Preparation of a murine oral palate wound healing model

The oral cavity is a highly regenerative epithelial tissue that results in minimal scarring after injury. This protocol describes the preparation of a mouse palate wound model. The protocol includes steps to place an excisional wound on the mouse palate, followed by harvesting of wound tissue and bo...

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Detalles Bibliográficos
Autores principales: Leonardo, Trevor R., Chen, Lin, DiPietro, Luisa A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9526232/
https://www.ncbi.nlm.nih.gov/pubmed/36170111
http://dx.doi.org/10.1016/j.xpro.2022.101727
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author Leonardo, Trevor R.
Chen, Lin
DiPietro, Luisa A.
author_facet Leonardo, Trevor R.
Chen, Lin
DiPietro, Luisa A.
author_sort Leonardo, Trevor R.
collection PubMed
description The oral cavity is a highly regenerative epithelial tissue that results in minimal scarring after injury. This protocol describes the preparation of a mouse palate wound model. The protocol includes steps to place an excisional wound on the mouse palate, followed by harvesting of wound tissue and bone decalcification. We detail how to overcome the technical challenge of limited anatomical space, avoid damaging the nasal cavity, manage bleeding, and collect tissue for downstream genomic or immunohistochemical analysis.
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spelling pubmed-95262322022-10-02 Preparation of a murine oral palate wound healing model Leonardo, Trevor R. Chen, Lin DiPietro, Luisa A. STAR Protoc Protocol The oral cavity is a highly regenerative epithelial tissue that results in minimal scarring after injury. This protocol describes the preparation of a mouse palate wound model. The protocol includes steps to place an excisional wound on the mouse palate, followed by harvesting of wound tissue and bone decalcification. We detail how to overcome the technical challenge of limited anatomical space, avoid damaging the nasal cavity, manage bleeding, and collect tissue for downstream genomic or immunohistochemical analysis. Elsevier 2022-09-27 /pmc/articles/PMC9526232/ /pubmed/36170111 http://dx.doi.org/10.1016/j.xpro.2022.101727 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Leonardo, Trevor R.
Chen, Lin
DiPietro, Luisa A.
Preparation of a murine oral palate wound healing model
title Preparation of a murine oral palate wound healing model
title_full Preparation of a murine oral palate wound healing model
title_fullStr Preparation of a murine oral palate wound healing model
title_full_unstemmed Preparation of a murine oral palate wound healing model
title_short Preparation of a murine oral palate wound healing model
title_sort preparation of a murine oral palate wound healing model
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9526232/
https://www.ncbi.nlm.nih.gov/pubmed/36170111
http://dx.doi.org/10.1016/j.xpro.2022.101727
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AT chenlin preparationofamurineoralpalatewoundhealingmodel
AT dipietroluisaa preparationofamurineoralpalatewoundhealingmodel

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