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Cross talk of vasopressin conditioned cell therapy in ischemic heart disease: Role of oxidative stress markers

OBJECTIVE(S): Background: Impaired coronary blood flow causes cardiac ischemia. Cellular therapy is a new approach to the treatment of myocardial ischemia. This study aimed to investigate the effect of adipose tissue-derived mesenchymal stem cells (AD-MSCs) conditioned with vasopressin on oxidative...

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Autores principales: Bagheri, Mona, Nasiri Boroujeni, Shakiba, Ahmadvand, Hassan, Nazari, Afshin, Chehelcheraghi, Farzaneh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9526888/
https://www.ncbi.nlm.nih.gov/pubmed/36246071
http://dx.doi.org/10.22038/IJBMS.2022.62540.13837
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author Bagheri, Mona
Nasiri Boroujeni, Shakiba
Ahmadvand, Hassan
Nazari, Afshin
Chehelcheraghi, Farzaneh
author_facet Bagheri, Mona
Nasiri Boroujeni, Shakiba
Ahmadvand, Hassan
Nazari, Afshin
Chehelcheraghi, Farzaneh
author_sort Bagheri, Mona
collection PubMed
description OBJECTIVE(S): Background: Impaired coronary blood flow causes cardiac ischemia. Cellular therapy is a new approach to the treatment of myocardial ischemia. This study aimed to investigate the effect of adipose tissue-derived mesenchymal stem cells (AD-MSCs) conditioned with vasopressin on oxidative stress, perivascular collagen, and angiogenesis caused by myocardial infarction (MI) in rats. MATERIALS AND METHODS: We divided 40 male albino Wistar rats into 4 groups; Control group; No intervention; in experimental groups, after it generated induced MI on models, it divided into three groups: Vehicle group (150 μl of cell-free culture medium received); ASC-MI group (6× 10(6) AD-MSC received) and AVP-ASC-MI group (received 6 × 10(6) AD-MSC conditioned with 10 nM vasopressin). Then, histologic parameters and anti-oxidant enzymes were evaluated 7 days post-MI cell injection. RESULTS: Arterial muscle diameter improved and collagen deposition around the coronary arteries decreased in cell-received groups compared with the vehicle group. Malondialdehyde (MDA), catalase (CAT), (GSH) Glutathione, and Total Anti-oxidant Capacity (TAC) parameters were not significantly different between the cells received groups compared with the vehicle group. But the Catalase (CAT) parameter in the ASC-MI group had a significant increase from the control group. CONCLUSION: We prepared direct evidence that intramyocardial injection of AD-MSCs reveals the positive cardiac remodeling post-MI in rats, and these useful effects can be more enhanced by administrating injection of conditioned ADSCs with vasopressin.
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spelling pubmed-95268882022-10-13 Cross talk of vasopressin conditioned cell therapy in ischemic heart disease: Role of oxidative stress markers Bagheri, Mona Nasiri Boroujeni, Shakiba Ahmadvand, Hassan Nazari, Afshin Chehelcheraghi, Farzaneh Iran J Basic Med Sci Original Article OBJECTIVE(S): Background: Impaired coronary blood flow causes cardiac ischemia. Cellular therapy is a new approach to the treatment of myocardial ischemia. This study aimed to investigate the effect of adipose tissue-derived mesenchymal stem cells (AD-MSCs) conditioned with vasopressin on oxidative stress, perivascular collagen, and angiogenesis caused by myocardial infarction (MI) in rats. MATERIALS AND METHODS: We divided 40 male albino Wistar rats into 4 groups; Control group; No intervention; in experimental groups, after it generated induced MI on models, it divided into three groups: Vehicle group (150 μl of cell-free culture medium received); ASC-MI group (6× 10(6) AD-MSC received) and AVP-ASC-MI group (received 6 × 10(6) AD-MSC conditioned with 10 nM vasopressin). Then, histologic parameters and anti-oxidant enzymes were evaluated 7 days post-MI cell injection. RESULTS: Arterial muscle diameter improved and collagen deposition around the coronary arteries decreased in cell-received groups compared with the vehicle group. Malondialdehyde (MDA), catalase (CAT), (GSH) Glutathione, and Total Anti-oxidant Capacity (TAC) parameters were not significantly different between the cells received groups compared with the vehicle group. But the Catalase (CAT) parameter in the ASC-MI group had a significant increase from the control group. CONCLUSION: We prepared direct evidence that intramyocardial injection of AD-MSCs reveals the positive cardiac remodeling post-MI in rats, and these useful effects can be more enhanced by administrating injection of conditioned ADSCs with vasopressin. Mashhad University of Medical Sciences 2022-09 /pmc/articles/PMC9526888/ /pubmed/36246071 http://dx.doi.org/10.22038/IJBMS.2022.62540.13837 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Bagheri, Mona
Nasiri Boroujeni, Shakiba
Ahmadvand, Hassan
Nazari, Afshin
Chehelcheraghi, Farzaneh
Cross talk of vasopressin conditioned cell therapy in ischemic heart disease: Role of oxidative stress markers
title Cross talk of vasopressin conditioned cell therapy in ischemic heart disease: Role of oxidative stress markers
title_full Cross talk of vasopressin conditioned cell therapy in ischemic heart disease: Role of oxidative stress markers
title_fullStr Cross talk of vasopressin conditioned cell therapy in ischemic heart disease: Role of oxidative stress markers
title_full_unstemmed Cross talk of vasopressin conditioned cell therapy in ischemic heart disease: Role of oxidative stress markers
title_short Cross talk of vasopressin conditioned cell therapy in ischemic heart disease: Role of oxidative stress markers
title_sort cross talk of vasopressin conditioned cell therapy in ischemic heart disease: role of oxidative stress markers
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9526888/
https://www.ncbi.nlm.nih.gov/pubmed/36246071
http://dx.doi.org/10.22038/IJBMS.2022.62540.13837
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