Cargando…

Molecular characterization of chicken anemia virus in Guangxi Province, southern China, from 2018 to 2020

BACKGROUND: Chicken anemia virus (CAV) causes chicken infectious anemia, which results in immunosuppression; the virus has spread widely in chicken flocks in China. OBJECTIVES: The aim of this study was to understand recent CAV genetic evolution in chicken flocks in Guangxi Province, southern China....

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Minxiu, Deng, Xianwen, Xie, Zhixun, Zhang, Yanfang, Xie, Zhiqin, Xie, Liji, Luo, Sisi, Fan, Qing, Zeng, Tingting, Huang, Jiaoling, Wang, Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9523344/
https://www.ncbi.nlm.nih.gov/pubmed/36038184
http://dx.doi.org/10.4142/jvs.22023
_version_ 1784800267794907136
author Zhang, Minxiu
Deng, Xianwen
Xie, Zhixun
Zhang, Yanfang
Xie, Zhiqin
Xie, Liji
Luo, Sisi
Fan, Qing
Zeng, Tingting
Huang, Jiaoling
Wang, Sheng
author_facet Zhang, Minxiu
Deng, Xianwen
Xie, Zhixun
Zhang, Yanfang
Xie, Zhiqin
Xie, Liji
Luo, Sisi
Fan, Qing
Zeng, Tingting
Huang, Jiaoling
Wang, Sheng
author_sort Zhang, Minxiu
collection PubMed
description BACKGROUND: Chicken anemia virus (CAV) causes chicken infectious anemia, which results in immunosuppression; the virus has spread widely in chicken flocks in China. OBJECTIVES: The aim of this study was to understand recent CAV genetic evolution in chicken flocks in Guangxi Province, southern China. METHODS: In total, 350 liver samples were collected from eight commercial broiler chicken farms in Guangxi Province in southern China from 2018 to 2020. CAV was detected by conventional PCR, and twenty CAV complete genomes were amplified and used for the phylogenetic analysis and recombination analysis. RESULTS: The overall CAV-positive rate was 17.1%. The genetic analysis revealed that 84 CAVs were distributed in groups A, B, C (subgroups C1-C3) and D. In total, 30 of 47 Chinese CAV sequences from 2005-2020 belong to subgroup C3, including 15 CAVs from this study. There were some specific mutation sites among the intergenotypes in the VP1 protein. The amino acids at position 394Q in the VP1 protein of 20 CAV strains were consistent with the characteristics of a highly pathogenic strain. GX1904B was a putative recombinant. CONCLUSIONS: Subgroup C3 was the dominant genotype in Guangxi Province from 2018–2020. The 20 CAV strains in this study might be virulent according to the amino acid residue analysis. These data help improve our understanding of the epidemiological trends of CAV in southern China.
format Online
Article
Text
id pubmed-9523344
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher The Korean Society of Veterinary Science
record_format MEDLINE/PubMed
spelling pubmed-95233442022-10-11 Molecular characterization of chicken anemia virus in Guangxi Province, southern China, from 2018 to 2020 Zhang, Minxiu Deng, Xianwen Xie, Zhixun Zhang, Yanfang Xie, Zhiqin Xie, Liji Luo, Sisi Fan, Qing Zeng, Tingting Huang, Jiaoling Wang, Sheng J Vet Sci Original Article BACKGROUND: Chicken anemia virus (CAV) causes chicken infectious anemia, which results in immunosuppression; the virus has spread widely in chicken flocks in China. OBJECTIVES: The aim of this study was to understand recent CAV genetic evolution in chicken flocks in Guangxi Province, southern China. METHODS: In total, 350 liver samples were collected from eight commercial broiler chicken farms in Guangxi Province in southern China from 2018 to 2020. CAV was detected by conventional PCR, and twenty CAV complete genomes were amplified and used for the phylogenetic analysis and recombination analysis. RESULTS: The overall CAV-positive rate was 17.1%. The genetic analysis revealed that 84 CAVs were distributed in groups A, B, C (subgroups C1-C3) and D. In total, 30 of 47 Chinese CAV sequences from 2005-2020 belong to subgroup C3, including 15 CAVs from this study. There were some specific mutation sites among the intergenotypes in the VP1 protein. The amino acids at position 394Q in the VP1 protein of 20 CAV strains were consistent with the characteristics of a highly pathogenic strain. GX1904B was a putative recombinant. CONCLUSIONS: Subgroup C3 was the dominant genotype in Guangxi Province from 2018–2020. The 20 CAV strains in this study might be virulent according to the amino acid residue analysis. These data help improve our understanding of the epidemiological trends of CAV in southern China. The Korean Society of Veterinary Science 2022-07-08 /pmc/articles/PMC9523344/ /pubmed/36038184 http://dx.doi.org/10.4142/jvs.22023 Text en © 2022 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Zhang, Minxiu
Deng, Xianwen
Xie, Zhixun
Zhang, Yanfang
Xie, Zhiqin
Xie, Liji
Luo, Sisi
Fan, Qing
Zeng, Tingting
Huang, Jiaoling
Wang, Sheng
Molecular characterization of chicken anemia virus in Guangxi Province, southern China, from 2018 to 2020
title Molecular characterization of chicken anemia virus in Guangxi Province, southern China, from 2018 to 2020
title_full Molecular characterization of chicken anemia virus in Guangxi Province, southern China, from 2018 to 2020
title_fullStr Molecular characterization of chicken anemia virus in Guangxi Province, southern China, from 2018 to 2020
title_full_unstemmed Molecular characterization of chicken anemia virus in Guangxi Province, southern China, from 2018 to 2020
title_short Molecular characterization of chicken anemia virus in Guangxi Province, southern China, from 2018 to 2020
title_sort molecular characterization of chicken anemia virus in guangxi province, southern china, from 2018 to 2020
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9523344/
https://www.ncbi.nlm.nih.gov/pubmed/36038184
http://dx.doi.org/10.4142/jvs.22023
work_keys_str_mv AT zhangminxiu molecularcharacterizationofchickenanemiavirusinguangxiprovincesouthernchinafrom2018to2020
AT dengxianwen molecularcharacterizationofchickenanemiavirusinguangxiprovincesouthernchinafrom2018to2020
AT xiezhixun molecularcharacterizationofchickenanemiavirusinguangxiprovincesouthernchinafrom2018to2020
AT zhangyanfang molecularcharacterizationofchickenanemiavirusinguangxiprovincesouthernchinafrom2018to2020
AT xiezhiqin molecularcharacterizationofchickenanemiavirusinguangxiprovincesouthernchinafrom2018to2020
AT xieliji molecularcharacterizationofchickenanemiavirusinguangxiprovincesouthernchinafrom2018to2020
AT luosisi molecularcharacterizationofchickenanemiavirusinguangxiprovincesouthernchinafrom2018to2020
AT fanqing molecularcharacterizationofchickenanemiavirusinguangxiprovincesouthernchinafrom2018to2020
AT zengtingting molecularcharacterizationofchickenanemiavirusinguangxiprovincesouthernchinafrom2018to2020
AT huangjiaoling molecularcharacterizationofchickenanemiavirusinguangxiprovincesouthernchinafrom2018to2020
AT wangsheng molecularcharacterizationofchickenanemiavirusinguangxiprovincesouthernchinafrom2018to2020