Cargando…

Convergence of biofilm successional trajectories initiated during contrasting seasons

Biofilm communities play a major role in explaining the temporal variation of biogeochemical conditions in freshwater ecosystems, and yet we know little about how these complex microbial communities change over time (aka succession), and from different initial conditions, in comparison to other stre...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Jing, Peipoch, Marc, Guo, Xiaoxiao, Kan, Jinjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9522907/
https://www.ncbi.nlm.nih.gov/pubmed/36187986
http://dx.doi.org/10.3389/fmicb.2022.991816
_version_ 1784800158320427008
author Wang, Jing
Peipoch, Marc
Guo, Xiaoxiao
Kan, Jinjun
author_facet Wang, Jing
Peipoch, Marc
Guo, Xiaoxiao
Kan, Jinjun
author_sort Wang, Jing
collection PubMed
description Biofilm communities play a major role in explaining the temporal variation of biogeochemical conditions in freshwater ecosystems, and yet we know little about how these complex microbial communities change over time (aka succession), and from different initial conditions, in comparison to other stream communities. This has resulted in limited knowledge on how biofilm community structure and microbial colonization vary over relevant time scales to become mature biofilms capable of significant alteration of the freshwater environment in which they live. Here, we monitored successional trajectories of biofilm communities from summer and winter in a headwater stream and evaluated their structural state over time by DNA high-throughput sequencing. Significant differences in biofilm composition were observed when microbial colonization started in the summer vs. winter seasons, with higher percentage of algae (Bacillariophyta) and Bacteroidetes in winter-initiated samples but higher abundance of Proteobacteria (e.g., Rhizobiales, Rhodobacterales, Sphingomonadales, and Burkholderiales), Actinobacteria, and Chloroflexi in summer-initiated samples. Interestingly, results showed that despite seasonal effects on early biofilm succession, biofilm community structures converged after 70 days, suggesting the existence of a stable, mature community in the stream that is independent of the environmental conditions during biofilm colonization. Overall, our results show that algae are important in the early development of biofilm communities during winter, while heterotrophic bacteria play a more critical role during summer colonization and development of biofilms.
format Online
Article
Text
id pubmed-9522907
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-95229072022-10-01 Convergence of biofilm successional trajectories initiated during contrasting seasons Wang, Jing Peipoch, Marc Guo, Xiaoxiao Kan, Jinjun Front Microbiol Microbiology Biofilm communities play a major role in explaining the temporal variation of biogeochemical conditions in freshwater ecosystems, and yet we know little about how these complex microbial communities change over time (aka succession), and from different initial conditions, in comparison to other stream communities. This has resulted in limited knowledge on how biofilm community structure and microbial colonization vary over relevant time scales to become mature biofilms capable of significant alteration of the freshwater environment in which they live. Here, we monitored successional trajectories of biofilm communities from summer and winter in a headwater stream and evaluated their structural state over time by DNA high-throughput sequencing. Significant differences in biofilm composition were observed when microbial colonization started in the summer vs. winter seasons, with higher percentage of algae (Bacillariophyta) and Bacteroidetes in winter-initiated samples but higher abundance of Proteobacteria (e.g., Rhizobiales, Rhodobacterales, Sphingomonadales, and Burkholderiales), Actinobacteria, and Chloroflexi in summer-initiated samples. Interestingly, results showed that despite seasonal effects on early biofilm succession, biofilm community structures converged after 70 days, suggesting the existence of a stable, mature community in the stream that is independent of the environmental conditions during biofilm colonization. Overall, our results show that algae are important in the early development of biofilm communities during winter, while heterotrophic bacteria play a more critical role during summer colonization and development of biofilms. Frontiers Media S.A. 2022-09-16 /pmc/articles/PMC9522907/ /pubmed/36187986 http://dx.doi.org/10.3389/fmicb.2022.991816 Text en Copyright © 2022 Wang, Peipoch, Guo and Kan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wang, Jing
Peipoch, Marc
Guo, Xiaoxiao
Kan, Jinjun
Convergence of biofilm successional trajectories initiated during contrasting seasons
title Convergence of biofilm successional trajectories initiated during contrasting seasons
title_full Convergence of biofilm successional trajectories initiated during contrasting seasons
title_fullStr Convergence of biofilm successional trajectories initiated during contrasting seasons
title_full_unstemmed Convergence of biofilm successional trajectories initiated during contrasting seasons
title_short Convergence of biofilm successional trajectories initiated during contrasting seasons
title_sort convergence of biofilm successional trajectories initiated during contrasting seasons
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9522907/
https://www.ncbi.nlm.nih.gov/pubmed/36187986
http://dx.doi.org/10.3389/fmicb.2022.991816
work_keys_str_mv AT wangjing convergenceofbiofilmsuccessionaltrajectoriesinitiatedduringcontrastingseasons
AT peipochmarc convergenceofbiofilmsuccessionaltrajectoriesinitiatedduringcontrastingseasons
AT guoxiaoxiao convergenceofbiofilmsuccessionaltrajectoriesinitiatedduringcontrastingseasons
AT kanjinjun convergenceofbiofilmsuccessionaltrajectoriesinitiatedduringcontrastingseasons