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Carbon isotope and soluble metabolites reflect physiological status among contrasting faba bean genotypes in response to water deficit

Identification and validation of biomarkers and bioindicators to select genotypes with superior tolerance to water deficit (WD) under field conditions are paramount to plant breeding programs. However, the co-occurrence of different abiotic stresses such as WD, heat, and radiation makes it difficult...

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Autores principales: Muktadir, Md Abdul, Merchant, Andrew, Sadeque, Abdus, Tanveer, Mohsin, Adhikari, Kedar Nath, Huang, Liping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9523585/
https://www.ncbi.nlm.nih.gov/pubmed/36186012
http://dx.doi.org/10.3389/fpls.2022.955406
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author Muktadir, Md Abdul
Merchant, Andrew
Sadeque, Abdus
Tanveer, Mohsin
Adhikari, Kedar Nath
Huang, Liping
author_facet Muktadir, Md Abdul
Merchant, Andrew
Sadeque, Abdus
Tanveer, Mohsin
Adhikari, Kedar Nath
Huang, Liping
author_sort Muktadir, Md Abdul
collection PubMed
description Identification and validation of biomarkers and bioindicators to select genotypes with superior tolerance to water deficit (WD) under field conditions are paramount to plant breeding programs. However, the co-occurrence of different abiotic stresses such as WD, heat, and radiation makes it difficult to develop generalized protocols to monitor the physiological health of the plant system. The study assessed the most abundant carbohydrates and sugar alcohols in five faba bean (Vicia faba) genotypes under field conditions and the abundance of naturally occurring carbon isotopes in bulk leaf material to predict water use efficiency (WUE). Plant water status and biomass accumulation were also assessed. Among the accumulated sugars, inter-specific variation in glucose was most prevalent and was found at a higher concentration (8.52 mg g(−1) leaf) in rainfed trial. myo-Inositol concentrations followed that of glucose accumulation in that the rainfed trial had higher amounts compared to the irrigated trial. WUE calculated from carbon isotope abundance was consistently offset with measured WUE from measurements of leaf gas exchange. All genotypes demonstrated significant relationships between predicted and measured WUE (p < 0.05) apart from control variety PBA Warda. Thus, bulk leaf-level carbon isotope abundance can be used to calculate WUE and used as an effective selection criterion for improving WUE in faba bean breeding programs under field conditions.
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spelling pubmed-95235852022-10-01 Carbon isotope and soluble metabolites reflect physiological status among contrasting faba bean genotypes in response to water deficit Muktadir, Md Abdul Merchant, Andrew Sadeque, Abdus Tanveer, Mohsin Adhikari, Kedar Nath Huang, Liping Front Plant Sci Plant Science Identification and validation of biomarkers and bioindicators to select genotypes with superior tolerance to water deficit (WD) under field conditions are paramount to plant breeding programs. However, the co-occurrence of different abiotic stresses such as WD, heat, and radiation makes it difficult to develop generalized protocols to monitor the physiological health of the plant system. The study assessed the most abundant carbohydrates and sugar alcohols in five faba bean (Vicia faba) genotypes under field conditions and the abundance of naturally occurring carbon isotopes in bulk leaf material to predict water use efficiency (WUE). Plant water status and biomass accumulation were also assessed. Among the accumulated sugars, inter-specific variation in glucose was most prevalent and was found at a higher concentration (8.52 mg g(−1) leaf) in rainfed trial. myo-Inositol concentrations followed that of glucose accumulation in that the rainfed trial had higher amounts compared to the irrigated trial. WUE calculated from carbon isotope abundance was consistently offset with measured WUE from measurements of leaf gas exchange. All genotypes demonstrated significant relationships between predicted and measured WUE (p < 0.05) apart from control variety PBA Warda. Thus, bulk leaf-level carbon isotope abundance can be used to calculate WUE and used as an effective selection criterion for improving WUE in faba bean breeding programs under field conditions. Frontiers Media S.A. 2022-09-16 /pmc/articles/PMC9523585/ /pubmed/36186012 http://dx.doi.org/10.3389/fpls.2022.955406 Text en Copyright © 2022 Muktadir, Merchant, Sadeque, Tanveer, Adhikari and Huang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Muktadir, Md Abdul
Merchant, Andrew
Sadeque, Abdus
Tanveer, Mohsin
Adhikari, Kedar Nath
Huang, Liping
Carbon isotope and soluble metabolites reflect physiological status among contrasting faba bean genotypes in response to water deficit
title Carbon isotope and soluble metabolites reflect physiological status among contrasting faba bean genotypes in response to water deficit
title_full Carbon isotope and soluble metabolites reflect physiological status among contrasting faba bean genotypes in response to water deficit
title_fullStr Carbon isotope and soluble metabolites reflect physiological status among contrasting faba bean genotypes in response to water deficit
title_full_unstemmed Carbon isotope and soluble metabolites reflect physiological status among contrasting faba bean genotypes in response to water deficit
title_short Carbon isotope and soluble metabolites reflect physiological status among contrasting faba bean genotypes in response to water deficit
title_sort carbon isotope and soluble metabolites reflect physiological status among contrasting faba bean genotypes in response to water deficit
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9523585/
https://www.ncbi.nlm.nih.gov/pubmed/36186012
http://dx.doi.org/10.3389/fpls.2022.955406
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