0274954 Text en © 2022 Barbora et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Barbora, Ayan
Yazbak, Fares
Lyssenko, Svetlana
Nave, Vadim
Nakonechny, Faina
Ben Ishai, Paul
Minnes, Refael
Second harmonic generation nanoparticles enables Near-Infrared Photodynamic Therapy from visible light reactive photosensitizer conjugates
title Second harmonic generation nanoparticles enables Near-Infrared Photodynamic Therapy from visible light reactive photosensitizer conjugates
title_full Second harmonic generation nanoparticles enables Near-Infrared Photodynamic Therapy from visible light reactive photosensitizer conjugates
title_fullStr Second harmonic generation nanoparticles enables Near-Infrared Photodynamic Therapy from visible light reactive photosensitizer conjugates
title_full_unstemmed Second harmonic generation nanoparticles enables Near-Infrared Photodynamic Therapy from visible light reactive photosensitizer conjugates
title_short Second harmonic generation nanoparticles enables Near-Infrared Photodynamic Therapy from visible light reactive photosensitizer conjugates
title_sort second harmonic generation nanoparticles enables near-infrared photodynamic therapy from visible light reactive photosensitizer conjugates
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9522301/
https://www.ncbi.nlm.nih.gov/pubmed/36173987
http://dx.doi.org/10.1371/journal.pone.0274954
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