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Synthetic torpor protects rats from exposure to accelerated heavy ions

Hibernation or torpor is considered a possible tool to protect astronauts from the deleterious effects of space radiation that contains high-energy heavy ions. We induced synthetic torpor in rats by injecting adenosine 5′-monophosphate monohydrate (5′-AMP) i.p. and maintaining in low ambient tempera...

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Autores principales: Puspitasari, Anggraeini, Squarcio, Fabio, Quartieri, Martina, Totis, Cristina, Hitrec, Timna, Takahashi, Akihisa, Yoshida, Yukari, Hanamura, Kenji, Yako, Tomoko, Cerri, Matteo, Simoniello, Palma, Durante, Marco, Tinganelli, Walter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9525701/
https://www.ncbi.nlm.nih.gov/pubmed/36180516
http://dx.doi.org/10.1038/s41598-022-20382-6
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author Puspitasari, Anggraeini
Squarcio, Fabio
Quartieri, Martina
Totis, Cristina
Hitrec, Timna
Takahashi, Akihisa
Yoshida, Yukari
Hanamura, Kenji
Yako, Tomoko
Cerri, Matteo
Simoniello, Palma
Durante, Marco
Tinganelli, Walter
author_facet Puspitasari, Anggraeini
Squarcio, Fabio
Quartieri, Martina
Totis, Cristina
Hitrec, Timna
Takahashi, Akihisa
Yoshida, Yukari
Hanamura, Kenji
Yako, Tomoko
Cerri, Matteo
Simoniello, Palma
Durante, Marco
Tinganelli, Walter
author_sort Puspitasari, Anggraeini
collection PubMed
description Hibernation or torpor is considered a possible tool to protect astronauts from the deleterious effects of space radiation that contains high-energy heavy ions. We induced synthetic torpor in rats by injecting adenosine 5′-monophosphate monohydrate (5′-AMP) i.p. and maintaining in low ambient temperature room (+ 16 °C) for 6 h immediately after total body irradiation (TBI) with accelerated carbon ions (C-ions). The 5′-AMP treatment in combination with low ambient temperature reduced skin temperature and increased survival following 8 Gy C-ion irradiation compared to saline-injected animals. Analysis of the histology of the brain, liver and lungs showed that 5′-AMP treatment following 2 Gy TBI reduced activated microglia, Iba1 positive cells in the brain, apoptotic cells in the liver, and damage to the lungs, suggesting that synthetic torpor spares tissues from energetic ion radiation. The application of 5′-AMP in combination with either hypoxia or low temperature environment for six hours following irradiation of rat retinal pigment epithelial cells delays DNA repair and suppresses the radiation-induced mitotic catastrophe compared to control cells. We conclude that synthetic torpor protects animals from cosmic ray-simulated radiation and the mechanism involves both hypothermia and hypoxia.
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spelling pubmed-95257012022-10-02 Synthetic torpor protects rats from exposure to accelerated heavy ions Puspitasari, Anggraeini Squarcio, Fabio Quartieri, Martina Totis, Cristina Hitrec, Timna Takahashi, Akihisa Yoshida, Yukari Hanamura, Kenji Yako, Tomoko Cerri, Matteo Simoniello, Palma Durante, Marco Tinganelli, Walter Sci Rep Article Hibernation or torpor is considered a possible tool to protect astronauts from the deleterious effects of space radiation that contains high-energy heavy ions. We induced synthetic torpor in rats by injecting adenosine 5′-monophosphate monohydrate (5′-AMP) i.p. and maintaining in low ambient temperature room (+ 16 °C) for 6 h immediately after total body irradiation (TBI) with accelerated carbon ions (C-ions). The 5′-AMP treatment in combination with low ambient temperature reduced skin temperature and increased survival following 8 Gy C-ion irradiation compared to saline-injected animals. Analysis of the histology of the brain, liver and lungs showed that 5′-AMP treatment following 2 Gy TBI reduced activated microglia, Iba1 positive cells in the brain, apoptotic cells in the liver, and damage to the lungs, suggesting that synthetic torpor spares tissues from energetic ion radiation. The application of 5′-AMP in combination with either hypoxia or low temperature environment for six hours following irradiation of rat retinal pigment epithelial cells delays DNA repair and suppresses the radiation-induced mitotic catastrophe compared to control cells. We conclude that synthetic torpor protects animals from cosmic ray-simulated radiation and the mechanism involves both hypothermia and hypoxia. Nature Publishing Group UK 2022-09-30 /pmc/articles/PMC9525701/ /pubmed/36180516 http://dx.doi.org/10.1038/s41598-022-20382-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Puspitasari, Anggraeini
Squarcio, Fabio
Quartieri, Martina
Totis, Cristina
Hitrec, Timna
Takahashi, Akihisa
Yoshida, Yukari
Hanamura, Kenji
Yako, Tomoko
Cerri, Matteo
Simoniello, Palma
Durante, Marco
Tinganelli, Walter
Synthetic torpor protects rats from exposure to accelerated heavy ions
title Synthetic torpor protects rats from exposure to accelerated heavy ions
title_full Synthetic torpor protects rats from exposure to accelerated heavy ions
title_fullStr Synthetic torpor protects rats from exposure to accelerated heavy ions
title_full_unstemmed Synthetic torpor protects rats from exposure to accelerated heavy ions
title_short Synthetic torpor protects rats from exposure to accelerated heavy ions
title_sort synthetic torpor protects rats from exposure to accelerated heavy ions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9525701/
https://www.ncbi.nlm.nih.gov/pubmed/36180516
http://dx.doi.org/10.1038/s41598-022-20382-6
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