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Upconverting nanoparticle-containing erythrocyte-sized hemoglobin microgels that generate heat, oxygen and reactive oxygen species for suppressing hypoxic tumors

Inspired by erythrocytes that contain oxygen-carrying hemoglobin (Hb) and that exhibit photo-driven activity, we introduce homogenous-sized erythrocyte-like Hb microgel (μGel) systems (5–6 μm) that can (i) emit heat, (ii) supply oxygen, and (iii) generate reactive oxygen species (ROS; (1)O(2)) in re...

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Autores principales: Kim, Hanju, Yoon, Johyun, Kim, Hwang Kyung, Lee, Woo Tak, Nguyen, Nguyen Thi, Le, Xuan Thien, Lee, Eun-Hee, Lee, Eun Seong, Oh, Kyung Taek, Choi, Han-Gon, Youn, Yu Seok
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9526021/
https://www.ncbi.nlm.nih.gov/pubmed/36203958
http://dx.doi.org/10.1016/j.bioactmat.2022.09.020
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author Kim, Hanju
Yoon, Johyun
Kim, Hwang Kyung
Lee, Woo Tak
Nguyen, Nguyen Thi
Le, Xuan Thien
Lee, Eun-Hee
Lee, Eun Seong
Oh, Kyung Taek
Choi, Han-Gon
Youn, Yu Seok
author_facet Kim, Hanju
Yoon, Johyun
Kim, Hwang Kyung
Lee, Woo Tak
Nguyen, Nguyen Thi
Le, Xuan Thien
Lee, Eun-Hee
Lee, Eun Seong
Oh, Kyung Taek
Choi, Han-Gon
Youn, Yu Seok
author_sort Kim, Hanju
collection PubMed
description Inspired by erythrocytes that contain oxygen-carrying hemoglobin (Hb) and that exhibit photo-driven activity, we introduce homogenous-sized erythrocyte-like Hb microgel (μGel) systems (5–6 μm) that can (i) emit heat, (ii) supply oxygen, and (iii) generate reactive oxygen species (ROS; (1)O(2)) in response to near-infrared (NIR) laser irradiation. Hb μGels consist of Hb, bovine serum albumin (BSA), chlorin e6 (Ce6) and erbium@lutetium upconverting nanoparticles (UCNPs; ∼35 nm) that effectively convert 808 nm NIR light to 660 nm visible light. These Hb μGels are capable of releasing oxygen to help generate sufficient reactive oxygen species ((1)O(2)) from UCNPs/Ce6 under severely hypoxic condition upon NIR stimulation for efficient photodynamic activity. Moreover, the Hb μGels emit heat and increase surface temperature due to NIR light absorption by heme (iron protoporphyrin IX) and display photothermal activity. By changing the Hb/UCNP/Ce6 ratio and controlling the amount of NIR laser irradiation, it is possible to formulate bespoke Hb μGels with either photothermal or photodynamic activity or both in the context of combined therapeutic effect. These Hb μGels effectively suppress highly hypoxic 4T1 cell spheroid growth and xenograft mice tumors in vivo.
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spelling pubmed-95260212022-10-05 Upconverting nanoparticle-containing erythrocyte-sized hemoglobin microgels that generate heat, oxygen and reactive oxygen species for suppressing hypoxic tumors Kim, Hanju Yoon, Johyun Kim, Hwang Kyung Lee, Woo Tak Nguyen, Nguyen Thi Le, Xuan Thien Lee, Eun-Hee Lee, Eun Seong Oh, Kyung Taek Choi, Han-Gon Youn, Yu Seok Bioact Mater Article Inspired by erythrocytes that contain oxygen-carrying hemoglobin (Hb) and that exhibit photo-driven activity, we introduce homogenous-sized erythrocyte-like Hb microgel (μGel) systems (5–6 μm) that can (i) emit heat, (ii) supply oxygen, and (iii) generate reactive oxygen species (ROS; (1)O(2)) in response to near-infrared (NIR) laser irradiation. Hb μGels consist of Hb, bovine serum albumin (BSA), chlorin e6 (Ce6) and erbium@lutetium upconverting nanoparticles (UCNPs; ∼35 nm) that effectively convert 808 nm NIR light to 660 nm visible light. These Hb μGels are capable of releasing oxygen to help generate sufficient reactive oxygen species ((1)O(2)) from UCNPs/Ce6 under severely hypoxic condition upon NIR stimulation for efficient photodynamic activity. Moreover, the Hb μGels emit heat and increase surface temperature due to NIR light absorption by heme (iron protoporphyrin IX) and display photothermal activity. By changing the Hb/UCNP/Ce6 ratio and controlling the amount of NIR laser irradiation, it is possible to formulate bespoke Hb μGels with either photothermal or photodynamic activity or both in the context of combined therapeutic effect. These Hb μGels effectively suppress highly hypoxic 4T1 cell spheroid growth and xenograft mice tumors in vivo. KeAi Publishing 2022-09-28 /pmc/articles/PMC9526021/ /pubmed/36203958 http://dx.doi.org/10.1016/j.bioactmat.2022.09.020 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Kim, Hanju
Yoon, Johyun
Kim, Hwang Kyung
Lee, Woo Tak
Nguyen, Nguyen Thi
Le, Xuan Thien
Lee, Eun-Hee
Lee, Eun Seong
Oh, Kyung Taek
Choi, Han-Gon
Youn, Yu Seok
Upconverting nanoparticle-containing erythrocyte-sized hemoglobin microgels that generate heat, oxygen and reactive oxygen species for suppressing hypoxic tumors
title Upconverting nanoparticle-containing erythrocyte-sized hemoglobin microgels that generate heat, oxygen and reactive oxygen species for suppressing hypoxic tumors
title_full Upconverting nanoparticle-containing erythrocyte-sized hemoglobin microgels that generate heat, oxygen and reactive oxygen species for suppressing hypoxic tumors
title_fullStr Upconverting nanoparticle-containing erythrocyte-sized hemoglobin microgels that generate heat, oxygen and reactive oxygen species for suppressing hypoxic tumors
title_full_unstemmed Upconverting nanoparticle-containing erythrocyte-sized hemoglobin microgels that generate heat, oxygen and reactive oxygen species for suppressing hypoxic tumors
title_short Upconverting nanoparticle-containing erythrocyte-sized hemoglobin microgels that generate heat, oxygen and reactive oxygen species for suppressing hypoxic tumors
title_sort upconverting nanoparticle-containing erythrocyte-sized hemoglobin microgels that generate heat, oxygen and reactive oxygen species for suppressing hypoxic tumors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9526021/
https://www.ncbi.nlm.nih.gov/pubmed/36203958
http://dx.doi.org/10.1016/j.bioactmat.2022.09.020
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