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LncRNA affects epigenetic reprogramming of porcine embryo development by regulating global epigenetic modification and the downstream gene SIN3A

The study of preimplantation development is of great significance to reproductive biology and regenerative medicine. With the development of high-throughput deep sequencing technology, it has been found that lncRNAs play a very important role in the regulation of embryonic development. In this study...

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Autores principales: Zhang, Daoyu, Zhou, Yongfeng, Huang, Rong, Zhai, Yanhui, Wu, Di, An, Xinglan, Zhang, Sheng, Shi, Lijing, Li, Qi, Kong, Xiangjie, Yu, Hao, Li, Ziyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9523245/
https://www.ncbi.nlm.nih.gov/pubmed/36187791
http://dx.doi.org/10.3389/fphys.2022.971965
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author Zhang, Daoyu
Zhou, Yongfeng
Huang, Rong
Zhai, Yanhui
Wu, Di
An, Xinglan
Zhang, Sheng
Shi, Lijing
Li, Qi
Kong, Xiangjie
Yu, Hao
Li, Ziyi
author_facet Zhang, Daoyu
Zhou, Yongfeng
Huang, Rong
Zhai, Yanhui
Wu, Di
An, Xinglan
Zhang, Sheng
Shi, Lijing
Li, Qi
Kong, Xiangjie
Yu, Hao
Li, Ziyi
author_sort Zhang, Daoyu
collection PubMed
description The study of preimplantation development is of great significance to reproductive biology and regenerative medicine. With the development of high-throughput deep sequencing technology, it has been found that lncRNAs play a very important role in the regulation of embryonic development. In this study, key lncRNAs that regulate embryonic development were screened by analyzing the expression pattern of lncRNAs in porcine in vivo fertilization (IVV) embryos. By knocking down lncRNA expression in in vitro fertilization (IVF) embryos, we investigated its function and mechanism of regulating embryonic development. The results showed that the expression pattern of lncRNA was consistent with the time of gene activation. The lncRNAs were highly expressed in the 4-cell to blastocyst stage but barely expressed in the oocytes and 2-cell stage. So we speculated this part of lncRNAs may regulate gene expression. The lncRNA LOC102165808 (named lncT because the gene near this lncRNA is TFAP2C) was one of them. The knockdown (KD) of lncT inhibited embryonic development, resulting in decreased H3K4me3, H3K4me2, and H3K9me3, and increased DNA methylation. Meanwhile, RNAseq showed SIN3A was the top decreased gene in lncT-KD embryos. There was a severe blastocyst formation defect in SIN3A-KD embryos. Both lncT and SIN3A could affect NANOG and induce more cell apoptosis. In conclusion, the knockdown of lncT inhibits embryonic development by regulating H3K4me3, H3K4me2, DNA methylation, pluripotency gene, and apoptosis, and SIN3A is one of the downstream genes of lncT in regulating embryonic development.
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spelling pubmed-95232452022-10-01 LncRNA affects epigenetic reprogramming of porcine embryo development by regulating global epigenetic modification and the downstream gene SIN3A Zhang, Daoyu Zhou, Yongfeng Huang, Rong Zhai, Yanhui Wu, Di An, Xinglan Zhang, Sheng Shi, Lijing Li, Qi Kong, Xiangjie Yu, Hao Li, Ziyi Front Physiol Physiology The study of preimplantation development is of great significance to reproductive biology and regenerative medicine. With the development of high-throughput deep sequencing technology, it has been found that lncRNAs play a very important role in the regulation of embryonic development. In this study, key lncRNAs that regulate embryonic development were screened by analyzing the expression pattern of lncRNAs in porcine in vivo fertilization (IVV) embryos. By knocking down lncRNA expression in in vitro fertilization (IVF) embryos, we investigated its function and mechanism of regulating embryonic development. The results showed that the expression pattern of lncRNA was consistent with the time of gene activation. The lncRNAs were highly expressed in the 4-cell to blastocyst stage but barely expressed in the oocytes and 2-cell stage. So we speculated this part of lncRNAs may regulate gene expression. The lncRNA LOC102165808 (named lncT because the gene near this lncRNA is TFAP2C) was one of them. The knockdown (KD) of lncT inhibited embryonic development, resulting in decreased H3K4me3, H3K4me2, and H3K9me3, and increased DNA methylation. Meanwhile, RNAseq showed SIN3A was the top decreased gene in lncT-KD embryos. There was a severe blastocyst formation defect in SIN3A-KD embryos. Both lncT and SIN3A could affect NANOG and induce more cell apoptosis. In conclusion, the knockdown of lncT inhibits embryonic development by regulating H3K4me3, H3K4me2, DNA methylation, pluripotency gene, and apoptosis, and SIN3A is one of the downstream genes of lncT in regulating embryonic development. Frontiers Media S.A. 2022-09-16 /pmc/articles/PMC9523245/ /pubmed/36187791 http://dx.doi.org/10.3389/fphys.2022.971965 Text en Copyright © 2022 Zhang, Zhou, Huang, Zhai, Wu, An, Zhang, Shi, Li, Kong, Yu and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Zhang, Daoyu
Zhou, Yongfeng
Huang, Rong
Zhai, Yanhui
Wu, Di
An, Xinglan
Zhang, Sheng
Shi, Lijing
Li, Qi
Kong, Xiangjie
Yu, Hao
Li, Ziyi
LncRNA affects epigenetic reprogramming of porcine embryo development by regulating global epigenetic modification and the downstream gene SIN3A
title LncRNA affects epigenetic reprogramming of porcine embryo development by regulating global epigenetic modification and the downstream gene SIN3A
title_full LncRNA affects epigenetic reprogramming of porcine embryo development by regulating global epigenetic modification and the downstream gene SIN3A
title_fullStr LncRNA affects epigenetic reprogramming of porcine embryo development by regulating global epigenetic modification and the downstream gene SIN3A
title_full_unstemmed LncRNA affects epigenetic reprogramming of porcine embryo development by regulating global epigenetic modification and the downstream gene SIN3A
title_short LncRNA affects epigenetic reprogramming of porcine embryo development by regulating global epigenetic modification and the downstream gene SIN3A
title_sort lncrna affects epigenetic reprogramming of porcine embryo development by regulating global epigenetic modification and the downstream gene sin3a
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9523245/
https://www.ncbi.nlm.nih.gov/pubmed/36187791
http://dx.doi.org/10.3389/fphys.2022.971965
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