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Amelioration of aluminum-induced hepatic and nephrotoxicity by Premna odorata extract is mediated by lowering MMP9 and TGF-β gene alterations in Wistar rat
This study aims to investigate the effect of Premna odorata (P. odorata) (Lamiaceae) on the hepatic and nephrotoxicity induced by aluminum chloride (AlCl(3)) in rat. Wistar male rats were equally classified into four groups: control, P. odorata extract (500 mg/kg B.W.), AlCl(3) (70 mg/kg B.W.), and...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Berlin Heidelberg
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9522688/ https://www.ncbi.nlm.nih.gov/pubmed/35614356 http://dx.doi.org/10.1007/s11356-022-20735-8 |
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author | Ahmed, Walaa M. S. Ibrahim, Marwa A. Helmy, Nermeen A. ElKashlan, Akram M. Elmaidomy, Abeer H. Zaki, Amr R. |
author_facet | Ahmed, Walaa M. S. Ibrahim, Marwa A. Helmy, Nermeen A. ElKashlan, Akram M. Elmaidomy, Abeer H. Zaki, Amr R. |
author_sort | Ahmed, Walaa M. S. |
collection | PubMed |
description | This study aims to investigate the effect of Premna odorata (P. odorata) (Lamiaceae) on the hepatic and nephrotoxicity induced by aluminum chloride (AlCl(3)) in rat. Wistar male rats were equally classified into four groups: control, P. odorata extract (500 mg/kg B.W.), AlCl(3) (70 mg/kg B.W.), and P. odorata extract plus AlCl(3) groups. All treatments were given orally for 4 weeks. Serum transaminases and some biochemical parameters, hepatic and renal antioxidant/oxidant biomarker; tumor necrosis factor-α (TNF-α); matrix metalloproteinase (MMP9) and transforming growth factor-β (TGF-β) mRNA expression; histopathological examination of the liver, and kidneys were investigated. The obtained results revealed that AlCl(3) significantly increased the activities of serum aspartate transaminase, alanine transaminase, and alkaline phosphatase as well as produced a significant increase in total cholesterol, triglyceride, urea, and creatinine concentrations, while there were no changes observed in the total protein, albumin, and globulin concentrations. Also, aluminum administration significantly decreased the reduced glutathione content and increased the catalase activity, malondialdehyde, and TNF-α concentrations in the liver and kidney tissue. Moreover, AlCl(3) results in congestion, degeneration, and inflammation of the liver and kidney tissue. Co-treatment of P. odorata extract with AlCl(3) alleviated its harmful effects on the previous parameters and reduced the histopathological alterations induced by AlCl(3). Therefore, Premna odorata may have a potent protective effect against oxidative stress induced by Al toxicity through downregulation of MMP9 and TGF-β gene expression. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11356-022-20735-8. |
format | Online Article Text |
id | pubmed-9522688 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-95226882022-10-01 Amelioration of aluminum-induced hepatic and nephrotoxicity by Premna odorata extract is mediated by lowering MMP9 and TGF-β gene alterations in Wistar rat Ahmed, Walaa M. S. Ibrahim, Marwa A. Helmy, Nermeen A. ElKashlan, Akram M. Elmaidomy, Abeer H. Zaki, Amr R. Environ Sci Pollut Res Int Research Article This study aims to investigate the effect of Premna odorata (P. odorata) (Lamiaceae) on the hepatic and nephrotoxicity induced by aluminum chloride (AlCl(3)) in rat. Wistar male rats were equally classified into four groups: control, P. odorata extract (500 mg/kg B.W.), AlCl(3) (70 mg/kg B.W.), and P. odorata extract plus AlCl(3) groups. All treatments were given orally for 4 weeks. Serum transaminases and some biochemical parameters, hepatic and renal antioxidant/oxidant biomarker; tumor necrosis factor-α (TNF-α); matrix metalloproteinase (MMP9) and transforming growth factor-β (TGF-β) mRNA expression; histopathological examination of the liver, and kidneys were investigated. The obtained results revealed that AlCl(3) significantly increased the activities of serum aspartate transaminase, alanine transaminase, and alkaline phosphatase as well as produced a significant increase in total cholesterol, triglyceride, urea, and creatinine concentrations, while there were no changes observed in the total protein, albumin, and globulin concentrations. Also, aluminum administration significantly decreased the reduced glutathione content and increased the catalase activity, malondialdehyde, and TNF-α concentrations in the liver and kidney tissue. Moreover, AlCl(3) results in congestion, degeneration, and inflammation of the liver and kidney tissue. Co-treatment of P. odorata extract with AlCl(3) alleviated its harmful effects on the previous parameters and reduced the histopathological alterations induced by AlCl(3). Therefore, Premna odorata may have a potent protective effect against oxidative stress induced by Al toxicity through downregulation of MMP9 and TGF-β gene expression. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11356-022-20735-8. Springer Berlin Heidelberg 2022-05-26 2022 /pmc/articles/PMC9522688/ /pubmed/35614356 http://dx.doi.org/10.1007/s11356-022-20735-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Ahmed, Walaa M. S. Ibrahim, Marwa A. Helmy, Nermeen A. ElKashlan, Akram M. Elmaidomy, Abeer H. Zaki, Amr R. Amelioration of aluminum-induced hepatic and nephrotoxicity by Premna odorata extract is mediated by lowering MMP9 and TGF-β gene alterations in Wistar rat |
title | Amelioration of aluminum-induced hepatic and nephrotoxicity by Premna odorata extract is mediated by lowering MMP9 and TGF-β gene alterations in Wistar rat |
title_full | Amelioration of aluminum-induced hepatic and nephrotoxicity by Premna odorata extract is mediated by lowering MMP9 and TGF-β gene alterations in Wistar rat |
title_fullStr | Amelioration of aluminum-induced hepatic and nephrotoxicity by Premna odorata extract is mediated by lowering MMP9 and TGF-β gene alterations in Wistar rat |
title_full_unstemmed | Amelioration of aluminum-induced hepatic and nephrotoxicity by Premna odorata extract is mediated by lowering MMP9 and TGF-β gene alterations in Wistar rat |
title_short | Amelioration of aluminum-induced hepatic and nephrotoxicity by Premna odorata extract is mediated by lowering MMP9 and TGF-β gene alterations in Wistar rat |
title_sort | amelioration of aluminum-induced hepatic and nephrotoxicity by premna odorata extract is mediated by lowering mmp9 and tgf-β gene alterations in wistar rat |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9522688/ https://www.ncbi.nlm.nih.gov/pubmed/35614356 http://dx.doi.org/10.1007/s11356-022-20735-8 |
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