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Blockade of vasopressin receptors reduces the threshold pressure of micturition reflex in female rats

The mechanisms involved in urinary bladder control are not fully understood, but it is well accepted that a complex central network is involved in micturition control. The micturition reflex can be modulated by direct cortical influence through facilitatory and inhibitory mechanisms. In addition, hu...

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Autores principales: Neri, Luciana S.S., de Carvalho, Rodrigo P., Daiuto, Sergio A., Vale, Bárbara do, Cafarchio, Eduardo M., Aronsson, Patrik, Sato, Monica A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9525731/
https://www.ncbi.nlm.nih.gov/pubmed/36193514
http://dx.doi.org/10.1016/j.crphys.2022.09.004
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author Neri, Luciana S.S.
de Carvalho, Rodrigo P.
Daiuto, Sergio A.
Vale, Bárbara do
Cafarchio, Eduardo M.
Aronsson, Patrik
Sato, Monica A.
author_facet Neri, Luciana S.S.
de Carvalho, Rodrigo P.
Daiuto, Sergio A.
Vale, Bárbara do
Cafarchio, Eduardo M.
Aronsson, Patrik
Sato, Monica A.
author_sort Neri, Luciana S.S.
collection PubMed
description The mechanisms involved in urinary bladder control are not fully understood, but it is well accepted that a complex central network is involved in micturition control. The micturition reflex can be modulated by direct cortical influence through facilitatory and inhibitory mechanisms. In addition, humoral mechanisms are involved in the bladder control. Vasopressin increases bladder contraction and intravesical pressure. This study sought to investigate the effect of intravenous injections of vasopressin receptor antagonists on cystometric parameters in anesthetized female rats. Isoflurane anesthetized adult female Wistar rats underwent femoral artery and vein cannulation for arterial pressure (AP) and heart rate (HR) recordings, and infusion of drugs, respectively. The bladder was also cannulated for intravesical pressure (IP) recordings and infusion of saline (10 mL/h) for cystometric evaluation. After baseline AP, HR and IP recordings, saline (vehicle, 1 mL/kg), V1a (5 μg/kg) or V2 receptor antagonist (5 μg/kg) was injected i.v. and after 25 min the cystometry was carried out. Neither saline nor V1a or V2 receptor blockade evoked any change in AP, HR and IP. Nevertheless, during cystometry, the threshold pressure of the micturition reflex was significantly reduced in rats with V1a (to 19.30 ± 2.39 mmHg) and V2 receptor blockade (to 19.88 ± 2.49 mmHg) compared to the saline group (28.85 ± 2.06 mmHg, p = 0.014). No difference was observed in the other cystometric parameters. Therefore, the data suggest that blockade of V1a and V2 receptors reduces the threshold pressure of the micturition reflex and does not influence other cystometric parameters in anesthetized female Wistar rats.
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spelling pubmed-95257312022-10-02 Blockade of vasopressin receptors reduces the threshold pressure of micturition reflex in female rats Neri, Luciana S.S. de Carvalho, Rodrigo P. Daiuto, Sergio A. Vale, Bárbara do Cafarchio, Eduardo M. Aronsson, Patrik Sato, Monica A. Curr Res Physiol Research Paper The mechanisms involved in urinary bladder control are not fully understood, but it is well accepted that a complex central network is involved in micturition control. The micturition reflex can be modulated by direct cortical influence through facilitatory and inhibitory mechanisms. In addition, humoral mechanisms are involved in the bladder control. Vasopressin increases bladder contraction and intravesical pressure. This study sought to investigate the effect of intravenous injections of vasopressin receptor antagonists on cystometric parameters in anesthetized female rats. Isoflurane anesthetized adult female Wistar rats underwent femoral artery and vein cannulation for arterial pressure (AP) and heart rate (HR) recordings, and infusion of drugs, respectively. The bladder was also cannulated for intravesical pressure (IP) recordings and infusion of saline (10 mL/h) for cystometric evaluation. After baseline AP, HR and IP recordings, saline (vehicle, 1 mL/kg), V1a (5 μg/kg) or V2 receptor antagonist (5 μg/kg) was injected i.v. and after 25 min the cystometry was carried out. Neither saline nor V1a or V2 receptor blockade evoked any change in AP, HR and IP. Nevertheless, during cystometry, the threshold pressure of the micturition reflex was significantly reduced in rats with V1a (to 19.30 ± 2.39 mmHg) and V2 receptor blockade (to 19.88 ± 2.49 mmHg) compared to the saline group (28.85 ± 2.06 mmHg, p = 0.014). No difference was observed in the other cystometric parameters. Therefore, the data suggest that blockade of V1a and V2 receptors reduces the threshold pressure of the micturition reflex and does not influence other cystometric parameters in anesthetized female Wistar rats. Elsevier 2022-09-24 /pmc/articles/PMC9525731/ /pubmed/36193514 http://dx.doi.org/10.1016/j.crphys.2022.09.004 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Paper
Neri, Luciana S.S.
de Carvalho, Rodrigo P.
Daiuto, Sergio A.
Vale, Bárbara do
Cafarchio, Eduardo M.
Aronsson, Patrik
Sato, Monica A.
Blockade of vasopressin receptors reduces the threshold pressure of micturition reflex in female rats
title Blockade of vasopressin receptors reduces the threshold pressure of micturition reflex in female rats
title_full Blockade of vasopressin receptors reduces the threshold pressure of micturition reflex in female rats
title_fullStr Blockade of vasopressin receptors reduces the threshold pressure of micturition reflex in female rats
title_full_unstemmed Blockade of vasopressin receptors reduces the threshold pressure of micturition reflex in female rats
title_short Blockade of vasopressin receptors reduces the threshold pressure of micturition reflex in female rats
title_sort blockade of vasopressin receptors reduces the threshold pressure of micturition reflex in female rats
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9525731/
https://www.ncbi.nlm.nih.gov/pubmed/36193514
http://dx.doi.org/10.1016/j.crphys.2022.09.004
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