Protocol for high-throughput reservoir quantification across global HIV subtypes using a cross-subtype intact proviral DNA assay

The cross-subtype intact proviral DNA assay (CS-IPDA) is a high-throughput method to quantify HIV reservoir size in populations infected with any of the dominant global HIV-1 subtypes. Our protocol includes genomic DNA isolation optimized to minimize DNA shearing, a reference droplet digital PCR (dd...

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Detalles Bibliográficos
Autores principales: Fish, Carolyn S., Cassidy, Noah A.J., Levy, Claire N., Hughes, Sean M., Jerome, Keith R., Overbaugh, Julie, Hladik, Florian, Lehman, Dara A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9525950/
https://www.ncbi.nlm.nih.gov/pubmed/36178789
http://dx.doi.org/10.1016/j.xpro.2022.101681

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