A conserved transcriptional fingerprint of multi-neurotransmitter neurons necessary for social behavior

BACKGROUND: An essential determinant of a neuron’s functionality is its neurotransmitter phenotype. We previously identified a defined subpopulation of cholinergic neurons required for social orienting behavior in zebrafish. RESULTS: We transcriptionally profiled these neurons and discovered that th...

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Detalles Bibliográficos
Autores principales: Ncube, Denver, Tallafuss, Alexandra, Serafin, Jen, Bruckner, Joseph, Farnsworth, Dylan R., Miller, Adam C., Eisen, Judith S., Washbourne, Philip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9523972/
https://www.ncbi.nlm.nih.gov/pubmed/36175871
http://dx.doi.org/10.1186/s12864-022-08879-w
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author Ncube, Denver
Tallafuss, Alexandra
Serafin, Jen
Bruckner, Joseph
Farnsworth, Dylan R.
Miller, Adam C.
Eisen, Judith S.
Washbourne, Philip
author_facet Ncube, Denver
Tallafuss, Alexandra
Serafin, Jen
Bruckner, Joseph
Farnsworth, Dylan R.
Miller, Adam C.
Eisen, Judith S.
Washbourne, Philip
author_sort Ncube, Denver
collection PubMed
description BACKGROUND: An essential determinant of a neuron’s functionality is its neurotransmitter phenotype. We previously identified a defined subpopulation of cholinergic neurons required for social orienting behavior in zebrafish. RESULTS: We transcriptionally profiled these neurons and discovered that they are capable of synthesizing both acetylcholine and GABA. We also established a constellation of transcription factors and neurotransmitter markers that can be used as a “transcriptomic fingerprint” to recognize a homologous neuronal population in another vertebrate. CONCLUSION: Our results suggest that this transcriptomic fingerprint and the cholinergic-GABAergic neuronal subtype that it defines are evolutionarily conserved. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-022-08879-w.
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spelling pubmed-95239722022-10-01 A conserved transcriptional fingerprint of multi-neurotransmitter neurons necessary for social behavior Ncube, Denver Tallafuss, Alexandra Serafin, Jen Bruckner, Joseph Farnsworth, Dylan R. Miller, Adam C. Eisen, Judith S. Washbourne, Philip BMC Genomics Research BACKGROUND: An essential determinant of a neuron’s functionality is its neurotransmitter phenotype. We previously identified a defined subpopulation of cholinergic neurons required for social orienting behavior in zebrafish. RESULTS: We transcriptionally profiled these neurons and discovered that they are capable of synthesizing both acetylcholine and GABA. We also established a constellation of transcription factors and neurotransmitter markers that can be used as a “transcriptomic fingerprint” to recognize a homologous neuronal population in another vertebrate. CONCLUSION: Our results suggest that this transcriptomic fingerprint and the cholinergic-GABAergic neuronal subtype that it defines are evolutionarily conserved. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-022-08879-w. BioMed Central 2022-09-29 /pmc/articles/PMC9523972/ /pubmed/36175871 http://dx.doi.org/10.1186/s12864-022-08879-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Ncube, Denver
Tallafuss, Alexandra
Serafin, Jen
Bruckner, Joseph
Farnsworth, Dylan R.
Miller, Adam C.
Eisen, Judith S.
Washbourne, Philip
A conserved transcriptional fingerprint of multi-neurotransmitter neurons necessary for social behavior
title A conserved transcriptional fingerprint of multi-neurotransmitter neurons necessary for social behavior
title_full A conserved transcriptional fingerprint of multi-neurotransmitter neurons necessary for social behavior
title_fullStr A conserved transcriptional fingerprint of multi-neurotransmitter neurons necessary for social behavior
title_full_unstemmed A conserved transcriptional fingerprint of multi-neurotransmitter neurons necessary for social behavior
title_short A conserved transcriptional fingerprint of multi-neurotransmitter neurons necessary for social behavior
title_sort conserved transcriptional fingerprint of multi-neurotransmitter neurons necessary for social behavior
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9523972/
https://www.ncbi.nlm.nih.gov/pubmed/36175871
http://dx.doi.org/10.1186/s12864-022-08879-w
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