A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins

Protein fusions are frequently used for fluorescence imaging of individual molecules, both in vivo and in vitro. The SNAP, CLIP, HALO (aka HaloTag7), and DHFR protein tags can be linked to small molecule dyes that provide brightness and photo-stability superior to fluorescent proteins. To facilitate...

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Detalles Bibliográficos
Autores principales: Baek, Inwha, Le, Sarah N, Jeon, Jongcheol, Chun, Yujin, Reed, Charlotte, Buratowski, Stephen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Investigation
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9526040/
https://www.ncbi.nlm.nih.gov/pubmed/35944214
http://dx.doi.org/10.1093/g3journal/jkac201
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author Baek, Inwha
Le, Sarah N
Jeon, Jongcheol
Chun, Yujin
Reed, Charlotte
Buratowski, Stephen
author_facet Baek, Inwha
Le, Sarah N
Jeon, Jongcheol
Chun, Yujin
Reed, Charlotte
Buratowski, Stephen
author_sort Baek, Inwha
collection PubMed
description Protein fusions are frequently used for fluorescence imaging of individual molecules, both in vivo and in vitro. The SNAP, CLIP, HALO (aka HaloTag7), and DHFR protein tags can be linked to small molecule dyes that provide brightness and photo-stability superior to fluorescent proteins. To facilitate fluorescent dye tagging of proteins in the yeast Saccharomyces cerevisiae, we constructed a modular set of vectors with various combinations of labeling protein tags and selectable markers. These vectors can be used in combination to create strains where multiple proteins labeled with different colored dyes can be simultaneously observed.
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spelling pubmed-95260402022-10-03 A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins Baek, Inwha Le, Sarah N Jeon, Jongcheol Chun, Yujin Reed, Charlotte Buratowski, Stephen G3 (Bethesda) Investigation Protein fusions are frequently used for fluorescence imaging of individual molecules, both in vivo and in vitro. The SNAP, CLIP, HALO (aka HaloTag7), and DHFR protein tags can be linked to small molecule dyes that provide brightness and photo-stability superior to fluorescent proteins. To facilitate fluorescent dye tagging of proteins in the yeast Saccharomyces cerevisiae, we constructed a modular set of vectors with various combinations of labeling protein tags and selectable markers. These vectors can be used in combination to create strains where multiple proteins labeled with different colored dyes can be simultaneously observed. Oxford University Press 2022-08-09 /pmc/articles/PMC9526040/ /pubmed/35944214 http://dx.doi.org/10.1093/g3journal/jkac201 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Genetics Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigation
Baek, Inwha
Le, Sarah N
Jeon, Jongcheol
Chun, Yujin
Reed, Charlotte
Buratowski, Stephen
A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins
title A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins
title_full A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins
title_fullStr A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins
title_full_unstemmed A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins
title_short A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins
title_sort set of saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins
topic Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9526040/
https://www.ncbi.nlm.nih.gov/pubmed/35944214
http://dx.doi.org/10.1093/g3journal/jkac201
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