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Inhibitory activity of a sulfated oligo-porphyran from Pyropia yezoensis against SARS-CoV-2
COVID-19 caused by SARS-CoV-2 has spread around the world at an unprecedented rate. A more homogeneous oligo-porphyran with mean molecular weight of 2.1 kD, named OP145, was separated from Pyropia yezoensis. NMR analysis showed OP145 was mainly composed of →3)-β-d-Gal-(1 → 4)-α-l-Gal (6S) repeating...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Ltd.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9523908/ https://www.ncbi.nlm.nih.gov/pubmed/36876788 http://dx.doi.org/10.1016/j.carbpol.2022.120173 |
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author | Geng, Lihua Zhang, Quanbin Suo, Qishan Wang, Jing Wang, Yingxia Wang, Cong Wu, Ning |
author_facet | Geng, Lihua Zhang, Quanbin Suo, Qishan Wang, Jing Wang, Yingxia Wang, Cong Wu, Ning |
author_sort | Geng, Lihua |
collection | PubMed |
description | COVID-19 caused by SARS-CoV-2 has spread around the world at an unprecedented rate. A more homogeneous oligo-porphyran with mean molecular weight of 2.1 kD, named OP145, was separated from Pyropia yezoensis. NMR analysis showed OP145 was mainly composed of →3)-β-d-Gal-(1 → 4)-α-l-Gal (6S) repeating units with few replacement of 3,6-anhydride, and the molar ratio was 1:0.85:0.11. MALDI-TOF MS revealed OP145 contained mainly tetrasulfate-oligogalactan with Dp range from 4 to 10 and with no more than two 3,6-anhydro-α-l-Gal replacement. The inhibitory activity of OP145 against SARS-CoV-2 was investigated in vitro and in silico. OP145 could bind to Spike glycoprotein (S-protein) through SPR result, and pseudovirus tests confirmed that OP145 could inhibite the infection with an EC50 of 37.52 μg/mL. Molecular docking simulated the interaction between the main component of OP145 and S-protein. All the results indicated that OP145 had the potency to treat and prevent COVID-19. |
format | Online Article Text |
id | pubmed-9523908 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95239082022-09-30 Inhibitory activity of a sulfated oligo-porphyran from Pyropia yezoensis against SARS-CoV-2 Geng, Lihua Zhang, Quanbin Suo, Qishan Wang, Jing Wang, Yingxia Wang, Cong Wu, Ning Carbohydr Polym Article COVID-19 caused by SARS-CoV-2 has spread around the world at an unprecedented rate. A more homogeneous oligo-porphyran with mean molecular weight of 2.1 kD, named OP145, was separated from Pyropia yezoensis. NMR analysis showed OP145 was mainly composed of →3)-β-d-Gal-(1 → 4)-α-l-Gal (6S) repeating units with few replacement of 3,6-anhydride, and the molar ratio was 1:0.85:0.11. MALDI-TOF MS revealed OP145 contained mainly tetrasulfate-oligogalactan with Dp range from 4 to 10 and with no more than two 3,6-anhydro-α-l-Gal replacement. The inhibitory activity of OP145 against SARS-CoV-2 was investigated in vitro and in silico. OP145 could bind to Spike glycoprotein (S-protein) through SPR result, and pseudovirus tests confirmed that OP145 could inhibite the infection with an EC50 of 37.52 μg/mL. Molecular docking simulated the interaction between the main component of OP145 and S-protein. All the results indicated that OP145 had the potency to treat and prevent COVID-19. Elsevier Ltd. 2023-01-01 2022-09-30 /pmc/articles/PMC9523908/ /pubmed/36876788 http://dx.doi.org/10.1016/j.carbpol.2022.120173 Text en © 2022 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Geng, Lihua Zhang, Quanbin Suo, Qishan Wang, Jing Wang, Yingxia Wang, Cong Wu, Ning Inhibitory activity of a sulfated oligo-porphyran from Pyropia yezoensis against SARS-CoV-2 |
title | Inhibitory activity of a sulfated oligo-porphyran from Pyropia yezoensis against SARS-CoV-2 |
title_full | Inhibitory activity of a sulfated oligo-porphyran from Pyropia yezoensis against SARS-CoV-2 |
title_fullStr | Inhibitory activity of a sulfated oligo-porphyran from Pyropia yezoensis against SARS-CoV-2 |
title_full_unstemmed | Inhibitory activity of a sulfated oligo-porphyran from Pyropia yezoensis against SARS-CoV-2 |
title_short | Inhibitory activity of a sulfated oligo-porphyran from Pyropia yezoensis against SARS-CoV-2 |
title_sort | inhibitory activity of a sulfated oligo-porphyran from pyropia yezoensis against sars-cov-2 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9523908/ https://www.ncbi.nlm.nih.gov/pubmed/36876788 http://dx.doi.org/10.1016/j.carbpol.2022.120173 |
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