Cargando…

In vitro investigation on lactic acid bacteria isolatedfrom Yak faeces for potential probiotics

In order to evaluate the potential and safety of lactic acid bacteria (LAB) isolated from faeces samples of Ganan yak as probiotic for prevention and/or treatment of yak diarrhea, four strains of LAB including Latilactobacillus curvatus (FY1), Weissella cibaria (FY2), Limosilactobacillus mucosae (FY...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Qingli, Wang, Meng, Ma, Xin, Li, Zhijie, Jiang, Chenghui, Pan, Yangyang, Zeng, Qiaoying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9523120/
https://www.ncbi.nlm.nih.gov/pubmed/36189367
http://dx.doi.org/10.3389/fcimb.2022.984537
_version_ 1784800201412706304
author Zhang, Qingli
Wang, Meng
Ma, Xin
Li, Zhijie
Jiang, Chenghui
Pan, Yangyang
Zeng, Qiaoying
author_facet Zhang, Qingli
Wang, Meng
Ma, Xin
Li, Zhijie
Jiang, Chenghui
Pan, Yangyang
Zeng, Qiaoying
author_sort Zhang, Qingli
collection PubMed
description In order to evaluate the potential and safety of lactic acid bacteria (LAB) isolated from faeces samples of Ganan yak as probiotic for prevention and/or treatment of yak diarrhea, four strains of LAB including Latilactobacillus curvatus (FY1), Weissella cibaria (FY2), Limosilactobacillus mucosae (FY3), and Lactiplantibacillus pentosus (FY4) were isolated and identified in this study. Cell surface characteristics (hydrophobicity and cell aggregation), acid resistance and bile tolerance, compatibility, antibacterial activity and in vitro cell adhesion tests were also carried out to evaluate the probiotic potential of LAB. The results showed that the four isolates had certain acid tolerance, bile salt tolerance, hydrophobicity and cell aggregation, all of which contribute to the survival and colonization of LAB in the gastrointestinal tract. There is no compatibility between the four strains, so they can be combined into a mixed probiotic formula. Antimicrobial tests showed that the four strains were antagonistic to Escherichia coli, Staphylococcus aureus, and Salmonella typhimurium. Moreover, the in vitro safety of the four isolates were determined through hemolytic analysis, gelatinase activity, and antibacterial susceptibility experiments. The results suggest that all the four strains were considered as safe because they had no hemolytic activity, no gelatinase activity and were sensitive to most antibacterial agents. Moreover, the acute oral toxicity test of LAB had no adverse effect on body weight gain, food utilization and organ indices in Kunming mice. In conclusion, the four LAB isolated from yak feces have considerable potential to prevent and/or treat yak bacterial disease-related diarrhea.
format Online
Article
Text
id pubmed-9523120
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-95231202022-10-01 In vitro investigation on lactic acid bacteria isolatedfrom Yak faeces for potential probiotics Zhang, Qingli Wang, Meng Ma, Xin Li, Zhijie Jiang, Chenghui Pan, Yangyang Zeng, Qiaoying Front Cell Infect Microbiol Cellular and Infection Microbiology In order to evaluate the potential and safety of lactic acid bacteria (LAB) isolated from faeces samples of Ganan yak as probiotic for prevention and/or treatment of yak diarrhea, four strains of LAB including Latilactobacillus curvatus (FY1), Weissella cibaria (FY2), Limosilactobacillus mucosae (FY3), and Lactiplantibacillus pentosus (FY4) were isolated and identified in this study. Cell surface characteristics (hydrophobicity and cell aggregation), acid resistance and bile tolerance, compatibility, antibacterial activity and in vitro cell adhesion tests were also carried out to evaluate the probiotic potential of LAB. The results showed that the four isolates had certain acid tolerance, bile salt tolerance, hydrophobicity and cell aggregation, all of which contribute to the survival and colonization of LAB in the gastrointestinal tract. There is no compatibility between the four strains, so they can be combined into a mixed probiotic formula. Antimicrobial tests showed that the four strains were antagonistic to Escherichia coli, Staphylococcus aureus, and Salmonella typhimurium. Moreover, the in vitro safety of the four isolates were determined through hemolytic analysis, gelatinase activity, and antibacterial susceptibility experiments. The results suggest that all the four strains were considered as safe because they had no hemolytic activity, no gelatinase activity and were sensitive to most antibacterial agents. Moreover, the acute oral toxicity test of LAB had no adverse effect on body weight gain, food utilization and organ indices in Kunming mice. In conclusion, the four LAB isolated from yak feces have considerable potential to prevent and/or treat yak bacterial disease-related diarrhea. Frontiers Media S.A. 2022-09-16 /pmc/articles/PMC9523120/ /pubmed/36189367 http://dx.doi.org/10.3389/fcimb.2022.984537 Text en Copyright © 2022 Zhang, Wang, Ma, Li, Jiang, Pan and Zeng https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Zhang, Qingli
Wang, Meng
Ma, Xin
Li, Zhijie
Jiang, Chenghui
Pan, Yangyang
Zeng, Qiaoying
In vitro investigation on lactic acid bacteria isolatedfrom Yak faeces for potential probiotics
title In vitro investigation on lactic acid bacteria isolatedfrom Yak faeces for potential probiotics
title_full In vitro investigation on lactic acid bacteria isolatedfrom Yak faeces for potential probiotics
title_fullStr In vitro investigation on lactic acid bacteria isolatedfrom Yak faeces for potential probiotics
title_full_unstemmed In vitro investigation on lactic acid bacteria isolatedfrom Yak faeces for potential probiotics
title_short In vitro investigation on lactic acid bacteria isolatedfrom Yak faeces for potential probiotics
title_sort in vitro investigation on lactic acid bacteria isolatedfrom yak faeces for potential probiotics
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9523120/
https://www.ncbi.nlm.nih.gov/pubmed/36189367
http://dx.doi.org/10.3389/fcimb.2022.984537
work_keys_str_mv AT zhangqingli invitroinvestigationonlacticacidbacteriaisolatedfromyakfaecesforpotentialprobiotics
AT wangmeng invitroinvestigationonlacticacidbacteriaisolatedfromyakfaecesforpotentialprobiotics
AT maxin invitroinvestigationonlacticacidbacteriaisolatedfromyakfaecesforpotentialprobiotics
AT lizhijie invitroinvestigationonlacticacidbacteriaisolatedfromyakfaecesforpotentialprobiotics
AT jiangchenghui invitroinvestigationonlacticacidbacteriaisolatedfromyakfaecesforpotentialprobiotics
AT panyangyang invitroinvestigationonlacticacidbacteriaisolatedfromyakfaecesforpotentialprobiotics
AT zengqiaoying invitroinvestigationonlacticacidbacteriaisolatedfromyakfaecesforpotentialprobiotics