Cargando…

Antitumor Potential of Sericite Treatment Mediated by Cell Cycle Arrest in Triple-Negative MDA-MB231 Breast Cancer Cells

Breast cancer is the most common cancer and the leading cause of cancer-related mortality among females worldwide. Triple-negative breast cancer (TNBC) accounts for about 10–15% of all breast cancers and is usually more aggressive and has a poorer prognosis. Sericite has been known to have antitumor...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Seonhee, Nagar, Harsha, Lee, Ikjun, Choi, Su-Jeong, Piao, Shuyu, Jeon, Byeong Hwa, Kang, Shin Kwang, Song, Hee-Jung, Kim, Cuk-Seong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9527418/
https://www.ncbi.nlm.nih.gov/pubmed/36199546
http://dx.doi.org/10.1155/2022/2885293
_version_ 1784801083113078784
author Kim, Seonhee
Nagar, Harsha
Lee, Ikjun
Choi, Su-Jeong
Piao, Shuyu
Jeon, Byeong Hwa
Kang, Shin Kwang
Song, Hee-Jung
Kim, Cuk-Seong
author_facet Kim, Seonhee
Nagar, Harsha
Lee, Ikjun
Choi, Su-Jeong
Piao, Shuyu
Jeon, Byeong Hwa
Kang, Shin Kwang
Song, Hee-Jung
Kim, Cuk-Seong
author_sort Kim, Seonhee
collection PubMed
description Breast cancer is the most common cancer and the leading cause of cancer-related mortality among females worldwide. Triple-negative breast cancer (TNBC) accounts for about 10–15% of all breast cancers and is usually more aggressive and has a poorer prognosis. Sericite has been known to have antitumor and immune-stimulatory effects. Although the chemopreventive potential of sericite has been demonstrated in other cancers, its molecular pathways in TNBC still require investigation. Thus, in the present study, the antitumor mechanism of sericite against MDA-MB231 breast cancer cells was examined in vitro and in an in vivo xenograft mouse model. Sericite treatment reduced cell proliferation and cell proliferation marker proliferating cell nuclear antigen (PCNA) in MDA-MB231 cells. It also decreased the total cell number and arrested cells in the G0/G1 phase of the cell cycle with an increase in the phosphorylation of P53 and upregulation of cell cycle regulatory proteins P21 and P16. In addition, sericite treatment also induced apoptosis signaling, which was evident by the upregulation of apoptotic protein markers cleaved caspases 3 and 9. A reduction in reactive oxygen species (ROS), NADPH oxidase 4 (NOX4), p22phox, and heat shock proteins (HSPs) was also observed. Similar results were obtained in vivo with significantly reduced tumor volume in sericite-administered mice. Collectively, these findings suggest that sericite has antitumor potential based on its property to induce cell cycle arrest and apoptotic cell death and therefore could serve as a potential therapeutic agent and crucial candidate in anticancer drug development for TNBC.
format Online
Article
Text
id pubmed-9527418
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-95274182022-10-04 Antitumor Potential of Sericite Treatment Mediated by Cell Cycle Arrest in Triple-Negative MDA-MB231 Breast Cancer Cells Kim, Seonhee Nagar, Harsha Lee, Ikjun Choi, Su-Jeong Piao, Shuyu Jeon, Byeong Hwa Kang, Shin Kwang Song, Hee-Jung Kim, Cuk-Seong Evid Based Complement Alternat Med Research Article Breast cancer is the most common cancer and the leading cause of cancer-related mortality among females worldwide. Triple-negative breast cancer (TNBC) accounts for about 10–15% of all breast cancers and is usually more aggressive and has a poorer prognosis. Sericite has been known to have antitumor and immune-stimulatory effects. Although the chemopreventive potential of sericite has been demonstrated in other cancers, its molecular pathways in TNBC still require investigation. Thus, in the present study, the antitumor mechanism of sericite against MDA-MB231 breast cancer cells was examined in vitro and in an in vivo xenograft mouse model. Sericite treatment reduced cell proliferation and cell proliferation marker proliferating cell nuclear antigen (PCNA) in MDA-MB231 cells. It also decreased the total cell number and arrested cells in the G0/G1 phase of the cell cycle with an increase in the phosphorylation of P53 and upregulation of cell cycle regulatory proteins P21 and P16. In addition, sericite treatment also induced apoptosis signaling, which was evident by the upregulation of apoptotic protein markers cleaved caspases 3 and 9. A reduction in reactive oxygen species (ROS), NADPH oxidase 4 (NOX4), p22phox, and heat shock proteins (HSPs) was also observed. Similar results were obtained in vivo with significantly reduced tumor volume in sericite-administered mice. Collectively, these findings suggest that sericite has antitumor potential based on its property to induce cell cycle arrest and apoptotic cell death and therefore could serve as a potential therapeutic agent and crucial candidate in anticancer drug development for TNBC. Hindawi 2022-09-25 /pmc/articles/PMC9527418/ /pubmed/36199546 http://dx.doi.org/10.1155/2022/2885293 Text en Copyright © 2022 Seonhee Kim et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kim, Seonhee
Nagar, Harsha
Lee, Ikjun
Choi, Su-Jeong
Piao, Shuyu
Jeon, Byeong Hwa
Kang, Shin Kwang
Song, Hee-Jung
Kim, Cuk-Seong
Antitumor Potential of Sericite Treatment Mediated by Cell Cycle Arrest in Triple-Negative MDA-MB231 Breast Cancer Cells
title Antitumor Potential of Sericite Treatment Mediated by Cell Cycle Arrest in Triple-Negative MDA-MB231 Breast Cancer Cells
title_full Antitumor Potential of Sericite Treatment Mediated by Cell Cycle Arrest in Triple-Negative MDA-MB231 Breast Cancer Cells
title_fullStr Antitumor Potential of Sericite Treatment Mediated by Cell Cycle Arrest in Triple-Negative MDA-MB231 Breast Cancer Cells
title_full_unstemmed Antitumor Potential of Sericite Treatment Mediated by Cell Cycle Arrest in Triple-Negative MDA-MB231 Breast Cancer Cells
title_short Antitumor Potential of Sericite Treatment Mediated by Cell Cycle Arrest in Triple-Negative MDA-MB231 Breast Cancer Cells
title_sort antitumor potential of sericite treatment mediated by cell cycle arrest in triple-negative mda-mb231 breast cancer cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9527418/
https://www.ncbi.nlm.nih.gov/pubmed/36199546
http://dx.doi.org/10.1155/2022/2885293
work_keys_str_mv AT kimseonhee antitumorpotentialofsericitetreatmentmediatedbycellcyclearrestintriplenegativemdamb231breastcancercells
AT nagarharsha antitumorpotentialofsericitetreatmentmediatedbycellcyclearrestintriplenegativemdamb231breastcancercells
AT leeikjun antitumorpotentialofsericitetreatmentmediatedbycellcyclearrestintriplenegativemdamb231breastcancercells
AT choisujeong antitumorpotentialofsericitetreatmentmediatedbycellcyclearrestintriplenegativemdamb231breastcancercells
AT piaoshuyu antitumorpotentialofsericitetreatmentmediatedbycellcyclearrestintriplenegativemdamb231breastcancercells
AT jeonbyeonghwa antitumorpotentialofsericitetreatmentmediatedbycellcyclearrestintriplenegativemdamb231breastcancercells
AT kangshinkwang antitumorpotentialofsericitetreatmentmediatedbycellcyclearrestintriplenegativemdamb231breastcancercells
AT songheejung antitumorpotentialofsericitetreatmentmediatedbycellcyclearrestintriplenegativemdamb231breastcancercells
AT kimcukseong antitumorpotentialofsericitetreatmentmediatedbycellcyclearrestintriplenegativemdamb231breastcancercells