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CXCL13 is expressed in a subpopulation of neuroendocrine cells in the murine trachea and lung
The conducting airways are lined by distinct cell types, comprising basal, secretory, ciliated, and rare cells, including ionocytes, solitary cholinergic chemosensory cells, and solitary and clustered (neuroepithelial bodies) neuroendocrine cells. Airway neuroendocrine cells are in clinical focus si...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9525416/ https://www.ncbi.nlm.nih.gov/pubmed/34762185 http://dx.doi.org/10.1007/s00441-021-03552-2 |
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author | Mahmoud, Wafaa Perniss, Alexander Poharkar, Krupali Soultanova, Aichurek Pfeil, Uwe Hoek, Andreas Bhushan, Sudhanshu Hain, Torsten Gärtner, Ulrich Kummer, Wolfgang |
author_facet | Mahmoud, Wafaa Perniss, Alexander Poharkar, Krupali Soultanova, Aichurek Pfeil, Uwe Hoek, Andreas Bhushan, Sudhanshu Hain, Torsten Gärtner, Ulrich Kummer, Wolfgang |
author_sort | Mahmoud, Wafaa |
collection | PubMed |
description | The conducting airways are lined by distinct cell types, comprising basal, secretory, ciliated, and rare cells, including ionocytes, solitary cholinergic chemosensory cells, and solitary and clustered (neuroepithelial bodies) neuroendocrine cells. Airway neuroendocrine cells are in clinical focus since they can give rise to small cell lung cancer. They have been implicated in diverse functions including mechanosensation, chemosensation, and regeneration, and were recently identified as regulators of type 2 immune responses via the release of the neuropeptide calcitonin gene-related peptide (CGRP). We here assessed the expression of the chemokine CXCL13 (B cell attracting chemokine) by these cells by RT-PCR, in silico analysis of publicly available sequencing data sets, immunohistochemistry, and immuno-electron microscopy. We identify a phenotype of neuroendocrine cells in the naïve mouse, producing the chemokine CXCL13 predominantly in solitary neuroendocrine cells of the tracheal epithelium (approx. 70% CXCL13(+)) and, to a lesser extent, in the solitary neuroendocrine cells and neuroepithelial bodies of the intrapulmonary bronchial epithelium (< 10% CXCL13(+)). In silico analysis of published sequencing data of murine tracheal epithelial cells was consistent with the results obtained by immunohistochemistry as it revealed that neuroendocrine cells are the major source of Cxcl13-mRNA, which was expressed by 68–79% of neuroendocrine cells. An unbiased scRNA-seq data analysis of overall gene expression did not yield subclusters of neuroendocrine cells. Our observation demonstrates phenotypic heterogeneity of airway neuroendocrine cells and points towards a putative immunoregulatory role of these cells in bronchial-associated lymphoid tissue formation and B cell homeostasis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00441-021-03552-2. |
format | Online Article Text |
id | pubmed-9525416 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-95254162022-10-02 CXCL13 is expressed in a subpopulation of neuroendocrine cells in the murine trachea and lung Mahmoud, Wafaa Perniss, Alexander Poharkar, Krupali Soultanova, Aichurek Pfeil, Uwe Hoek, Andreas Bhushan, Sudhanshu Hain, Torsten Gärtner, Ulrich Kummer, Wolfgang Cell Tissue Res Regular Article The conducting airways are lined by distinct cell types, comprising basal, secretory, ciliated, and rare cells, including ionocytes, solitary cholinergic chemosensory cells, and solitary and clustered (neuroepithelial bodies) neuroendocrine cells. Airway neuroendocrine cells are in clinical focus since they can give rise to small cell lung cancer. They have been implicated in diverse functions including mechanosensation, chemosensation, and regeneration, and were recently identified as regulators of type 2 immune responses via the release of the neuropeptide calcitonin gene-related peptide (CGRP). We here assessed the expression of the chemokine CXCL13 (B cell attracting chemokine) by these cells by RT-PCR, in silico analysis of publicly available sequencing data sets, immunohistochemistry, and immuno-electron microscopy. We identify a phenotype of neuroendocrine cells in the naïve mouse, producing the chemokine CXCL13 predominantly in solitary neuroendocrine cells of the tracheal epithelium (approx. 70% CXCL13(+)) and, to a lesser extent, in the solitary neuroendocrine cells and neuroepithelial bodies of the intrapulmonary bronchial epithelium (< 10% CXCL13(+)). In silico analysis of published sequencing data of murine tracheal epithelial cells was consistent with the results obtained by immunohistochemistry as it revealed that neuroendocrine cells are the major source of Cxcl13-mRNA, which was expressed by 68–79% of neuroendocrine cells. An unbiased scRNA-seq data analysis of overall gene expression did not yield subclusters of neuroendocrine cells. Our observation demonstrates phenotypic heterogeneity of airway neuroendocrine cells and points towards a putative immunoregulatory role of these cells in bronchial-associated lymphoid tissue formation and B cell homeostasis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00441-021-03552-2. Springer Berlin Heidelberg 2021-11-11 2022 /pmc/articles/PMC9525416/ /pubmed/34762185 http://dx.doi.org/10.1007/s00441-021-03552-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Regular Article Mahmoud, Wafaa Perniss, Alexander Poharkar, Krupali Soultanova, Aichurek Pfeil, Uwe Hoek, Andreas Bhushan, Sudhanshu Hain, Torsten Gärtner, Ulrich Kummer, Wolfgang CXCL13 is expressed in a subpopulation of neuroendocrine cells in the murine trachea and lung |
title | CXCL13 is expressed in a subpopulation of neuroendocrine cells in the murine trachea and lung |
title_full | CXCL13 is expressed in a subpopulation of neuroendocrine cells in the murine trachea and lung |
title_fullStr | CXCL13 is expressed in a subpopulation of neuroendocrine cells in the murine trachea and lung |
title_full_unstemmed | CXCL13 is expressed in a subpopulation of neuroendocrine cells in the murine trachea and lung |
title_short | CXCL13 is expressed in a subpopulation of neuroendocrine cells in the murine trachea and lung |
title_sort | cxcl13 is expressed in a subpopulation of neuroendocrine cells in the murine trachea and lung |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9525416/ https://www.ncbi.nlm.nih.gov/pubmed/34762185 http://dx.doi.org/10.1007/s00441-021-03552-2 |
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