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Molecular Characterization Identifies Upstream Presence of IS Aba1 to OXA Carbapenemase Genes in Carbapenem-Resistant Acinetobacter baumannii
Background Evaluating the expression pattern of oxacillinases (OXA) carbapenemases is essential to understand the prevalence and spread of carbapenem resistance Acinetobacter baumannii . Objectives The aim of the study is to evaluate the presence of OXA carbapenemase genes and IS Aba1 upstream to...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Thieme Medical and Scientific Publishers Pvt. Ltd.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9525184/ https://www.ncbi.nlm.nih.gov/pubmed/36186260 http://dx.doi.org/10.1055/s-0041-1732809 |
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author | Gandham, Nageswari Gupta, Neetu Vyawahare, Chanda Mirza, Shahzad Beg Misra, Rabindra Nath |
author_facet | Gandham, Nageswari Gupta, Neetu Vyawahare, Chanda Mirza, Shahzad Beg Misra, Rabindra Nath |
author_sort | Gandham, Nageswari |
collection | PubMed |
description | Background Evaluating the expression pattern of oxacillinases (OXA) carbapenemases is essential to understand the prevalence and spread of carbapenem resistance Acinetobacter baumannii . Objectives The aim of the study is to evaluate the presence of OXA carbapenemase genes and IS Aba1 upstream to these genes in carbapenem-resistant A. baumannii clinical isolates. Materials and Methods A. baumannii isolated from clinical samples were phenotypically identified and antibiotics sensitivity was performed. Multiplex polymerase chain reaction (PCR) was used to detect OXA51-like gene, OXA carbapenemases genes (OXA-23-like, OXA-24-like, and OXA-58-like), and IS Aba1 in carbapenem-resistant isolates. Results Out of 55 Acinetobacter isolates, 54 were confirmed as A. baumannii by PCR. Bla (OXA-23) -like gene was observed in 51 isolates of A. baumannii and none of the isolates showed the presence of bla (OXA-24) -like and bla (OXA-58) -like genes. Presence of IS Aba1 upstream to OXA-23-like gene, OXA-51-like gene, and both OXA-51-like/OXA-23-like genes was observed in 51, 7, and 4 A. baumannii isolates, respectively. Conclusion The genetic pattern of carbapenem-resistant A. baumannii isolated in this study was unique, which should be factored for clinical protocols to manage infections caused by emerging resistant strains of A. baumannii . |
format | Online Article Text |
id | pubmed-9525184 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Thieme Medical and Scientific Publishers Pvt. Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95251842022-10-01 Molecular Characterization Identifies Upstream Presence of IS Aba1 to OXA Carbapenemase Genes in Carbapenem-Resistant Acinetobacter baumannii Gandham, Nageswari Gupta, Neetu Vyawahare, Chanda Mirza, Shahzad Beg Misra, Rabindra Nath J Lab Physicians Background Evaluating the expression pattern of oxacillinases (OXA) carbapenemases is essential to understand the prevalence and spread of carbapenem resistance Acinetobacter baumannii . Objectives The aim of the study is to evaluate the presence of OXA carbapenemase genes and IS Aba1 upstream to these genes in carbapenem-resistant A. baumannii clinical isolates. Materials and Methods A. baumannii isolated from clinical samples were phenotypically identified and antibiotics sensitivity was performed. Multiplex polymerase chain reaction (PCR) was used to detect OXA51-like gene, OXA carbapenemases genes (OXA-23-like, OXA-24-like, and OXA-58-like), and IS Aba1 in carbapenem-resistant isolates. Results Out of 55 Acinetobacter isolates, 54 were confirmed as A. baumannii by PCR. Bla (OXA-23) -like gene was observed in 51 isolates of A. baumannii and none of the isolates showed the presence of bla (OXA-24) -like and bla (OXA-58) -like genes. Presence of IS Aba1 upstream to OXA-23-like gene, OXA-51-like gene, and both OXA-51-like/OXA-23-like genes was observed in 51, 7, and 4 A. baumannii isolates, respectively. Conclusion The genetic pattern of carbapenem-resistant A. baumannii isolated in this study was unique, which should be factored for clinical protocols to manage infections caused by emerging resistant strains of A. baumannii . Thieme Medical and Scientific Publishers Pvt. Ltd. 2021-08-31 /pmc/articles/PMC9525184/ /pubmed/36186260 http://dx.doi.org/10.1055/s-0041-1732809 Text en The Indian Association of Laboratory Physicians. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. ( https://creativecommons.org/licenses/by-nc-nd/4.0/ ) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited. |
spellingShingle | Gandham, Nageswari Gupta, Neetu Vyawahare, Chanda Mirza, Shahzad Beg Misra, Rabindra Nath Molecular Characterization Identifies Upstream Presence of IS Aba1 to OXA Carbapenemase Genes in Carbapenem-Resistant Acinetobacter baumannii |
title |
Molecular Characterization Identifies Upstream Presence of IS
Aba1
to
OXA
Carbapenemase Genes in Carbapenem-Resistant
Acinetobacter baumannii
|
title_full |
Molecular Characterization Identifies Upstream Presence of IS
Aba1
to
OXA
Carbapenemase Genes in Carbapenem-Resistant
Acinetobacter baumannii
|
title_fullStr |
Molecular Characterization Identifies Upstream Presence of IS
Aba1
to
OXA
Carbapenemase Genes in Carbapenem-Resistant
Acinetobacter baumannii
|
title_full_unstemmed |
Molecular Characterization Identifies Upstream Presence of IS
Aba1
to
OXA
Carbapenemase Genes in Carbapenem-Resistant
Acinetobacter baumannii
|
title_short |
Molecular Characterization Identifies Upstream Presence of IS
Aba1
to
OXA
Carbapenemase Genes in Carbapenem-Resistant
Acinetobacter baumannii
|
title_sort | molecular characterization identifies upstream presence of is
aba1
to
oxa
carbapenemase genes in carbapenem-resistant
acinetobacter baumannii |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9525184/ https://www.ncbi.nlm.nih.gov/pubmed/36186260 http://dx.doi.org/10.1055/s-0041-1732809 |
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