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A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins
Protein fusions are frequently used for fluorescence imaging of individual molecules, both in vivo and in vitro. The SNAP, CLIP, HALO (aka HaloTag7), and DHFR protein tags can be linked to small molecule dyes that provide brightness and photo-stability superior to fluorescent proteins. To facilitate...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9526040/ https://www.ncbi.nlm.nih.gov/pubmed/35944214 http://dx.doi.org/10.1093/g3journal/jkac201 |
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author | Baek, Inwha Le, Sarah N Jeon, Jongcheol Chun, Yujin Reed, Charlotte Buratowski, Stephen |
author_facet | Baek, Inwha Le, Sarah N Jeon, Jongcheol Chun, Yujin Reed, Charlotte Buratowski, Stephen |
author_sort | Baek, Inwha |
collection | PubMed |
description | Protein fusions are frequently used for fluorescence imaging of individual molecules, both in vivo and in vitro. The SNAP, CLIP, HALO (aka HaloTag7), and DHFR protein tags can be linked to small molecule dyes that provide brightness and photo-stability superior to fluorescent proteins. To facilitate fluorescent dye tagging of proteins in the yeast Saccharomyces cerevisiae, we constructed a modular set of vectors with various combinations of labeling protein tags and selectable markers. These vectors can be used in combination to create strains where multiple proteins labeled with different colored dyes can be simultaneously observed. |
format | Online Article Text |
id | pubmed-9526040 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-95260402022-10-03 A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins Baek, Inwha Le, Sarah N Jeon, Jongcheol Chun, Yujin Reed, Charlotte Buratowski, Stephen G3 (Bethesda) Investigation Protein fusions are frequently used for fluorescence imaging of individual molecules, both in vivo and in vitro. The SNAP, CLIP, HALO (aka HaloTag7), and DHFR protein tags can be linked to small molecule dyes that provide brightness and photo-stability superior to fluorescent proteins. To facilitate fluorescent dye tagging of proteins in the yeast Saccharomyces cerevisiae, we constructed a modular set of vectors with various combinations of labeling protein tags and selectable markers. These vectors can be used in combination to create strains where multiple proteins labeled with different colored dyes can be simultaneously observed. Oxford University Press 2022-08-09 /pmc/articles/PMC9526040/ /pubmed/35944214 http://dx.doi.org/10.1093/g3journal/jkac201 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Genetics Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Investigation Baek, Inwha Le, Sarah N Jeon, Jongcheol Chun, Yujin Reed, Charlotte Buratowski, Stephen A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins |
title | A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins |
title_full | A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins |
title_fullStr | A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins |
title_full_unstemmed | A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins |
title_short | A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins |
title_sort | set of saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins |
topic | Investigation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9526040/ https://www.ncbi.nlm.nih.gov/pubmed/35944214 http://dx.doi.org/10.1093/g3journal/jkac201 |
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