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The cell cortex-localized protein CHDP-1 is required for dendritic development and transport in C. elegans neurons
Cortical actin, a thin layer of actin network underneath the plasma membranes, plays critical roles in numerous processes, such as cell morphogenesis and migration. Neurons often grow highly branched dendrite morphologies, which is crucial for neural circuit assembly. It is still poorly understood h...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9524629/ https://www.ncbi.nlm.nih.gov/pubmed/36126047 http://dx.doi.org/10.1371/journal.pgen.1010381 |
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author | Zhao, Ting Guan, Liying Ma, Xuehua Chen, Baohui Ding, Mei Zou, Wei |
author_facet | Zhao, Ting Guan, Liying Ma, Xuehua Chen, Baohui Ding, Mei Zou, Wei |
author_sort | Zhao, Ting |
collection | PubMed |
description | Cortical actin, a thin layer of actin network underneath the plasma membranes, plays critical roles in numerous processes, such as cell morphogenesis and migration. Neurons often grow highly branched dendrite morphologies, which is crucial for neural circuit assembly. It is still poorly understood how cortical actin assembly is controlled in dendrites and whether it is critical for dendrite development, maintenance and function. In the present study, we find that knock-out of C. elegans chdp-1, which encodes a cell cortex-localized protein, causes dendrite formation defects in the larval stages and spontaneous dendrite degeneration in adults. Actin assembly in the dendritic growth cones is significantly reduced in the chdp-1 mutants. PVD neurons sense muscle contraction and act as proprioceptors. Loss of chdp-1 abolishes proprioception, which can be rescued by expressing CHDP-1 in the PVD neurons. In the high-ordered branches, loss of chdp-1 also severely affects the microtubule cytoskeleton assembly, intracellular organelle transport and neuropeptide secretion. Interestingly, knock-out of sax-1, which encodes an evolutionary conserved serine/threonine protein kinase, suppresses the defects mentioned above in chdp-1 mutants. Thus, our findings suggest that CHDP-1 and SAX-1 function in an opposing manner in the multi-dendritic neurons to modulate cortical actin assembly, which is critical for dendrite development, maintenance and function. |
format | Online Article Text |
id | pubmed-9524629 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-95246292022-10-01 The cell cortex-localized protein CHDP-1 is required for dendritic development and transport in C. elegans neurons Zhao, Ting Guan, Liying Ma, Xuehua Chen, Baohui Ding, Mei Zou, Wei PLoS Genet Research Article Cortical actin, a thin layer of actin network underneath the plasma membranes, plays critical roles in numerous processes, such as cell morphogenesis and migration. Neurons often grow highly branched dendrite morphologies, which is crucial for neural circuit assembly. It is still poorly understood how cortical actin assembly is controlled in dendrites and whether it is critical for dendrite development, maintenance and function. In the present study, we find that knock-out of C. elegans chdp-1, which encodes a cell cortex-localized protein, causes dendrite formation defects in the larval stages and spontaneous dendrite degeneration in adults. Actin assembly in the dendritic growth cones is significantly reduced in the chdp-1 mutants. PVD neurons sense muscle contraction and act as proprioceptors. Loss of chdp-1 abolishes proprioception, which can be rescued by expressing CHDP-1 in the PVD neurons. In the high-ordered branches, loss of chdp-1 also severely affects the microtubule cytoskeleton assembly, intracellular organelle transport and neuropeptide secretion. Interestingly, knock-out of sax-1, which encodes an evolutionary conserved serine/threonine protein kinase, suppresses the defects mentioned above in chdp-1 mutants. Thus, our findings suggest that CHDP-1 and SAX-1 function in an opposing manner in the multi-dendritic neurons to modulate cortical actin assembly, which is critical for dendrite development, maintenance and function. Public Library of Science 2022-09-20 /pmc/articles/PMC9524629/ /pubmed/36126047 http://dx.doi.org/10.1371/journal.pgen.1010381 Text en © 2022 Zhao et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zhao, Ting Guan, Liying Ma, Xuehua Chen, Baohui Ding, Mei Zou, Wei The cell cortex-localized protein CHDP-1 is required for dendritic development and transport in C. elegans neurons |
title | The cell cortex-localized protein CHDP-1 is required for dendritic development and transport in C. elegans neurons |
title_full | The cell cortex-localized protein CHDP-1 is required for dendritic development and transport in C. elegans neurons |
title_fullStr | The cell cortex-localized protein CHDP-1 is required for dendritic development and transport in C. elegans neurons |
title_full_unstemmed | The cell cortex-localized protein CHDP-1 is required for dendritic development and transport in C. elegans neurons |
title_short | The cell cortex-localized protein CHDP-1 is required for dendritic development and transport in C. elegans neurons |
title_sort | cell cortex-localized protein chdp-1 is required for dendritic development and transport in c. elegans neurons |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9524629/ https://www.ncbi.nlm.nih.gov/pubmed/36126047 http://dx.doi.org/10.1371/journal.pgen.1010381 |
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