Cargando…
(99m)Tc-labeled iRGD for single-positron emission computed tomography imaging of triple-negative breast cancer
Triple-negative breast cancer (TNBC) is the most aggressive breast cancer subtype, with a high mortality rate. One of the main reasons for this poor prognosis is the failure of a specific diagnosis. As a tumor-homing and penetrating peptide, iRGD has not only the properties of binding to neuropilin-...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9527319/ https://www.ncbi.nlm.nih.gov/pubmed/36199363 http://dx.doi.org/10.3389/fbioe.2022.1001899 |
_version_ | 1784801058793455616 |
---|---|
author | Yu, Buhui Su, Hongxing Zhao, Lingzhou Yang, Jiqin Zhu, Meilin Zhao, Jinhua |
author_facet | Yu, Buhui Su, Hongxing Zhao, Lingzhou Yang, Jiqin Zhu, Meilin Zhao, Jinhua |
author_sort | Yu, Buhui |
collection | PubMed |
description | Triple-negative breast cancer (TNBC) is the most aggressive breast cancer subtype, with a high mortality rate. One of the main reasons for this poor prognosis is the failure of a specific diagnosis. As a tumor-homing and penetrating peptide, iRGD has not only the properties of binding to neuropilin-1 and integrin αvβ3 but also internalizing into TNBC cells. In this study, we designed and prepared (99m)Tc-labeled iRGD ((99m)Tc-HYNIC-iRGD) as a single-positron emission computed tomography (SPECT) imaging probe and investigated its feasibility for the targeted diagnosis of TNBC. The results showed that the iRGD peptide had acceptable biocompatibility within the studied concentration range and could specifically bind to TNBC cells in vitro. The (99m)Tc-HYNIC-iRGD was readily prepared with high radiochemical purity and stability. SPECT imaging of (99m)Tc-HYNIC-iRGD in a TNBC tumor-bearing mouse model showed obvious tumor accumulation with rapid blood clearance and favorable biodistribution. Our findings indicate that this active-targeted strategy has great potential to be developed as a novel tool for TNBC imaging. |
format | Online Article Text |
id | pubmed-9527319 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95273192022-10-04 (99m)Tc-labeled iRGD for single-positron emission computed tomography imaging of triple-negative breast cancer Yu, Buhui Su, Hongxing Zhao, Lingzhou Yang, Jiqin Zhu, Meilin Zhao, Jinhua Front Bioeng Biotechnol Bioengineering and Biotechnology Triple-negative breast cancer (TNBC) is the most aggressive breast cancer subtype, with a high mortality rate. One of the main reasons for this poor prognosis is the failure of a specific diagnosis. As a tumor-homing and penetrating peptide, iRGD has not only the properties of binding to neuropilin-1 and integrin αvβ3 but also internalizing into TNBC cells. In this study, we designed and prepared (99m)Tc-labeled iRGD ((99m)Tc-HYNIC-iRGD) as a single-positron emission computed tomography (SPECT) imaging probe and investigated its feasibility for the targeted diagnosis of TNBC. The results showed that the iRGD peptide had acceptable biocompatibility within the studied concentration range and could specifically bind to TNBC cells in vitro. The (99m)Tc-HYNIC-iRGD was readily prepared with high radiochemical purity and stability. SPECT imaging of (99m)Tc-HYNIC-iRGD in a TNBC tumor-bearing mouse model showed obvious tumor accumulation with rapid blood clearance and favorable biodistribution. Our findings indicate that this active-targeted strategy has great potential to be developed as a novel tool for TNBC imaging. Frontiers Media S.A. 2022-09-19 /pmc/articles/PMC9527319/ /pubmed/36199363 http://dx.doi.org/10.3389/fbioe.2022.1001899 Text en Copyright © 2022 Yu, Su, Zhao, Yang, Zhu and Zhao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Yu, Buhui Su, Hongxing Zhao, Lingzhou Yang, Jiqin Zhu, Meilin Zhao, Jinhua (99m)Tc-labeled iRGD for single-positron emission computed tomography imaging of triple-negative breast cancer |
title |
(99m)Tc-labeled iRGD for single-positron emission computed tomography imaging of triple-negative breast cancer |
title_full |
(99m)Tc-labeled iRGD for single-positron emission computed tomography imaging of triple-negative breast cancer |
title_fullStr |
(99m)Tc-labeled iRGD for single-positron emission computed tomography imaging of triple-negative breast cancer |
title_full_unstemmed |
(99m)Tc-labeled iRGD for single-positron emission computed tomography imaging of triple-negative breast cancer |
title_short |
(99m)Tc-labeled iRGD for single-positron emission computed tomography imaging of triple-negative breast cancer |
title_sort | (99m)tc-labeled irgd for single-positron emission computed tomography imaging of triple-negative breast cancer |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9527319/ https://www.ncbi.nlm.nih.gov/pubmed/36199363 http://dx.doi.org/10.3389/fbioe.2022.1001899 |
work_keys_str_mv | AT yubuhui 99mtclabeledirgdforsinglepositronemissioncomputedtomographyimagingoftriplenegativebreastcancer AT suhongxing 99mtclabeledirgdforsinglepositronemissioncomputedtomographyimagingoftriplenegativebreastcancer AT zhaolingzhou 99mtclabeledirgdforsinglepositronemissioncomputedtomographyimagingoftriplenegativebreastcancer AT yangjiqin 99mtclabeledirgdforsinglepositronemissioncomputedtomographyimagingoftriplenegativebreastcancer AT zhumeilin 99mtclabeledirgdforsinglepositronemissioncomputedtomographyimagingoftriplenegativebreastcancer AT zhaojinhua 99mtclabeledirgdforsinglepositronemissioncomputedtomographyimagingoftriplenegativebreastcancer |